Amélie Fréal
@FreAmeLie
Followers
810
Following
481
Media
53
Statuses
585
Cell biologist, fascinated by neurons and their cytoskeleton(s), AIS fan, mother of 2 and group leader @FGA_lab
Utrecht, Pays-Bas
Joined July 2012
Don't wanna be here?
Send us removal request.
Explore trending content on Musk Viewer
Mbappe
• 468326 Tweets
Lukaku
• 172311 Tweets
Kante
• 170357 Tweets
Dudu
• 107750 Tweets
Belgium
• 97361 Tweets
Cruzeiro
• 92970 Tweets
Thuram
• 81818 Tweets
Katy Perry
• 72129 Tweets
#AUTFRA
• 70835 Tweets
Jack Black
• 66168 Tweets
Leila
• 63611 Tweets
Bélgica
• 46776 Tweets
Ceci Flores
• 43402 Tweets
#FRAAUT
• 42234 Tweets
Joey
• 35841 Tweets
ニンダイ
• 34703 Tweets
Saliba
• 32595 Tweets
CONGRATULATIONS JUNGKOOK
• 32049 Tweets
大雨警報
• 28320 Tweets
Dembele
• 27096 Tweets
Deschamps
• 25567 Tweets
Samantha
• 24196 Tweets
الزمالك
• 22158 Tweets
Griezmann
• 21435 Tweets
Camavinga
• 20781 Tweets
Rabiot
• 18680 Tweets
كانتي
• 16075 Tweets
土砂降り
• 14988 Tweets
Giroud
• 12308 Tweets
オーストリア
• 10818 Tweets
Nancy Mackenzie
• 10383 Tweets
Over the moon that
@ERC_Research
will fund our research on Axon Initial Segment plasticity
#ERCStG
! There will be positions available, get in touch if you are a microscopy enthusiast, cell bio and neuro fan!
@FGA_lab
@amsterdamumc
@VUamsterdam
26
17
168
And it is out! 🥳🥳We spent a few years addressing the mechanisms of rapid AIS plasticity. We wanted to understand how sodium channels (Nav) are re-organized during AIS remodeling.
9
24
159
I am recruiting! If you are looking for a PhD position and interested in molecular/cellular neuroscience, consider applying 👇RT appreciated! And happy holiday!
@amsterdamumc
@AmsNeuroscience
@GeneticAMS
@FGA_lab
@ERC_Research
3
92
150
I feel fortunate to have a job I love and to work in a safe environment. And I am very happy to share that I will start my lab
@FGA_lab
@VUamsterdam
soon, to dig into the molecular biology of the Axon Initial Segment plasticity.
21
8
116
I am hiring soon (PhD and post-doc) and will be at
#SfN23
in November! Ping me to grab a drink and talk about the AIS, our lab, living in The Netherlands, being a parent in academia or anything related to food/wine/cheese!
4
36
100
Wow! Watching it and thinking that even during weekends I really love my job 😍! Beautiful neurons and even EB-comets! Thanks
@cellworlds_off
- Bassins des lumières, Bordeaux
3
2
77
It's out!!🎉Happy to share my work revealing the interplay between the membrane-associated scaffold 480AnkyrinG and the microtubule bundler TRIM46 in the process of AIS formation.
@UniUtrecht
@NIN_knaw
@NeuroCellPress
7
11
60
Happy to share our latest work on AIS plasticity! 🥳
In this study, we addressed the molecular mechanisms of rapid AIS plasticity, focusing on how Nav channels are reorganized.
@UniUtrecht
@NIN_knaw
@FGA_lab
6
13
60
It's a double
#onthecover
!! Lab is literally ON the cover!
@DieudonneeW
@feline_lindhout
@MartinHarterink
The November issue is online:
—>
The cover shows a lab-specific rendition of Rembrandt highlighting work by Anna Akhmanov
@UniUtrecht
and co-workers describing that Gas2L1 stimulates
#axon
branching and reduces axon outgrowth.
—>
1
33
89
2
5
52
Come for the science, stay for the g̶o̶o̶d̶ ̶f̶o̶o̶d̶, n̶i̶c̶e̶ ̶w̶e̶a̶t̶h̶e̶r̶, fun science, great people & bicycles! (Amsterdam is great and there is actually some decent cheese!) Here is the vacancy, join us!
