Pleased to share that the optogenetic microtubule severing tool #Optokatanin✂️ is now out in @CurrentBiology !!! https://t.co/mbRjV20ehr Movie shows opto-katanin being used to locally sever microtubules in two locations (marked by blue boxes) inside a neuron.

Radial microtubules in cyan, non-radial microtubules in yellow.

Pasolli, Meiring, Akhmanova et al. (Utrecht University) describe tools to acutely relocate #vimentin intermediate filaments by inducibly coupling them to microtubule motors. https://t.co/4yyCMASFp2 @psychescope @gijsjekoenderi1 #Technology #Cytoskeleton

Excited to share that our fast & reversible chemical and optogenetic #vimentin pulling tools are now published in JCB! 🥳 https://t.co/nj9KHUCYCS

Hi! I was wondering if anyone has successfully cultured happy HEK293T cells in defined serum alternatives? If so what did you use?

P.S: Please follow me on blue sky:

Excited to share our new pre-print showcasing tools (chemical & optogenetic) for repositioning #vimentin in cells! Movie shows #ER (Halo-KDEL) sheets being relocalized together with vimentin (Vim) upon local vimentin pulling in U2OS. @gijsjekoenderi1 https://t.co/3VsESprJVn

@ blue skies, there is https://t.co/zz7OjZ35rp a collection of 150 chemical biologist accounts to make it really easy to jump start your #ChemBio network. Why not at Twitter? :( no wonder people are leaving here.

When the blue dot is shown, blue light pulses are being locally applied inside the yellow circle, which induces the recruitment of the microtubule severase katanin p60 to microtubules. Upon p60 recruitment microtubules are locally disassembled and the ER network simplifies.

Please enjoy this movie of local #microtubule disassembly inside the yellow circle using #optokatanin (magenta) with ER labelled in cyan & please give me a follow on Bluesky! https://t.co/QcVDDbxYQa

.@JagerLeanne, @psirez et al. @Bijvoet_Centre show that compacted and expanded microtubule lattice spacings coexist within cells and that #microtubules recognized by a marker for stable microtubules are predominantly expanded. https://t.co/0KEnSIEYkJ

I'll be talking about manipulating the cytoskeleton with #photopharmaceuticals and #optogenetic tools at this Monash Advanced Microscopy webinar. Anyone can join (if you are awake at 3pm AEST)!

Caged Epothilones* now out in Angewandte: congrats Carina & all! https://t.co/hehjB9ujqe Stabilises #microtubule #cytoskeleton when and where you want. Does what you expect & nothing you don't Giving it away for bio: just mail *&more: see Fig S1! @angew_chem @photopharma_lit

A very elegant study by some of my colleagues on decrypting lysosomal protein trafficking! They combine synchronised transport (using the RUSH system) with APEX proximity labelling to map out the lysosomal protein trafficking pathway in neurons. 👏🏼 #POTATOMap 🥔🗺️

Nice example of actin retrograde flow in a gigantic lamellipodium. #cytoskeleton, #actin, #microscopy, #cell-motility, #science

Harriet Saunders, Cyntha van de Berg, et al. show how ciliary tip proteins cooperate to make slow growing #microtubules in their new preprint! Together they form a structure that looks like a champagne cork in a #microtubule 😍 https://t.co/RqYgxlpbAd

Optogenetic control of the actin cytoskeleton in a neuroblastoma cell. Light is on when the white dot is visible. #microscopy #cell_control #science #cells_are_dynamic

Hi science twitter 👋 Do you test your mouse secondaries? I am looking for a reliable source of mouse secondaries for IF that don't cross react with rat, any recommendations? #Microscopy Pictured: goat anti-mouse secondary binding mouse anti-GM130 (golgi) and rat anti-tubulin

We do try to combine orders and order in bulk where possible and we ofcourse try to reuse them in the department but we still have a massive excess. 🫤

In only one month we collected 35 ice packs in our department from orders! The vast majority of ice packs are only used once and we think this is a waste! 😥 Do any companies reuse them? What does your department do? @greenlabs_NL @thermofisher @abcam @biotechne @SanbioBV

Klara Jansen, @mkiwanski, @KapiteinLukas et al. @UniUtrecht introduce StableMARK (Stable Microtubule Associated Rigor-Kinesin), a live-cell marker to visualize stable #microtubules. https://t.co/AoGfNPzAa6 #Technology #Cytoskeleton