Ivano Legnini
@ILegnini
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Group Leader @humantechnopole . Postdoc @BIMSB_MDC @MDC_Berlin . PhD @SapienzaRoma . Molecular & Systems Biology. RNA enthusiast.
Joined August 2018
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1/6 📣 Postdoc and PhD positions in my lab!
I’m looking for talented postdocs & PhD students passionate about gene regulation and RNA metabolism in neurons & neurodevelopment to join my group
@humantechnopole
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I'm looking for an intern! 🐣
If you just finished your master or are about to and want to do a 6 months+ project working with
#organoids
,
#optogenetics
,
#singlecell
and
#spatial
omics, DM or drop an email in the next couple of weeks
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Still about a week to apply for a postdoc in my lab! Also PhD positions open. We love all things
#RNA
,
#neurons
and
#organoids
, cool techs like
#longreads
,
#optogenetics
and much more. Bonus: the food, the sun, the mountains and wonderful people in the lab & institute :)
1/6 📣 Postdoc and PhD positions in my lab!
I’m looking for talented postdocs & PhD students passionate about gene regulation and RNA metabolism in neurons & neurodevelopment to join my group
@humantechnopole
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244
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1/8 We are hiring!!! 🐣🐣🐣
Fully funded PhD position in my group!
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Most epic farewell I could dream. Thank you
@BIMSB_MDC
for these wonderful years. A big piece of my heart will always be in Berlin.
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Kick-off meeting with my first lab members
@humantechnopole
@zapzap87
@LindaMasante
and we already found a ping pong table on campus. It’s a good day
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1/10. Our
#FLAMseq
paper is finally out on
@naturemethods
!
If you’re interested in
#RNA
#polyAtails
,
#generegulation
and
#longreads
with
#PacBio
, this thread is for you.
The paper is here:
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Is it still postdoc appreciation week? I just want to brag about the wonderful postdoc I had the luck to get on board.
@LindaMasante
thank you for your incredible passion and dedication, you will do great things
@humantechnopole
#NPAW2023
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My favorite day of the year. Fuck fascism for the 75th time, and always.
#BellaCiao
Interrompo i miei festeggiamenti casalinghi per condividere con voi la celebrazione del 25 aprile in una casa di riposo carpigiana ❤️
Viva la resistenza! Viva la memoria storica dei nostri nonni che è la nostra libertà
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My first effort in the
@N_Rajewsky
lab now on
@biorxivpreprint
: full-length mRNA and poly(A) tail sequencing with FLAM-seq
@BIMSB_MDC
@MDC_Berlin
@EMBO
#pacbio
#rnaseq
#longreads
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Wow, I couldn’t have written a better ad.. 2 postdoc and 1 technician position in my lab
@humantechnopole
- see thread below!
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Check out our latest work on a lung tumor 3D molecular atlas led by awesome
@TMPentimalli
@N_Rajewsky
&lab
@nu_karaiskos
@danilexn
@MDC_Berlin
@BIMSB_MDC
with
@FKlauschen
@CosciaLab
SPiccolo AWoehler
@nanostringtech
@AIgnostics
and many others. Bravo Tancredi! (and everyone :)
Cells live and interact in 3D tissues but pathology and spatial omics typically rely on 2D sections...
Excited to share our latest efforts in
@N_Rajewsky
lab to generate a 3D, multimodal atlas of a human tumor!
Find out what we learned: video + 🧵!
🔗
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Neurodegeneration in HSV1-infected brain organoids: our latest effort led by Agnieszka Rybak-Wolf and
@ewyler
+ many other amazing colleagues from
@N_Rajewsky
,
@landthaler_m
and Woehler labs
@BIMSB_MDC
@MDC_Berlin
out in
@biorxivpreprint
! Check it out :)
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Out now in
@CellCellPress
!!! Super exciting collaboration led by Valérie
@hilgers_lab
, especially the awesome
@extended_gene
and many others. Always amazed by how much we still need to understand about transcript architecture, RNA metabolism & so on - so much to learn still!
Where transcription begins determines where it ends! Check out our latest study, published today in
@CellCellPress
. A huge tour de force, led by the extraordinary Carlos Alfonso-Gonzalez
@extended_gene
🧵1/10
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Still 10 days to apply for 2 postdoc + 1 TA position in my lab
@humantechnopole
! Topics: gene regulation, RNA metabolism, neurodevelopment, optogenetics+organoids, single-cell/spatial omics. Background in physics or math also more than welcome. DM me if interested! Pls RT 🧡
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Join virtually this Friday to hear about awesome PhD projects at
@humantechnopole
Positions in my lab too! 🐓🐣🐥
📢 Don’t miss the Student Virtual Open Day organised by Human Technopole!
🔬 University Students and young scientists are welcome to join our Group Leaders to explore internships and PhD projects available in our Research Centres.
Register now 👉
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Fantastic start at
@KeystoneSymp
Single Cell 2022 with
@Eileen_Furlong
keynote. It’s so good to be back in person, thank you so much
@LabPagani
and
@N_Rajewsky
for making this happen. In beautiful Florence nonetheless
#KSSingleCell22
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6/6 Bonus: awesome food, lots of sun, mountains and lakes close by, and we grow tomatoes❤️. Oh, and we have ping pong!🏓🏓🏓
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Still a month to register to Brainomics2.0!
A week of theoretical and hands-on sessions to learn
#singlecell
and
#spatialomics
analyses for brain research
@humantechnopole
in Milan.
Incredible line up of speakers and instructors, see & register here:
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Terrific work, huge congrats Marie, Daniel, Elena, Pino, Nikos, Nikolaus and everyone involved!! Likely a game changer for many labs doing spatial omics 🚀🚀🚀
Our
#OpenST
paper is now online
@CellCellPress
!
Very cool updates ahead🧵⬇️
@L_Marie_Schott
@_Elena_93
#GiuseppeMacino
@nu_karaiskos
@N_Rajewsky
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🚀🚀🚀 Still a couple of weeks to apply for a
#postdoc
position in RNA biology of B cells
@humantechnopole
!!!
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What a fantastic journey this was. Thank you
@zolotarg
(and all other authors!), you are a gem and I can only wish to work with you again. Very important for me: we show that
@PacBio
Iso-Seq + our FLAM-seq is a great combo to annotate transcriptomes. More will come on this :)
Our story about small RNAs in big brains 🐙🧠 is now out in
@ScienceAdvances
.
A long 🧵 below ( grab a coffee!) :
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I knew very little about B cells but now my whiteboard is filled with Blagoje’s drawings. His passion is inspiring and this position is an awesome opportunity, don’t miss!
📣Postdoc position in my lab
@humantechnopole
. If you are passionate about immunology and B cell biology, happy to have a chat!
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If you're interested in
#longreadsequencing
of
#mRNAs
and
#polyAtails
come to my talk, the first in session 10 on new research trends and technologies, at 2.30 in the theatre hall!
#FLAMseq
#RNA2019
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1/5. By looking at nascent RNA in different ways, we find that the length of poly(A) tails at synthesis has a broad distribution peaking at 200-250 nt, much longer than that at steady state.
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Bonus credits: all scientific images in this thread are from the incredible work of our lab chancellor
@ZappuloAle
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We will also open PhD positions in a few months, this might be a great opportunity to explore our lab and projects before applying for a PhD
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Great work Grisha & team. Octopus rules!
From my side, really happy to see how FLAM-seq combined with Iso-seq can massively help annotating the transcriptome of non-model species. Let's do this again :)
So happy to see my internship project at
@N_Rajewsky
@MDC_Berlin
out in
@biorxivpreprint
! Great collaboration with
@BastianFromm
@ILegnini
@gianlupol
@EveSeuntjens
@Ruth_Styfhals
and other amazing colleagues without tw.
We have found something remarkable.