@FGA_lab
@VUamsterdam
0
19
37
Kids went on vacation this morning
Me: "let's take some time for myself"
...
Me again: "let's book all the microscopes of the imaging facility next week"
2
0
34
Last month Liu He defended her PhD and I had the privilege to be part of the committee. Liu did amazing,
@MartinHarterink
can be proud! And thanks to
@UniUtrecht
new policy, I got the chance to wear a toge and I have to say that it made me quite happy 😊
#WomenInSTEM
0
0
33
We are looking for a group leader (tenure track) in cellular neuroscience! If you want to develop your research line in a great environment (obviously with great colleagues!) in Amsterdam, apply! 👇
2
19
30
Interesting read! ALS hiPSC motor neurons have longer AIS, with altered AIS plasticity, preventing them to homeostatically regulate their excitability.
Aberrant axon initial segment plasticity and intrinsic excitability of ALS hiPSC motor neurons
0
20
76
0
4
25
Come work with us! PhD student position in neuron-microglia physiology
0
25
22
I was very happy to present our research in front of such a great crowd!!
What a cool day organized by
@AmsNeuroscience
@FreAmeLie
all geared up to present at the
@AmsNeuroscience
annual meeting! We are looking forward to a day of excellent science!
@FGA_lab
@McnLab
0
1
7
0
1
19
The Dutch Neuroscience Meeting is over! Great talks and posters, lots of science and fun discussions. I feel lucky to be part of this happy community 😊
#DNM22
#newPI
And
@NoraJamann
made the cover!
0
0
18
8
0
17
I will be hiring soon, so get in touch if you are interested in live-cell and super-resolution imaging of the coolest (yes!) neuronal compartment!
1
5
15
EB comets at Utrecht station!
I-Light-U EXPO, from Vendel en de Wolf
2
0
20
Back to school! I had forgotten how it feels to sit on this side of the classroom 🤓
2
0
16
I will do my best to offer other people a safe place to do fun science and as much support as I got from everyone
@NIN_knaw
and
@UniUtrecht
to get there!
1
0
14
Come work with us at the
@NIN_knaw
in Amsterdam! The Kole lab is recruiting a PhD Student to investigate the mechanisms of myelin pathology in preclinical models of MS.
More details here 👇
Great project & very cool group (with nice colleagues 😉)
0
8
13
Yesterday was the first time I was part of a PhD committee, and Jessie did so great! But it was also exactly 10 years after I defended my own PhD! Time flies!
#PhDversary
Today Jessie Brunner defended her thesis, ‘Human synapses: IPSC derived neurons as a model to study human synapse formation and function’. We are so proud🎉🎉
@VUamsterdam
@amsterdamumc
@GeneticAMS
0
0
16
0
0
13
A European perspective on structural barriers to women’s career progression in neuroscience | Nature Neuroscience
0
5
12
Fan of live cell imaging? Come work with us!
We are hiring. Looking for an job with challenging live cell microscopy techniques in a dynamic team? Check the job description;
0
4
4
0
5
11
Last minute birthday champagne! Thank you
@kahn_olga
@MartinHarterink
@feline_lindhout
@hdmacgillavry
1
0
11
It has been an amazing journey, I've learned so much, used almost all the microscopes of our facility and got to work with amazing scientists! Thanks to
@Dipti_Rai_
@roderick_tas
@XingxiuPan
@katpyxa
@DieudonneeW
@MartinHarterink
@KapiteinLukas
, Anna Akhmanova & Casper Hoogenraad
3
0
9
My life in 7(-in-1) pictures. Day 1(to 7). No words, no people and challenge a new person every day. I was challenged by
@AndrieuxLab
and I challenge
@NoraJamann
@naomihanemaayer
@feline_lindhout
@NickyScheefhals
@LisaCatsburg
@XingxiuPan
@DieudonneeW
1
0
9
I am extremely proud of this collaborative work, combining electrophysiological recordings with live-cell and super-resolution imaging of endogenously tagged proteins to advance our knowledge of AIS plasticity. Big shout out to the brilliant
@NoraJamann
and Maarten Kole!
1
1
8
@psychescope
@christlet
This neurite seems to be slighty enriched in less mobile EB, maybe it's the AIS?! It looks beatiful!
