Excited my PhD work is out now @NatureBiotech! We report Multiplexed Orthogonal Base Editor (MOBE) systems: tools to simultaneously install distinct point mutations. MOBEs introduce co-occurring genetic variants (polygenic disease, haplotypes)🧬 🧵⬇️ 1/

Check out MitoScribe, our new preprint led by @Linhan_W: https://t.co/rvNOSaMily... It's an analog molecular recorder that uses neutral base edits to the mitochondrial genome to store info about historical signaling in a cell. Single cell resolution at scale (see next post)!

1/10 Today in @ScienceMagazine in collaboration with @liugroup we report the development of a laboratory-evolved CRISPR-associated transposase (evoCAST) that supports therapeutically relevant levels of RNA-programmable gene insertion in human cells. https://t.co/y2DqXZzY1R

The Conklin Lab is hiring! We are seeking a Research Associate I to support ongoing projects focused on DNA repair and genome editing, particularly using motor neurons as a model system. Apply Here: https://t.co/30Ct9hQ3xL

Circuit not working? Time to check your syntax... #synbio @MITChemE @MITdeptofBE

Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations https://t.co/pKEjQMa9XX

Today we report Evo, a generalist foundation model for biology, on the cover of @ScienceMagazine. Trained on billions of DNA nucleotides, Evo was designed to capture two key aspects of biology: the multi-modality of the central dogma and the multi-scale nature of evolution.

Excited to share our new system for evolving eVLPs with improved production and protein delivery efficiencies! Grateful to @davidrliu and @liugroup for support throughout this study, and special thanks to co-authors Meirui An and @paulchenz for their contributions!

Attention gene editors! We are searching for faculty in gene editing (broadly defined) at UC Irvine:

So you want to engineer your hiPSCs, but targeting DNA payloads requires multiple slow, inefficient steps for each construct. What if we could accomplish multi-site integration seamlessly? 🚨Introducing STRAIGHT-IN Dual! 🧵 (1/n) Link: https://t.co/4BKAf4Or90

*Efficient PAMless base editing in patient-derived HSPCs* 🧬🖌️ https://t.co/UwjoM3u1yK For a few years now, we've been collaborating with clinical experts to develop genome editing-based treatments for genetic diseases. It has been a great fortune to work with, and learn from,

Our latest paper is out! In collaboration with @DrAnneCarpenter, we conducted a systematic high-content screen to understand the role of protein mislocalization in diverse human disorders. 1/

Saisha loves chess and singing. She is also affected by Charcot-Marie-Tooth disease, a genetic neuropathy that causes progressive damage to nerves in her legs and arms. In 2023, Saisha and her parents visited Gladstone. Read more: https://t.co/AWU5P4mbIT

Genome editing with DNA-dependent polymerases #NBTNV https://t.co/TGkhF2H9Bs

Check out our latest study on the DNA polymerase theta (Polθ), a precision oncology target for HDR-deficient cancers. Our work uncovers the inhibitory mechanism of a potent Polθ inhibitor using cryoEM, accelerating rational drug development strategies: https://t.co/ctvAprYhAI

Having a hard time with #CRISPR HDR? Your cells might be fighting against you! TREX1 nuclease, high in some cells, eats up HDR donor templates. Check out our paper in @NatureBiotech for the story & ways to improve HDR. https://t.co/03GokR65LA With @karasuerman1 & @J_Maciejowski

Excited to share 'frugal' CRISPRkit, a gene editing tool for K-12 education! Developed almost entirely by high school and undergrad students, led by Marvin & Matthew, this kit brings hands-on CRISPR experiments to classrooms to explore genome engineering without equipment. (1/2)

Research Highlight by @madhuramukho: Multiplexed Orthogonal Base Editor (MOBE) systems developed by @komorlab, @quinn_cowan_sci & colleagues is a suite of tools that use RNA aptamer systems to enable simultaneous distinct point mutations at different loci. https://t.co/d5pswLEUfK

Check out this great summary of our work "MOBE: A Base Editor That Multitasks without Mix-ups" by @TheScientistLLC @aparnanathan featuring @sahakris @KomorLab 🧑‍🔬🧪@UCSDChemBiochem @UCSDPhySci

Thank you @naturemethods @madhuramukho for highlighting our research: Development of Multiplexed Orthogonal Base Editor (MOBE) systems! 🧬@KomorLab @UCSDChemBiochem @UCSDPhySci

So excited to share one of the final works from my PhD in @SethShipman’s lab with @Asim_g_Khan and @oso_de_antojos, where we used high-throughput libraries and machine learning to create design rules for retron gene editors: