The interplay between poised RNA polymerase II & Polycomb on repressed developmental genes is maintained throughout terminal neuronal differentiation.

We are now on BlueSky too! https://t.co/GXqT8zM1xY

We are recruiting 👇 #PhD #INPST

Sonoepigenetic Modification Mechanoprimes Early Osteogenic Commitment in Mesenchymal Stem Cells

Spatial multi-omics reveals cell-type-specific nuclear compartments | Nature

We’re excited to see many of you in Paris next week! If you can’t make it in person, don’t worry—follow us here and on our website for access to the live stream. #laminopathies #lmna #paris #livestream

As widely stated, this of course is A hyped misrepresentation of the achievement. Not good for Science. We have to bear this type of false statement in #HumanEmbryo research; a famous example https://t.co/NRlycm9Qgu Important to push back & don't allow this to become a norm

https://t.co/q0a6HVDR9i

We are looking for a passionate computational postdoc to join our lab @humantechnopole in Milan! 🇮🇹🧬 Project funded by @AIRC_it to model the dynamics of cell therapies in blood cancers using single-cell multiomics 🔬 4-year contract https://t.co/bonFRXyTt5

I would like to congratulate with these amazing collaborators Lizebona Ambattu, Blanca del Rosal, Leslie Yeo it was a real pleasure to work together I hope that the scientific community will like the story! All the best 👋🏽 -END-

I believe we provide some interesting evidences on how extracellular cues target the nucleus to effect its fate and function in 2 ways. Nuclear shape and LaminA conformation rely on cytoscheletal action; chromatin fluctuations depend on two key second messengers:Ca2+and cAMP -9-

Importantly cAMP inhibition not only prevents the chromatin fluctuations but it affects the differentiation potential of the cells towards their Osteogenic commitment. -8-

as well as RNAPII-S5p and Coilin aggreagates -7-

In contrast we found that inhibiting cAMP specifically abolish chromatin fluctuation -6-

In order to understand the path by which mechanostimulation reaches/impacts the cell at nuclear level, we used specific drug inhibitors. Blocking actin polymerization block nuclear shape chanes and LaminA phenotype but not chromatin fluctuations. -5-

We observed a strong fluctuation also of the enhancer histone mark H3K27ac. Interestingly sonochallenge induces an enlargements of RNAPII-S5p nuclear foci like Coilin despite the fact that size and numbers of the foci do not seem coincide along the timecourse. -4-

At the chromatin level we noticed that sonochallenged cells increase the level of H3K27me3 and these results are paralleled by a decrease in their H3K9me3 distribution. -3-

Sono-stimulation induces an initial influx of Ca2+ via the Piezo Channel that eventually activate a wave of cAMP production. Cells response to sonostimulation show the formation of actin polymerization with pronounced stressfibers and wrinkling LaminA phenotype. -2-

I am Absolutely delighted to share the preprint of our last collaborative efforts where we investigated the impact of sonostimulation of Mesenchymal Stem Cells. Thread -1- https://t.co/Ha7PJ6EkTW

Dr. @CarmeloFerrai @uniGoettingen will present TOMORROW his research and ideas on how to apply #FluidFM and Live-seq in the field of #mechanobiology. @lorentzcenter

Only one week left to apply to join our lab at Imperial! Please do remember to send your applications on the Imperial website, even if you got in touch with me informally. I will only be able to assess formal applications and not other expressions of interest