Michael Booth
@DrMikeBooth
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Associate Prof of ChemBio+OrgChem @UCLChemistry @RoyalSociety URF chemistry, DNA/RNA, synthetic biology. Coeliac. Dad. 🎸
London, England
Joined April 2016
We have developed an entirely new mechanism of action for gene knockdown -> trafficking of targeted mRNAs to the lysosomal, using lysosomal trafficking oligonucleotides (LyTONs)! Published in @ChemicalScience Led by @disha_kashyap_, with @milnetom68
https://t.co/ciaFGwt5QG
pubs.rsc.org
Antisense oligonucleotides (ASOs) can modulate gene expression at the mRNA level, providing the ability to tackle conventionally undruggable targets and usher in an era of personalized medicine. A...
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We’ve developed the first-ever method to control nucleic acid activity and gene-expressing synthetic cells with a deeply tissue penetrating alternating magnetic field 🧬🧲! Published in @NatureChemistry Led by Ellen @OxICFM @SynCellEU @buildacell
https://t.co/bmoz5iL9Hg
nature.com
Nature Chemistry - The programmability of synthetic cells, comprising lipid vesicles that are capable of imitating the structure and function of living cells, facilitates their application as drug...
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We are pleased to share our review article with the Booth lab at UCL on synthetic cell communication, published in Nature Chemical Biology. https://t.co/3IdIhbleC3
nature.com
Nature Chemical Biology - Recent advances in engineering bottom-up synthetic cells have created powerful compartmentalized biochemical reactors with cell-like abilities. To push the boundaries of...
A new Review highlights state-of-the-art communication mechanisms between synthetic cells and discusses potential applications https://t.co/dWAHpiLDlD
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Our collaborative review article on the latest strategies and applications of synthetic cell communication is now out! With Allen Liu @UMengineering Published in Nature Chemical Biology @nchembio
@SynCellEU @buildacell @UCLChemistry
https://t.co/LccKvJzSMC
nature.com
Nature Chemical Biology - Recent advances in engineering bottom-up synthetic cells have created powerful compartmentalized biochemical reactors with cell-like abilities. To push the boundaries of...
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We have dramatically increased splice switching *and* RNase-H ASO activity by conjugating a nuclear importer! Published in @J_A_C_S Led by @disha_kashyap_, with @milnetom68 @NATA_MRC_UK
@chem_in_cells @OxfordChemistry @UCLChemistry
pubs.acs.org
Antisense oligonucleotides (ASOs) are a promising class of therapeutics designed to modulate gene expression. Both key mechanisms of action for ASOs operate in the nucleus: splice-switching ASOs...
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We have developed a dynamic ‘off’ switch for PROTAC activity by using DNA linkers and toehold mediated strand displacement! Published in @JACS_Au @ACSPublications Led by @disha_kashyap_, with @shozeb_haider + @milnetom68
@UCLChemistry @OxfordChemistry
https://t.co/nw1tDl8jsQ
pubs.acs.org
Targeted protein degradation is a powerful therapeutic approach: expanding the druggable proteome, providing enhanced selectivity, and having the ability to overcome conventional resistance mechani...
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We’ve integrated DNA nanotech into targeted protein degradation! Our oligoPROTACs act like small mol PROTACs but can be turned “OFF” — exciting alternative to pro-drug based activation for reducing toxicity! @DrMikeBooth @milnetom68
@chem_in_cells @OxfordChemistry @MRC_WIMM
New preprint 🎇 We developed oligonucleotide PROTACs (OligoPROTACs), which show comparable activity to small molecule equivalents - but can be *switched off*, using DNA nanotech, to restore protein levels! Last of @disha_kashyap_'s trilogy! @milnetom68
https://t.co/hW8lrV7ghb
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We have engineered lysosomal trafficking-ASOs (LyTONs)! Novel mechanism of gene knockdown🧬 LyTONs don’t need RNase H recruitment — work as well/better than state-of-the-art gapmers! #RNAtherapeutics
@DrMikeBooth @milnetom68
@chem_in_cells @OxfordChemistry @MRC_WIMM
🚨 Preprint alert 🚨 We have developed an entirely new mechanism of action for ASO gene knockdown -> lysosomal trafficking 🧬! Works with non-DNA ASOs (unlike RNase H mech) and higher activity than corresponding gapmers! Led by @disha_kashyap_ @UCLChemistry @OxfordChemistry
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New preprint 🎇 We developed oligonucleotide PROTACs (OligoPROTACs), which show comparable activity to small molecule equivalents - but can be *switched off*, using DNA nanotech, to restore protein levels! Last of @disha_kashyap_'s trilogy! @milnetom68
https://t.co/hW8lrV7ghb
