Doudna Lab
@doudna_lab
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News from Jennifer Doudna's lab at @UCBerkeley @igisci . Tweets from lab members and not Jennifer Doudna unless signed JD. Tweets represent personal views only.
Berkeley, CA
Joined December 2015
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I am excited and a bit overwhelmed with emotion at the news of the approval of CASGEVY in the UK. Going from the lab to an approved
#CRISPR
therapy in just 11 years is a truly remarkable achievement. (1/3)
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A decade after we showed CRISPR systems could be used for programmable genome editing — our latest review is a look back at the work that has advanced this technology, and a look forward to the future of what may still be yet to come! 🧬✂️
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Brace yourself… More robust than RNAi and less toxic than Cas13… check out our latest story on CRISPR-Csm for precise RNA targeting!!
@NatureBiotech
@igisci
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We’re excited to announce the soft-launch of a new
#CRISPR
database, CasPEDIA (), a product of a large team co-led by
@ben_a_adler
and Marena Trinidad!
CasPEDIA summarizes and contextualizes enzymatic properties of widely used Cas enzymes:
1/
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Step aside Cas13 and RNAi! New Type III CRISPR-Csm tool for robust and precise RNA knockdown out on biorxiv today.
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Looking for the ideal CRISPR-Cas system for your experiment? CasPEDIA, our wiki of Cas nucleases, introduces a new functional nomenclature (CasID) to help you get started!
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We are looking for a lab assistant to work with us on CRISPR-Cas based editing of microbial communities:
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Wow! 50,000 followers! Thank you all for being curious about our research and always supporting our science. How long until we hit 100,000?..
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So proud of this effort
@igisci
to develop an amazing resource for both scientists and the general public!
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Doudna Lab past and present reunion after the International Conference on CRISPR Technologies 🧬🎃
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New Paper: Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy.
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I am especially pleased that the first
#CRISPR
therapy helps patients with sickle cell disease, a disease that has long been neglected by the medical establishment. This is a win for medicine and for health equity. (2/3)
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Come work with us! Seeking a Research Technician to work on new CRISPR gene editing tools. Job ID:
#56343
. Apply here -->
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Our CRISPR-Csm plasmids are now available through
@Addgene
Brace yourself… More robust than RNAi and less toxic than Cas13… check out our latest story on CRISPR-Csm for precise RNA targeting!!
@NatureBiotech
@igisci
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New Paper: Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity.
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Highlights from our Doudna Lab Sonoma retreat last week (a thread)!!
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A few days of much deserved time off during our lab retreat! We talked some science, drank plenty of wine, and explored beautiful Sonoma via hot air balloon, bike, safari Jeep, and horseback.
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...and the results!
Super proud to have played a small role in this work - a true multi-kingdom tale.
Side bar: watching
@PeterHYoon
make worms glow *with viruses* was a major highlight of 2022 for me.
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Today is an extraordinary moment for the field, for patients in need, and for the future. – JD (3/3)
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Come work with us to develop CRISPR-based diagnostics! Currently seeking a Staff Researcher Assistant III (9611C)
#16210
:
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New preprint from the lab: how RNA-guided nucleases🐛wormed 🪱 their way into eukaryotes. Proud of this collaborative effort and analysis led by
@PeterHYoon
,
@PetrSkopintsev
, and
@honglue1
!
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Welcome to the newest member of the Doudna Lab!!!
Please welcome baby boy Jude, born 12/30! A big thank you to all of the wonderful providers
@UCSF
who helped us through fertility, pregnancy, and newborn feeding and fussiness. Immensely grateful to experience the amazing care of my home department
@UCSFChildrens
@UCSFPediatrics
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Seeking a Research Technician to work with us on engineering CRISPR-Cas proteins:
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New Paper: A CRISPR-Cas9-integrase complex generates precise DNA fragments for genome integration
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CNS genome editing Reduced immunogenicity
🤝
Cell-penetrant Cas9 RNPs
Check out our new paper led by
@AbbyLabby_
with
@SavageCatsOnly
!
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I'm excited to be giving the keynote talk at
#CRISPR22
. Learn more at , and join me there!
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New Paper: Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
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Beautiful work from graduate student Carolyn Huang as well as
@ben_a_adler
characterizing, investigating, and harnessing the function of enigmatic Cas enzyme, Cas12c! Check out our new paper in
@MolecularCell
investigating nature's CRISPRi
@igisci
@berkeleyMCB
@QB3
"A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression" - Read more from this
@MolecularCell
paper here:
Carolyn J. Huang,
@ben_a_adler
,
@doudna_lab
@UCBerkeley
@BerkeleyLab
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We have another research technician open!!
Apply to come work in a diverse and dynamic laboratory environment
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Huge congrats to longtime collaborator
@BanfieldJill
!!! In good company with other microbiologists like Louis Pasteur, and the first woman to win the Leeuwenhoek Medal. So very well deserved🥂
@igisci
@UCBerkeley
Celebrating that Jill receives the Leeuwenhoek Medal this year! It is is granted every ten years to the scientist judged to have made the most significant contribution to microbiology during the preceding decade. Cheers
@BanfieldJill
!
