Katie Galloway
@GallowayLabMIT
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Assistant Prof @MITChemE ; mom^4 + wife; enjoys building cell-fate circuits, exploring dna topology, reprogramming the living world, and soccer; soli gloria deo
Joined June 2019
Delighted to bring gene circuits into a wide array of primary cells where they can be used for safe, effective gene therapies. š§µ (1/n). Tremendous work by lead author @kaseyslove to dig into the details of these systems and figure out how to get these to work well!. Original
Loss of gene expression can compromise cellular fitness. In the case of such haploinsufficiency, replacement of the gene supplementation could rescue cell health. But what if you need a very precise dosage? Try ComMAND! š§µ (1/n).Link:
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RT @Mayr_Christine: More than 2700 3ā²UTRs are highly conserved. These 3ā²UTRs are essential components in mRNA templates, as their deletionā¦.
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RT @RosanaFerrero: š La regresiĆ³n lineal parece sencillaā¦ hasta que deja de serlo. š„.ĀæEstĆ”s seguro de que la estĆ”s aplicando bien? . AquĆ vā¦.
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until you defEND.
boyfriEND. girlfriEND. bestfriEND. you know what doesn't end? A PhD.
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senior grad student plasmid prepping for fig S36F.
Humans can get so good at weird manual dexterity shit this why biotech R&D is hard to automate
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Nifty! This can solve a lot of challenges we see with DNA delivery! Nice work @GraceKC!.
Can we use RNA to insert kilobases of DNA into the human genome, opening up a new way to engineer cells or treat disease?. Excited to share our latest work turning a retrotransposon (a ājumping geneā with an RNA intermediate) from songbirds into a genome editing tool. š§µ (1/7)
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RT @SRouhanifard: New from the Rouhanifard Lab!.What if we could sort single cells based on whether a specific gene was actively being tranā¦.
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RT @stemcellreports: The ISSCR has selected five distinguished early career scientists to serve as new Early Career Editors for Stem Cell Rā¦.
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RT @claudiaevickers: Poster abstract submissions for #SBA2025 are closing on 18th July - get yours in now! See you in Wellington in Novembeā¦.
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Watching people argue about models or experiments as essential features in biology is like watching people argue if a car should have brakes or a gas pedal. You probably drive most effectively if you can use both.
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RT @wxkylegillett: probably one of the cooler boundary collisions this year just now in the TX Panhandle #TXwx
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Kid 1: who's the best actor? Jim Carrey?.Kid 2: what about Charles Dickens?.Me: Dickens is a writer. Kid 3: Dickens isn't a writer. Me: Yes, he is. Kid 3: No, He's dead. His profession is being dead. He has years of experience. He has tenure. šš.
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RT @taekjip: high-throughput single-molecule platform to study DNA supercoiling effect on proteināDNA interactions .
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Ah, here is the working link to the paper. Not sure why the original link doesn't work.
So you want to change transgene expression: just change your promoter, right? Changing the promoter increases RNA and thus protein levels. What more could be happening?. [1/n] Well, promoters donāt just set RNA levels; they uniquely transform how RNAs are transmitted into protein
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[38/n] Now that we can see how promoters are affecting expression of transgenes, we have an enormous opportunity to improve forward design of gene circuits for applications across mammalian cells from biomanufacturing to precision cell and gene therapies. Excelsior!.
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[37/n] Weāre grateful for our funding sources that include NIH, NSF, AFOSR. This project really began with a very fundamental question but the applications and insights offer us new paths to translating synthetic biology into cell and gene therapy spaces as well as for our.
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[36/n] Weāre excited for next steps with the new understanding of these systems. While we saw that the UTRs we used had little effect, there are many variants that may have much stronger effects. Now that we have defined the TSS, combining novel 5āUTRs is now much simpler across.
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