Avery Roberts
@theCRISPRdrawer
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PhD from the @NCState @CRISPRlab | CRISPR-associated transposons & beyond 🧬
Raleigh, NC
Joined September 2019
Thanks to @ben_a_adler and @bradyfcress for their support on this, and to the rest of the m-CAFEs team and @CRISPRlab 🧬
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Happy to share that the final chapter from my PhD is now published! We engineered a phage λ mutant to remove its lysogenic potential and deliver an all-in-one CRISPR-associated transposon (CAST) system for targeted gene insertion within E. coli. https://t.co/ouT7mpASMv
pnas.org
Phage λ, a well-characterized temperate phage, has been recently leveraged for bacterial genome editing by selectively delivering base editors into...
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Our small discovery just got published 😇. Relatively few cyanobacterial genomes are home to most known families of CRISPR-Cas transposons (CAST). After a careful search, we found a new capture: type I-D CAST~ https://t.co/QyaBuNXGHb
@JoePetersLab
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I appreciate all the feedback for this work from #CRISPR2022! I'm looking forward to whatever comes next from the CRISPR-associated transposon research community...🧬🚕 (... and maybe it's a good time to check out Mastodon?) 6/
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Our findings align well with those of a recent study from @SanneKlompe @shsternberg (highly recommend a read: https://t.co/KAk66fTKFy). Based on that study's proposed TnsB compatibility groups, we experimentally validated that two new systems and Tn6677 are all orthogonal. 5/
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We also discovered rare instances of type I-F3 systems containing multiple self-targeting spacers in their native context. Unexpectedly, CRISPR repeats not associated with these self-targeting spacers resulted in the highest rates of integration. 4/
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We successfully reconstituted ten previously uncharacterized type I-F3 CRISPR-Cas systems for CRISPR RNA-guided DNA transposition. Overall, there was a wide range of integration efficiencies (~0.1-100%) and PAM requirements were exceptionally flexible (see Tn7479 👀). 3/
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For background, type I-F CRISPR-associated transposons are Tn7-like genetic elements mobilized by CRISPR-Cas + transposon machinery. In their native context, these systems are often >30 kb in size and harbor genes related to, for example, antiviral defense and metabolism. 2/
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Excited to share my first, first-author research article, with @CRISPRmatt @CRISPRchef, out now in @NAR_Open: https://t.co/W8egPnsPWp 1/
academic.oup.com
Abstract. CRISPR-Cas systems generally provide adaptive immunity in prokaryotes through RNA-guided degradation of foreign genetic elements like bacteriopha
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Latest paper from the lab showing how we can use CRISPR to manipulate the microbiome. @PNAS, https://t.co/HA9zCId86R,
pnas.org
Investigation of microbial gene function is essential to the elucidation of ecological roles and complex genetic interactions that take place in mi...
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Super excited to share my first publication with @doudna_lab @NatureMicrobiol : RNA-targeting CRISPR-Cas13 Provides Broad-spectrum Phage Immunity https://t.co/TGdpTk8f3u Now, with even more phage genome editing (and of course, CRISPR-Cas13) 1/
nature.com
Nature Microbiology - A CRISPR-Cas13 enzyme is shown to have potent anti-phage activity that is harnessed to produce a phage genome engineering method with broad utility.
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CRISPR 2022 conference was a blast, with lots of exciting new transposon biology! Fitting, to come just before #TransposonDay2022. We didn’t catch everyone for the pic but some key players @JoePetersLab @kellogg_liz @fthoffmann @MikePetassi …
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Heading back to @NCState after a great week in Boston for my first conference #crispr2022. Excited for the next wave of CAST papers!
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My entry for the @BioRender holiday card contest, ft. a cheerful Cas9 and rightly nervous (jumbo?) phage. #crispr #sciencegreetings
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Great job by @BasTeusink, @OscarKuipers1 ! Took us some time to finish this special issue, but awesome lineup of reviews ! Many thanks to all the authors and reviewers ! https://t.co/r3DgUyuSxR All content is made free for the next three months. @LABsymposiumorg @FEMSmicro
academic.oup.com
Lactic Acid Bacteria (Lactobacillales) are an order of diverse bacteria that play a vital role in food production and fermentation, probiotic products, and coul
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My first review with @CRISPRchef on CRISPR-Cas systems and LAB! The #CRISPR field moves quickly, with fascinating finds on what seems like a daily basis. (Shoutout to @BioRender for the fun figures)
#CRISPR-Cas systems can be useful for engineering #LacticAcidBacteria for new functions. Roberts and Barrangou cover #CRISPR-Cas classification, mechanisms as well as their current and potential applications in lactic acid bacteria. #FEMSMicrobiolRev
https://t.co/rB8Z6GcDRD
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BREAKING NEWS: The 2020 #NobelPrize in Chemistry has been awarded to Emmanuelle Charpentier and Jennifer A. Doudna “for the development of a method for genome editing.”
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Excited to announce the maiden voyage of the Modell lab, led by the outstanding @reworkman0 and @tejapammi. We found S. pyogenes Cas9 uses a natural single guide RNA, a conformation of the 171nt tracrRNA, to transcriptionally repress its own promoter. https://t.co/vYD5M1gFQF
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With @CRISPRchef, Spotlight on important recent work from @joeBondyDenomy and @PeterFineran. Eager to see what else the jumbophage have up their sleeves!
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New year, new lab photo! In 2019, we welcomed several new members: Natalia Cobian, Avery Roberts, MaryClaire Chamberlain, and Anna Garrell. We look forward to more exciting research in 2020 and hopefully more lab additions! @CRISPRchef @CRISPRmatt @theCRISPRdrawer @garrell_anna
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