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New paper on engineered Cas12a variants! Through both structure guided engineering and directed evolution we minimized trans activity, increased genome editing in human cells, and enhanced HDR efficiencies
@igisci
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New Paper: Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering
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New Paper: Launching a saliva-based SARS-CoV-2 surveillance testing program on a university campus
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Come join us in our efforts to develop precise DNA editing tools for microbiomes! We are hiring a lab assistant:
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Check out our latest preprint on CRISPR integration!! We found a natural protein fusion that coordinates spacer selection, processing, and integration as the first step of adaptive immunity
@igisci
@UCB_Chemistry
How do CRISPR immune systems make molecular memories? Joy, Petr and I characterized an all-in-one trimmer-integrase to ask questions about CRISPR adaptation. Really excited to share this one - my first preprint
@doudna_lab
@igisci
. Check it out!
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One final D-Lab 📸 from last week’s CRISPR Technologies Conference
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New Paper: CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells
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Exciting news -- congrats to Post-Doc Jenny Hamilton
@JRo_Hamilton
for being awarded a
@NIH
K99/R00 Pathway to Independence Award through
@NIGMS
!
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🧬🚨Now online at
@CellCellPress
from
@ConnorTsuchida
with collaborators at
@UCSF
,
@Stanford
,
@GladstoneInst
,
@Penn
, and
@parkerici
.
Systematically studying the phenomenon and mitigation of Cas9-induced chromosome loss in clinical human T cells!
1/2
Out today in
@CellCellPress
, a new paper from first author
@connortsuchida
of the
@doudna_lab
points the way towards making
#CRISPR
safer by avoiding chromosome loss. Read more:
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New Paper: Human Molecular Genetics and Genomics - Important Advances and Exciting Possibilities.
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New Paper: Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement.
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New Paper: Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics
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Lab + alumni happy hour! 🍻Great to hear what amazing things Doudna Lab alums are up to now
@stephenfloor
@MarcoLobba
@jamesknunez
@thecrispress
@kevdox
@BStaahl
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New Paper: Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex
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New Paper: DNA interference states of the hypercompact CRISPR-CasΦ effector
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New preprint from the lab co-led by
@AmyReginaEggers
and
@KaiChen_1990
!
iGeoCas9’s improved ability to rapidly unwind DNA is driven by 3 mutations. These mutations can be engineered into other Cas9’s to boost gene editing abilities! Check out
@AmyReginaEggers
post for details!
Today I am excited to share our preprint “Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9” from
@doudna_lab
, with co-authors
@kaichen_1990
, Kasia Soczek,
@owen_t_tuck
,
@Ee_Doherty
,
@BW_Thornton
, Bryant Xu, and Marena Trinidad.
1/7
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New Paper: CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells
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New Paper: CRISPR-CasΦ from huge phages is a hypercompact genome editor.
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Beautiful new review from the lab, written by soon-to-be lab alum Joy Wang and lab alum Patrick Pausch! Check it out if you want to learn about structural biology in all aspects of CRISPR
Hey
#CRISPR
friends! ✂️🧬 Joy Wang, myself and Jennifer
@Doudna_lab
wrote a
@NatureRevMicro
review on the ‘Structural biology of CRISPR-Cas immunity and genome editing enzymes’. (1/4)
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New Paper: CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells
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🎉 Big farewell to a lot of fantastic D-Lab members! We’re excited to see all the fantastic science you’ll achieve in your next steps 🧬
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New Paper: Accelerated RNA detection using tandem CRISPR nucleases
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New pre-print from the lab using non-viral genome editing in the mouse brain 🧠✂️🧬
Excited to share this pre-print on recent developments in non-viral delivery of CRISPR-Cas9 to the mouse brain, following up on work from the
@doudna_lab
published in 2017
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New Paper: Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement
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Out now – inspiring work from
@ben_a_adler
on how to edit the most abundant organisms on earth: bacteriophages. A tale of how one of the rarest CRISPR systems can tackle phage diversity and is a robust phage-editing tool.
More editing the Phage Genomic Universe coming soon!
Super excited to share my first publication with
@doudna_lab
@NatureMicrobiol
: RNA-targeting CRISPR-Cas13 Provides Broad-spectrum Phage Immunity
Now, with even more phage genome editing (and of course, CRISPR-Cas13)
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New pre-print led by Post-doc in the lab
@JRo_Hamilton
. Tackling one of the biggest challenges in therapeutic genome editing - cell specific delivery - with programmable enveloped delivery vehicles!
Check out our new programmable strategy for delivering genome editors to specific cell types - out today on bioRxiv 🎯
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Cryo-EM structure and molecular engineering of the compact and efficient CRISPR effector, CasX! Check out our new paper in
@MolecularCell
led by
@connortsuchida
@MSDoost
and
@GogoLiu
(now a Prof.
@Tsinghua_Uni
)
@igisci
@BerkeleyBioE
@berkeleyMCB
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New Paper: Species- and site-specific genome editing in complex bacterial communities
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New Paper: Programmed DNA destruction by miniature CRISPR-Cas14 enzymes.
