Single-cell genomics technologies have accelerated our understanding of cell-state heterogeneity in diverse contexts. Although single-cell RNA sequencing (scRNA-seq) identifies many rare populations...

The Lareau Laboratory for Computational and Translation Immunology is housed in the csBio Program at Memorial Sloan Kettering Cancer Center, New York, NY.

Cell therapies have yielded durable clinical benefits for patients with cancer, but the risks associated with the development of therapies from manipulated human cells are still being understood. For...

Nature Biotechnology - Chromatin accessibility, gene expression and protein levels are measured in the same single cell.

Nature Genetics - Single-cell analyses of immune cells from patients with pathogenic, single large-scale mitochondrial DNA (mtDNA) deletions including Pearson syndrome describe heteroplasmy...

Many mitochondrial diseases are caused by mutations in mitochondrial DNA (mtDNA). Patients’ cells contain a mixture of mutant and nonmutant mtDNA (a phenomenon called heteroplasmy). The proportion ...

A widespread assumption for single-cell analyses specifies that one cell’s nucleic acids are predominantly captured by one oligonucleotide barcode. However, we show that ~13-21% of cell barcodes from...

Somatic variation contributes to biological heterogeneity by modulating cellular proclivity to differentiate, expand, adapt, or die. While large-scale sequencing efforts have revealed the foundatio...

Inventing the future from the atom up.

Nature - CRISPR DNA base editors induce transcriptome-wide off-target RNA editing, which can be reduced by using engineered variants that retain on-target DNA editing activities.

The Computational & Systems Biology Program at MSK seeks innovative investigators for tenured or tenure-track positions

The specificity of antibodies makes cancer immunotherapies, including chimeric antigen receptor T cells and antibody-drug conjugates, possible. In parallel, single-cell genomics technologies present...

Nature Communications - It is assumed that single-cell analyses capture one barcode per cell. Here, the authors show that up to 21% of cell barcodes on the 10X Chromium scATAC-seq assay may be...

Stanford University is seeking a Life Science Research Professional 1 to perform basic functions and activities involved in defined research...

The Lareau Laboratory for Computational and Translation Immunology is housed in the csBio Program at Memorial Sloan Kettering Cancer Center, New York, NY.

Confusion developed in a 49-year-old woman with relapsed diffuse large B-cell lymphoma within days after she received CAR-T therapy. She had difficulty putting words together and was disoriented bu...

Our previous publication suggested CRISPR-Cas9 editing at the zygotic stage might unexpectedly introduce a multitude of subtle but unintended mutations, an interpretation that not surprisingly raised...

Nature Biotechnology - Combining droplet-based ATAC-seq and mitochondrial DNA sequencing reveals clonal variation in human cells and tissues.

While recent technical advancements have facilitated the mapping of epigenomes at single-cell resolution, the throughput and quality of these methods have limited the widespread adoption of these...

Nature Methods - hichipper: a preprocessing pipeline for calling DNA loops from HiChIP data

Background Genome-wide association studies (GWAS) are typically visualized using a two-dimensional Manhattan plot, displaying chromosomal location of SNPs along the x-axis and the negative log-10 of...