DNA-programmable Protein Degradation: Dynamic Control of PROTAC Activity via DNA Hybridization and Strand Displacement https://t.co/VQOkB6gzbR
#chemrxiv_biochem
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🚨 Preprint alert 🚨 We have developed an entirely new mechanism of action for ASO gene knockdown -> lysosomal trafficking 🧬! Works with non-DNA ASOs (unlike RNase H mech) and higher activity than corresponding gapmers! Led by @disha_kashyap_ @UCLChemistry @OxfordChemistry
Engineering antisense oligonucleotides for targeted mRNA degradation through lysosomal trafficking https://t.co/PD4Kv8lKxZ
#chemrxiv_biochem
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Massive congratulations to @DrZoeWaller for winning the 2024 @RoySocChem Chemical Biology and Bioorganic Lectureship - today, giving her fantastic award lecture at the 2025 RSC CBBG Forum, in Bristol 🥳🏆
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Still time to apply for this #PhD project to join my group and @DrMikeBooth group at @UCLChemistry Project is part of #CDT in #Engineering Solutions for #Antimicrobial #Resistance App #Deadline: 26th Jan CDT #OpenDay : 14th Jan on Zoom More info 👉
particlemicrofluidics.com
#Phd project available to join my group and @DrMikeBooth's group at @UCLChemistry on the #CDT in #Engineering Solutions for #Antimicrobial #Resistance to work on electrokinetic and nucleic acid technology for lateral flow assays ( https://t.co/x4DG1lOxJR) App Deadline: 26/01/25
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Read our review on next-generation covalent modifications for nucleic acid therapeutics! 🧬💊
We just published a review in ACS Bio & Med Chem Au on the state-of-the-art and future of covalent nucleic acid conjugates as therapeutics 🧬💊! Led by @disha_kashyap_
@ACSBioMed @ACS_DivBioChem
@UCLChemistry @OxfordChemistry @chem_in_cells
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One of the (hopefully) useful things we did as part of this review is put together a table of all the currently approved short oligo therapeutics, their target/mechanism of action, and their CHEMISTRIES! We didn't manage to find anything out there like this!
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We just published a review in ACS Bio & Med Chem Au on the state-of-the-art and future of covalent nucleic acid conjugates as therapeutics 🧬💊! Led by @disha_kashyap_
@ACSBioMed @ACS_DivBioChem
@UCLChemistry @OxfordChemistry @chem_in_cells
pubs.acs.org
Nucleic acids have emerged as a powerful class of therapeutics. Through simple base pair complementarity, nucleic acids allow the targeting of a variety of pathologically relevant proteins and RNA...
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#ASO nuclear localisation unlocked via (+)-JQ1 conjugation! We’ve enhanced the splice switching activity and RNase H-recruitment of antisense oligonucleotides🧬 One of my main DPhil projects #RNAtherapeutics! @DrMikeBooth @milnetom68
@chem_in_cells @OxfordChemistry @MRC_WIMM
We have dramatically increased the activity of splice switching *and* RNase H-active antisense oligonucleotide #ASO therapeutics by conjugating a nuclear importer! Preprint led by @disha_kashyap_
@milnetom68 @chem_in_cells @OxfordChemistry @UCLChemistry
https://t.co/6j0sW0TbQU
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JQ1 4EVA! @jaybradner @JunQiLab
We have dramatically increased the activity of splice switching *and* RNase H-active antisense oligonucleotide #ASO therapeutics by conjugating a nuclear importer! Preprint led by @disha_kashyap_
@milnetom68 @chem_in_cells @OxfordChemistry @UCLChemistry
https://t.co/6j0sW0TbQU
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Fascinating work showing that the covalent addition of a nuclear importing compound, JQ1, to therapeutic oligonucleotides enhances their activities by enhancing nuclear penetration.
We have dramatically increased the activity of splice switching *and* RNase H-active antisense oligonucleotide #ASO therapeutics by conjugating a nuclear importer! Preprint led by @disha_kashyap_
@milnetom68 @chem_in_cells @OxfordChemistry @UCLChemistry
https://t.co/6j0sW0TbQU
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Super congratulations! 👍👍👍
We have dramatically increased the activity of splice switching *and* RNase H-active antisense oligonucleotide #ASO therapeutics by conjugating a nuclear importer! Preprint led by @disha_kashyap_
@milnetom68 @chem_in_cells @OxfordChemistry @UCLChemistry
https://t.co/6j0sW0TbQU
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Really cool design: sending ASO to nucleus via conjugating JQ1.
We have dramatically increased the activity of splice switching *and* RNase H-active antisense oligonucleotide #ASO therapeutics by conjugating a nuclear importer! Preprint led by @disha_kashyap_
@milnetom68 @chem_in_cells @OxfordChemistry @UCLChemistry
https://t.co/6j0sW0TbQU
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