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Bonus 2. Just back from the first one and apparently we have fantastic lab retreats. First photo credit
@monika_piwecka
, our special guest along with
@harschnitz
🧡
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🔥 🔥 🔥
It's that time of year!! 🎉We are so excited to announce our 2023 pick for Nature Methods' Method of the Year: methods for modeling development. Read the Editorial to find out why we chose this as our pick.
#moty2023
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A unique course with an incredible list of speakers and instructors, join us in November!
🧠Give a twist to your neuroscience with
#BrainOmics
2.0, a unique interdisciplinary hands-on course on single-cell multi-omics computational analysis with integrative sessions on functional genomics.
🎯Only 20 seats for this 2nd edition! Apply now!👉
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Join us for a 4 years fully funded postdoc position. Outstanding scientific environment and infrastructure, competitive contract, super fun crowd and the perks of living in beautiful Milan :)
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Amarena was a sweet and harmless mother bear. We had already lost Juan Carrito, her son, symbol of joy and freedom and love for biscuits. This is beyond comprehension. Pnalm and the whole Abruzzo should react strongly to the culture that brought this disaster upon us.
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Two key spatial omics events this fall! 🔥🔥🔥
Spatial Biology Symposium
@humantechnopole
Milan and
@EMBO
#EMBOspatialOmics
in Rome.
Bonus for November: BrainOmics 2.0 course for single cell/spatial omics applied to neurobiology, all absolutely not to miss.
Links below 👇
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@robersperanza
fondamentale che l'Italia non perda questo treno e si faccia promotrice in UE di
@LifeTimeIni
iniziativa importante coordinata da
@N_Rajewsky
@GAlmouzni
@MDC_Berlin
@institut_curie
esce oggi su
@nature
, con tant* scienziat* italian* a bordo
@gtesta72
Ambitious perspective for
#Europe
:
#LifeTimeInitiative
& improving
#European
#healthcare
through cell-based
#interceptivemedicine
Out in
@Nature
today!!!
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Copy/paste your favourite GEO entry and get results in 4 minutes. Pretty amazing RNAseq analysis resource by the Ma'ayan group
#BioJupies
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8/8 ...located in beautiful Milan, incredibly exciting city with its amazing culture and food scene, and wonderful nature around.
Write me if you're interested or for any questions, and please RT ❤️
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“A comment more than a question: I love this red dot that keeps moving on the screen”
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Absolutely recommended!
2 weeks left to apply for a postdoc position in our lab
@HumanTechnopole
(
@NEUROCOV_eu
).
You will be part of a collaborative team & receive extensive support & mentorship to develop your own independent line of research.
#stemcells
#virology
Please RT
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“Ma il problema siete voi che vedete fascisti ovunque” 🤡🐀🙃
In
#GioventuMeloniana
, la nuova inchiesta di Backstair per
@fanpage
, emerge il lato oscuro di Gioventù Nazionale, il movimento giovanile di Fratelli d’Italia.
#fanpage
#backstair
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Super collaborative effort led by amazing
@RybakWolf
@ewyler
@TMPentimalli
with
@_annaoliveras_
and many others in
@N_Rajewsky
@landthaler_m
@ATWoehler
labs.
Very happy to see our paper on HSV-1 infections in brain organoids out in
@NatureMicrobiol
!
Lead by
@RybakWolf
, with
@TMPentimalli
@ILegnini
@_annaoliveras_
@landthaler_m
@N_Rajewsky
@MDC_Berlin
@BIMSB_MDC
– summary in the thread below. (1/9)
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@AdrianoAguzzi
@CarmeloFerrai
I would probably infect iPSCs and generate cerebral organoids. Readout would be scRNAseq to infer cell types that are expanded or depleted in response to a certain TF over expression or KD. A sort of functional lineage tracing
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Diffraction patterns on the facade: it seems the sun is shining again on Berlin
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3/6 🔬 Our group combines molecular and systems biology to study
#generegulation
. We focus on transcriptome regulation in neurodevelopment/neurons, leveraging innovative techniques like
#longreadsequencing
,
#singlecell
&
#spatialtranscriptomics
,
#optogenetics
and
#organoids
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We discovered that poly(A) tail length is broadly dependent on 3'UTR and also on promoter choice. Moreover, we see quite some cytosines within the tails!
