Jared Schrader
@schraderlab
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Enjoy all things related to RNA, RNPs, and biomolecular condensates. This account is inactive, find us on https://t.co/KiisxL66JW
Joined November 2009
Our lab is excited to share our latest preprint describing the BR-body proteome with huge help from @wschilders44 @JulieBiteenLab @ChampionNd and Elaine Zhu's lab @WayneStateEng @WSUBioSci
biorxiv.org
Bacterial RNP bodies (BR-bodies) are non membrane-bound structures that facilitate mRNA decay by concentrating mRNA substrates with RNase E and the associated RNA degradosome machinery. However, the...
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Stress Changes the Bacterial Biomolecular Condensate Material State and Shifts Function from mRNA Decay to Storage https://t.co/W1lpTBzW1L
#biorxiv_biophys
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Polyphosphate affects cytoplasmic and chromosomal dynamics in nitrogen-starved Pseudomonas aeruginosa | PNAS
pnas.org
Polyphosphate (polyP) synthesis is a ubiquitous stress and starvation response in bacteria. In diverse species, mutants unable to make polyP have a...
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A rapid inducible RNA decay system reveals fast mRNA decay in P-bodies:
nature.com
Nature Communications - Studying RNA decay remains a challenging task. Here, the authors present a technology that enables inducible rapid degradation of targeted mRNAs. Visualizing mRNA decay...
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A conserved morphogenetic module that controls complex bacterial cell shapes. https://t.co/AzjyCkr5Mi
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Genome-wide profiling of Hfq-bound RNAs reveals the iron-responsive small RNA RusT in Caulobacter crescentus | mBio
journals.asm.org
The conserved RNA-binding protein Hfq contributes significantly to the adaptation of bacteria to different environmental conditions. Hfq not only stabilizes associated sRNAs but also promotes...
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I’m excited to share my first postdoctoral work where we captured the first high resolution structure of the bacterial magnesium transporter MgtA! This project allowed me to learn cryo with @DoreenMatthies, molecular simulations with @AlexSodt and function with @storzlab.
Our preprint on magnesium transporter MgtA is out revealing a DIMERIC, not monomeric P-type ATPase if you are gentle! Fun collaboration between 5 amazing labs. https://t.co/Xsyc2X8qip
@RileeZeinert @AlexSodt @storzlab @NICHD_NIH @NIH @MPIbp #cryoem
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Congrats to former postdoc Felix Keber from our & @MartinWuhr lab on this fascinating paper using filtration experiments w mass spec to show that ubiquitous mesoscale condensate formation across >18% of the proteome. https://t.co/Xgo4D3Rg8O
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Our story on tracking the in vivo NusG whereabouts is on @MolecularCell! NusG rarely walks alone, enjoys 30S company, works out on anti-terminating and TT-coupling. True saga by brilliant @HAFEZELSAYYED, super support by @ojp25 @M_Stracy and Max Gottesman!
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Thrilled to finally announce our work on the selectivity of disordered protein condensation in @NatureChemistry. Great collaboration with @JeetainMittal Led by @rmwelles and @kandarp_sojitra. https://t.co/b9ZO0g0Af1. See related work by Brangwynne lab https://t.co/HiUDH8vl9v
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Excited to see beautiful manuscript led by @RanaUshnish & Kevin w @iAmAllAdhesion @M_T_Walls @javalosLAB & AZP out in @NatureChemistry (...after 5 mo. copyediting :( https://t.co/Zca8VdO0rJ Also see related co-submission by @buenoscience & @JeetainMittal
https://t.co/WBS4LwjZ8K
So excited to share our work: We combined expts and sims to figure out how oligomerization can drive condensate immiscibility! Surprisingly which IDR is oligomerized matters i.e. asymmetric! Only possible thanks to our interdisciplinary super team, glad to have been a part of it!
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Interested in the mechanisms driving subcellular organization in bacteria? The Vecchiarelli lab has a postdoc position available! https://t.co/0GW7kzcOu5 Plz RT!
docs.google.com
Postdoctoral Position in Subcellular Organization in Bacteria RECRUITMENT PERIOD Open date: Feb 15th, 2024 Last review date: March 31st, 2024 Final date: August 1st, 2024 After the review date,...
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Happy to see this one in the print 🙂 We describe how disordered prion-like domains can drive selective co-condensation of fusion oncoproteins with chromatin remodelers😎 Postdoc @richoodavis led this work with two talented grad students. 👏👏👏 https://t.co/6beIMD8AyL
nature.com
Nature Communications - Prion-like domains are intrinsically disordered regions found in many RNA- and DNA-binding proteins. Here, the authors show that these domains can drive sequence-specific...
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How does cellular stress affect #RNA? We sequence entire RNA molecules with @nanopore sequencing. We find 👉Global RNA decay mediated by XRN1, independent of poly(A) tail! 👉Decay absolutely depends on stress-granule formation! https://t.co/hwxlDxNuf1
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Great to participate in this led by @HymanLab, Simon Alberti, @JeetainMittal and w/ @AyalaLab1, @titus_franzmann probing how cysteine oxidation aggregates TDP-43 Intra-condensate demixing of TDP-43 inside stress granules generates pathological aggregates
biorxiv.org
Cytosolic aggregation of the nuclear protein TDP-43 is associated with many neurodegenerative diseases, but the triggers for TDP-43 aggregation are still debated. Here, we demonstrate that TDP-43...
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This is a very exciting paper with one of the clearest applications so far to take advantage of bacterial single-cell RNA-seq's ability to pull apart heterogenous gene expression within populations. Highly recommend!
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We have a new story out at BiorXiv! Amazing work led by @za_curtis (Bisson), Pedro Escudeiro (Alva) and John Mallon (Bisson). Great collaboration with @Vikram_Alva (Max Planck) and @pohlschr (UPenn). A summary of our findings in the thread (1/22) https://t.co/qGvuQ7mwO1
biorxiv.org
Bactofilins are rigid, non-polar bacterial cytoskeletal filaments that link cellular processes to specific curvatures of the cytoplasmic membrane. Although homologs of bactofilins have been identif...
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What determines polar mRNA localization in E. coli? Shanmugapriya Kannaiah, @OChoder et al @Hujimed find the polar transcriptome to depend on MinD inhibition of RnaseE recruitment https://t.co/eTpUHranIy
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In a new paper in @Nature, an impressive and creative series of insights led @DDunkelmann and coworkers in Jason Chin’s lab to develop a powerful, general in vitro selection for aminoacyl-tRNA synthetase enzymes that charge tRNAs with a wide variety of small molecules, including
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Late consolidation of rRNA structure during co-transcriptional assembly in E. coli by time-resolved DMS footprinting https://t.co/IS9JhE2a7G
#bioRxiv
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Anyone able to recommend vertical PAGE rigs for preparative RNA purification after T7 transcription? I inherited all my PhD Adviser Olke Uhlenbeck's old gel rigs when he retired, but they were all custom built, so I need to find commercially available replacements.
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