Plasmid crosstalk--unexpected changes to protein expression levels due to interactions between genetic cassettes in cell-free systems--complicates the creation of multi-plasmid cell-free tools. While...

Microbial bioprocesses are associated with environmental advantages, but their freshwater demand remains a challenge. Seawater is abundant and a sustainable alternative; however, high salinity often...

Brewer's yeast (Saccharomyces cerevisiae) acquires nitrogen from branched-chain and aromatic amino acids via the Ehrlich pathway, generating flavor (fusel) byproducts. Recently, diverting 4-hydroxy...

QS-21, a saponin extract from the Chilean tree Quillaja saponaria, is gaining popularity as a potent vaccine adjuvant, but its production is constrained due to its low natural abundance and complex...

Predicting how DNA sequence specifies gene expression remains a core challenge across regulatory genomics. Most predictive assays and models depend on native genomic DNA, constraining the full...

Overlapping genes allow multiple proteins to be encoded from a single DNA sequence, including convergent (antisense; tail-to-tail) orientations across three reading frames (phases 0, 1, and 2), with...

Engineering native gut bacteria offers a route to persistent, programmable therapeutics, yet many dominant taxa remain genetically intractable. Lachnospiraceae are a prevalent and abundant family in...

The American chestnut (Castanea dentata) was a foundational forest canopy species in eastern North America until an imported fungal blight (caused by Cryphonectria parasitica) rendered it functiona...

Protein design has been transformed by deep generative models ,but at the cost of interpretability, accessibility, and integration with sparse experimental feedback. Here, we introduce Genomic...

Implementing logic functions in living cells is a fundamental area of interest among synthetic biologists. The goal of designing biochemical circuits in synthetic biology is to make modular and...

Using genetic code expansion, canonical amino acid residues can be site-specifically substituted by non-canonical amino acids (ncAAs) with modified chemical properties. This technique has enabled...

3-Hydroxy fatty acids (3-HFAs) are versatile intermediates for bio-based polymers, fuels, and surfactants, and are advancing circular economy manufacturing. We built an acyl– CoA ligase-deficient...

Rhodobacter sphaeroides is a purple non-sulphur alphaproteobacterium with a highly versatile metabolism. This microorganism holds promise as a chassis for sustainable biomanufacturing of numerous...

Diatoms are globally significant microalgae that contribute ∼ 20% of oxygen production and exhibit remarkable metabolic diversity. The marine diatom Phaeodactylum tricornutum has emerged as a...

Genetic code expansion with non-canonical amino acids (ncAAs) opens new opportunities for the function and design of proteins by broadening their chemical repertoire. Unfortunately, ncAA incorporat...

The lack of robust genetic tools has hindered the precise engineering of Zymomonas mobilis , a key organism for the bioproduction of many commodity chemicals. Here, we developed a library of synthe...

Characterizing mutation effects on protein-protein interactions (PPIs) is crucial for elucidating protein structure and function. Massively parallel PPI variant analyses such as deep mutational...

Engineered microbial cells can produce a wide range of industrially relevant chemicals such as pharmaceuticals, fuels, and material precursors. The use of microbial cells for chemical production from...