1
0
7
Great tool and easy to use, so many cool questions that can be addressed now!! Well done
@hdmacgillavry
@NickyScheefhals
@Jelmerwillems
We are very excited to present ORANGE!
Open Resource for the Application of Neuronal Genome Editing: a CRISPR/Cas9-based toolbox to label endogenous proteins with fluorescent tags. Our firs preprint:
@Jelmerwillems
@NickyScheefhals
20
134
311
0
0
7
@christlet
Now that the lab is closed,
@KapiteinLukas
@katpyxa
@MartinHarterink
,
@feline_lindhout
,
@hdmacgillavry
, me and others should start training! We might be ready next time
@christlet
visits us!
2
0
7
Nice work by
@Thom_Wolf
“State-of-the-art neural coreference resolution for chatbots” by Thomas Wolf
0
4
7
Great work on AIS plasticity in vivo by
@NoraJamann
@AxonalLab
We begin our lab's Twitter life with this latest work on in vivo AIS plasticity. With
#NoraJamann
0
14
30
0
0
6
Proud to be part of this nice story!
Motor axon navigation relies on Fidgetin-like 1–driven microtubule plus end dynamics
1
1
6
To all past, present and future ERC StG writers: any tips on how to get started??? 😅
#sciencetwitter
#WomenInSTEM
@Momademia
1
4
5
Calcium entry at the AIS via Nav! Great work by
@naomihanemaayer
and
@markocms
in Maarten Kole lab, congratulations!
0
1
5
congrats
@NickyScheefhals
and
@hdmacgillavry
!!
It's out there! Our review on glutamate receptor positioning at excitatory synapses:
with
@NickyScheefhals
3
33
60
1
1
5
@christlet
@Biosdfp
Excellent work!! 🥳
Stunning images and the AIS 'clearings' are spectacular!
Also glad you confirmed what we also observed with
@NoraJamann
that NMDA activates endocytosis at the AIS 😉 your images of those 'woken-up pits' is incredibly convincing!
2
2
5
@christlet
@KapiteinLukas
@hdmacgillavry
@JeroenPasterk
Great to have you here! Can't wait to hear about your new projects!
0
0
5
0
0
3
I am immensely grateful for the hard work done by
@NoraJamann
, Twitter-X-less (still??) Maarten Kole and collaborators. Big thank you to our support and funding
@UUBeta
@UniUtrecht
@NIN_knaw
@VUamsterdam
@amsterdamumc
@NWONieuws
@FGA_lab
0
0
4
Very proud of this collaborative work, where combination of expertise brought us one step further (and made it more fun)! I find particularly exciting that AIS and synaptic plasticity co-occur and share common molecular mechanisms. Clearly, this will be followed up!
1
0
4
Fantastic PhD opportunity, great science and mentor!
Fully funded
#PhD
opportunity! Join us and the awesome
@adilgkhan
to study plasticity in neuronal regeneration here in sunny London as part of the superb King's MRC DTP scheme. Please RT!
0
30
38
0
2
4
We tried (veryyyyyy hard) to visualize internalization of Nav1.2 channels during AIS plasticity. What we saw is the appearance of Nav1.2 vesicles colocalizing with dynamin2, and an increase in endocytic structures at the distal AIS during plasticity in the presence of Dynasore.
1
0
4
@christlet
@Biosdfp
@NoraJamann
Congratulations to everyone involved for this work! This is a very important contribution to the field and was definitely a missing piece of the puzzle!
Exciting times ahead to crack the cell biology behind activity-regulated endocytosis at the AIS 😀
1
1
4
@CellReports
Congratulations
@RobinBuijs1
!! Cool story from the lab on the role of WDR47 in protecting microtubule minus ends from katanin severing in neurons!
0
0
4
@mariescopy
@HenriquesLab
Moved to the NL with my 1.5yo, first 3 months have been frustrating for him, but now he's fluent in Dutch! Going to a bilingual or international school might help, because teachers are aware of language issues/integration and kids are used to switch between several languages!
0
0
4
In short,
1. We reveal a new function for 480AnkyrinG. Once targeted to the membrane, 480AnkyrinG efficiently recruits TRIM46-decorated microtubules, in an EB-dependent manner.
1
0
4
We first developed knock-ins to label endogenous Nav1 channels, and used the AnkyrinG-GFP mouse line generated by
@jenkins_paul
and
@AxonalLab
. We were excited, because these tools could allow us to follow endogenous AIS major proteins during plasticity.
1
0
3
@christlet
@EMBOaxons2021
@AxonalLab
thanks!!! That's so cool to share science (well, almost as cool as sharing a meal, so bon appétit!)
0
0
3
@werner_hauke
@ewers_helge
@RaluucaG
@taraskalab
@DimovaLab
@cmatthaeus2
@MpiciPotsdam
@FUBiochemistry
@FU_Berlin
Wow, this is really cool! Congrats to the team!
1
0
2
To image the AIS of individual neurons during plasticity, we used endogenous labeling of (i) AnkyrinG using the cool mouse line shared by
@AxonalLab
and (ii) Nav1.2 channels using the ORANGE toolbox developed by
@Jelmerwillems
and
@hdmacgillavry
.
1
0
3
@adorrego_r
@ERC_Research
@FGA_lab
@amsterdamumc
@VUamsterdam
Thank you Ana! I propose a hashtag
#AISawesome
or something 😉
1
0
3
@maxadrian
@christlet
@Roy_Lab_Thinks
I also convinced Lukas Kapitein to join ;)
We actually missed you at the labouting this week end Max!
2
0
3
I find really exciting that plasticity at the AIS and synapses occurs in parallel and shares common molecular pathways. More work has to be done to fully understand the co-operation between synaptic and intrinsic plasticity and its functional consequences for network function!
1
0
3
@jenkins_paul
Congratulations!!!! It's really great and well deserved (I'm sure Ankyrins are happy too!!), I hope we get the chance to celebrate together soon! 🎉🍻
1
0
3
0
0
3
@frantecol
... and not "because they want to take care of their children" as heard this week in a meeting 🤦♀️
0
0
1
3. TRIM46-mediated microtubule organization is crucial for the proper trafficking to the proximal axon of other AIS proteins, such as Neurofascin-186. Once in the proximal axon, AnkyrinG allows for the stable accumulation of Neurofascin by locally preventing its endocytosis.
1
0
3
2. We show that TRIM46 only binds to parallel microtubules and acts as a strong rescue factor. This way, it confers uniform orientation and stability to the microtubules recruited at the membrane by AnkyrinG.
1
0
3
@AxonalLab
@NoraJamann
Impressive work and very interesting story! Congrats
@NoraJamann
,
@AxonalLab
and collaborators.
0
0
3
@PirtaHotulainen
Very interesting commentary and paper! We report that the interaction between NF186 and AnkG opposes NF186 endocytosis at the AIS: . It would be interesting to assess if/ how AnkG and NuMA1 cooperate to block the internalization of NF186 and Nav at the AIS!
1
0
3
1
0
2
@PlanarPolarityT
@adorrego_r
Congrats to you! It’s a great story, I’m super happy I could be part of it!!
0
2
3
pepernoten compensate for the lost imaging session :D
0
0
3
Nice colloquium in Amsterdam on "Axonal Cell Biology" with very good speakers
@christlet
:)
In November, we are organizing a KNAW colloquium on "Axonal Cell Biology" in Amsterdam:
0
6
2
0
0
3
@KapiteinLukas
@UniUtrecht
Congratulations Lukas! Looking forward to more stunning images from microtubules in neurons!
1
0
2
@feline_lindhout
@christlet
@christlet
What's the legend?? Beer drinkers do not agree here!
#DNM18
@hdmacgillavry
@feline_lindhout
@NickyScheefhals
1
0
2
@christlet
“Wow trop stylé!!!” From my 7 yo! That’s how I got into research, and I clearly remember the first time I saw neurons stained for tubulin 😍 (looong time ago!) with
@peris_leticia
and
@MoutinMJ
0
0
2
Congrats
@Verstreken_Lab
!!
We're delighted that eLife Reviewing Editors Frank Jülicher and Patrik Verstreken have been elected as new
#EMBOMembers
, and that Senior Editor Tadatsugu Taniguchi is a new Associate Member too. Congratulations to all!
0
1
6
0
0
2