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Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes
1
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4/6 👨🔬👩🔬 We welcome candidates with diverse backgrounds in
#biology
,
#computational
biology,
#biophysics
,
#biochemistry
, and related disciplines. We aim at an interdisciplinary group, with everyone trying to develop at least a bit of both experimental & computational skills
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Super exciting start of the
#LifeTime
opening. To the (so far excellent) speakers: please spend more time to properly acknowledge the people in your labs that contributed to the amazing science you show!
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Thanks
@RNASociety
for the amazing
#RNA2019
meeting: I met old friends and new people, learned a lot, and had the chance to talk about my work. Thanks
#Krakow
for the amazing food and the wściekly pies. Now enjoying the smell of jasmine along the river, this is a beautiful city!
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@SouravDas_3D
@humantechnopole
You can find all the application details for both kinds of position here:
For postdoc
For PhD
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🔥🔥🔥
@translatomics
Monitoring mRNA dynamics after DDX3X depletion indicates translation suppression followed by mRNA stabilization, with GC content in the CDS being a strong predictor of mRNA stabilization ➡️
@translatomics
@CalvielloLab
@humantechnopole
#RNAbiology
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And this year makes it two. Happy birthday Rome, my second home. MMDCCLXXIII ab Urbe condita.
First easter in my beloved Berlin, but I’ll
never forget you. Happy birthday Roma. MMDCCLXXII.
#NatalediRoma
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Giorgio Parisi ❤️
BREAKING NEWS:
The Royal Swedish Academy of Sciences has decided to award the 2021
#NobelPrize
in Physics to Syukuro Manabe, Klaus Hasselmann and Giorgio Parisi “for groundbreaking contributions to our understanding of complex physical systems.”
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5/10. We sequenced HeLa, iPSCs, brain organoids, C. elegans. Each biological sample has its peculiar poly(A) length distribution, and tail length varies from gene to gene, within approx. 25 and 250 nt. 50 nt seems a special length: all mRNAs have a peak around there.
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La ricerca in Italia è messa male, ma siamo sicuri di voler imitare gli altri paesi? Qualche spunto di riflessione su Berlino Magazine
@BerlinoCP
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We want to understand how splicing, polyadenylation and translation are tuned to sustain B cell differentiation and antibody secretion
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6/8 ...and we will use single-cell and spatial omics to study how cells respond to perturbations in space and time
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2/6 😏 Why
@humantechnopole
? Located in beautiful
#Milan
, HT offers a vibrant and collaborative research environment. Join a community of brilliant scientists from diverse disciplines and backgrounds to work together on many aspects of human
#biology
.
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Meravigliosa, anche dopo tanto tempo
Una versione mozzafiato di "Nuotando nell'aria" dei
@marlenekuntz
eseguita in solo da Cristiano Godano nel salotto di
@redazioneRockit
.
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First easter in my beloved Berlin, but I’ll
never forget you. Happy birthday Roma. MMDCCLXXII.
#NatalediRoma
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E ora aspettiamo che il capo del governo se ne assuma “la responsabilità politica, morale, storica”
In
#GioventuMeloniana
, la nuova inchiesta di Backstair per
@fanpage
, emerge il lato oscuro di Gioventù Nazionale, il movimento giovanile di Fratelli d’Italia.
#fanpage
#backstair
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ouch
Multi-omic measurements of heterogeneity in HeLa cells across laboratories
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5/8 We use optogenetics to locally perturb morphogen expression
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10/10. Finally, a short behind-the-paper blog post here:
I wish happy FLAM-seq runs to all!
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Wait, what?
Serotonin goes NUCLEAR!! Beyond excited to finally share almost 5 years worth of work. This was an enormous feat, with stellar collaborators
@nature
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And that whether an antibody is secreted or not is controlled by alternative splicing and polyadenylation?
As an RNA person who never worked in immunology, when
@BlagojeSoskic
told me about this stuff I was blown away
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