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New Paper: Rapid assessment of SARS-CoV-2 evolved variants using virus-like particles
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Congrats to Doudna Lab grad students
@PeterHYoon
and Muntathar Al-Shimary on being awarded the
@NSF
GRFP!
@berkeleyMCB
@igisci
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A belated congrats to the winners of our 2022 D-Lab World Cup Bracket ⚽️
@evelyn_chen11
@lauraxhofman
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We're furiously typing here
@UCBerkeley
with
@igisci
to try and answer your questions on our
@reddit
AMA! Continue sending us your
#GeneEditing
questions!
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New Paper: DNA capture by a CRISPR-Cas9-guided adenine base editor.
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New Paper: Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs.
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Now in
@PNASNews
, using epigenetics to improve Cas9 HDR! Congrats
@evelyn_chen11
@enrique_lins
@MSDoost
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CRISPR, COVID, & point-of-care microfluidics! Check out our new paper in
@natBME
with
@pdhsu
@SavageCatsOnly
@FletcherLab_UCB
. Congrats to D-Lab members
@gavinjknott
,
@tinaliu_phd
, &
@Dylan_Smock
as well as all the other authors on this massive project.
New from
@doudna_lab
,
@pdhsu
, and
@SavageCatsOnly
: a sensitive
#CRISPR
-based diagnostic test for
#COVID
-19 with results in less than an hour – and no nose swab! 🚫👃 Learn more:
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New Paper: Robotic RNA extraction for SARS-CoV-2 surveillance using saliva samples
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New Paper: CasX enzymes comprise a distinct family of RNA-guided genome editors.
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New Paper: CRISPR-Cas guides the future of genetic engineering.
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🧬🚨 New preprint from
@ben_a_adler
and Muntathar Al-Shimary in co-led collaboration with
@bradyfcress
lab!
Catalytically deactivated Cas13 can inhibit diverse phages by mere RNA binding, enabling CRISPR interference in these fascinating viruses!
I’m really excited to share our new pre-print “Genome-wide Characterization of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi”, with co-first author Muntathar Al-Shimary
@doudna_lab
and the newly-minted
@bradyfcress
lab!
🧵🧵
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New Paper: The NIH Somatic Cell Genome Editing program
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New Paper: CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification.
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We're very excited to be hiring postdocs with Ben as part of the newly formed Precision Microbiome Editing Initiative!
Join the team developing the new field of
#microbiome
editing! We are recruiting a jointly advised postdoc with Jennifer Doudna
@doudna_lab
to apply new CRISPR-Cas systems to editing clinically and environmentally relevant microbiomes.
Learn more ⬇️
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Introducing BiomeForge, IGI's microbiome engineering initiative led by
@doudna_lab
@BanfieldLab
& new IGI Investigators
@EmeryRubin
@Dr__Diamond
@bradyfcress
! BiomeForge will use new
#CRISPR
-Cas systems in microbiomes to solve global challenges.
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Thankful for
@biorxivpreprint
! 62 days and still waiting for first round of reviews from journal 😕...
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New Paper: Massively parallel kinetic profiling of natural and engineered CRISPR nucleases.
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New Paper: Genome-resolved metagenomics reveals site-specific diversity of episymbiotic CPR bacteria and DPANN archaea in groundwater ecosystems.
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Watch out world! Former lab technician and newly coated Stanford PhD student
@CindySE27
is about to take the biosciences by storm.
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New Paper: Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules.
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New preprint 🚨 from
@connortsuchida
in our lab. We utilized multiple CRISPR screens to investigate Cas9-induced chromosome loss. Surprisingly, we found a clinical methodology to mitigate this cellular fate. Great collaboration with
@yimmieg
@HowardYChang
@carlhjune
@j_eyquem
Excited to share our latest story from
@doudna_lab
systematically studying the phenomenon and mitigation of Cas9-induced chromosome loss in clinical human T cells now on
@biorxivpreprint
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New Paper: Machine learning predicts new anti-CRISPR proteins.
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New preprint out on
@medrxivpreprint
: Accelerated RNA detection using tandem CRISPR nucleases
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Jennifer Doudna is partial to Osmium (Z=76), the densest naturally occurring element. Find out why!
#MyFaveElement
#IYPT2019
@BerkeleyLab
@igisci
@UCBerkeley
#CRISPR
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New Paper: Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing.
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New Paper: Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage
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New Paper: CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells
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New Paper: CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks.
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Congrats to our long time collaborator Eva Nogales and her lab for this incredible recognition! 🥂
A special day for our fearless leader. Congratulations to both Eva,
@CramerLab
, and all of the lab members who contributed toward our understanding of the molecular mechanisms of transcription initiation.
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New preprint out on
@biorxiv_micrbio
: Targeted Genome Editing of Bacteria Within Microbial Communities.
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New Paper: CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.
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New work from our lab
@abdullah_m_syed
and
@cychiu98
@TheOttLab
@GladstoneInst
investigates the neutralizing antibody response from various COVID variants. Check it out now in
@CellCellPress
!
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