Chris Reinhardt
@ChrisJReinhardt
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NIH NRSA Postdoc Fellow, Cravatt Lab at @scrippsresearch | Chemistry PhD @ChanLabUIUC at @ChemistryUIUC | Chemistry & Biology BS Richard Lab at @UBuffalo
San Diego, CA
Joined December 2017
Check out @Michael_A_Erb's wonderful tweetorial on our recent paper. It was a pleasure to contribute to this story. Congratulations to all the authors. Modulating 🦊A1 with covalent small molecules.
Together with the Cravatt group, we have a new manuscript out reporting the chemoproteomic discovery of covalent FOXA1 ligands that bind its DNA-binding domain, corrupt its motif preferences, and redistribute it on the genome. 1/11
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Best practices statements are helpful for promoting reliable technologies across different and multidisciplinary disciplines. The probe development field expanded dramatically over the past couple decades and recommended conditions/controls may help increase their use.
CONSENSUS STATEMENT. Reactive oxygen species are complex & difficult to measure. A group led by Murphy & Halliwell (@Cambridge_Uni, @NUSingapore) proposes guidelines on ROS nomenclature & assessment in cells, tissues & in vivo.
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Nice reminder from @INSTMohali that sensors can alter biology. NO sensor (Urea-eTCBD) is anti-inflammatory at low μM concentrations. Additional evidence to support NONOate over N-nitroso formation (e.g., 15N NMR) and whether the adducts can release NO would be interesting.
Efficient Nitric Oxide Scavenging by Urea-Functionalized Push-Pull Chromophore Modulates NO-Mediated Diseases (Ghosh, Jayamurugan, et al.)
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RT @JarrettRenn: Very excited to share this story, now in Press at @MolecularCell! With @michaellazear, @jaegemar11, and contributions from….
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RT @ThomasBPoulsen: ✴️NEW ELECTROPHILES✴️ .We continue a bit of a streak of new reagents for protein/peptide functionalization. Check out t….
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CATCH - an exciting approach for monitoring compound distribution and target engagement in cleared tissue. High resolution imaging reveals tissue, cellular, and subcellular target shifts in vivo. Congratulations to all that contributed! @liye_tsri @SchafrothMA @scrippsresearch.
Excited to share new paper @CellCellPress. Led by Zhengyuan Pang @ScrippsGradPrgm, collaboration w/ Ben Cravatt, CATCH is a chem-bio innovation to allow imaging of drugs in tissue. This "mission impossible" only happens with the incredibly collaborative @scrippsresearch . 1/3.
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The concentration of truncated dye could then be used to report on the local environment of the cells during phototruncation and read out on downstream phenotypic changes. Congratulations @SchnermannLab, @sid_matikonda, @LenkaStackova!.
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I am curious about the O2-dependence of phototruncation, particularly in the context of cancer. If the rate of phototruncation is sensitive to (patho)physiological O2 levels, then the yield may differ between hypoxic/normoxic tissues.
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Interesting strategy to track cells in live animals by leveraging light-induced cyanine phototruncation. This represents a nice example of applying mechanistic understanding ( to develop a tailored dye platform with exciting applications.
pubs.acs.org
The light-promoted conversion of extensively used cyanine dyes to blue-shifted emissive products has been observed in various contexts. However, both the underlying mechanism and the species involved...
Excited to see #Cyanine PACT out on @ChemRxiv today. This straightforward method enables spatiotemporal control over cell tracking. @SchnermannLab @LenkaStackova @FredNatLab @NCIResearchCtr @theNCI #Hisataka_Kobayashi #chemtwitter.
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Interested in detecting nitric oxide (NO) in live cells or animals with bioluminescence imaging? Consider using BL660-NO, an NO-gated wild-type firefly luciferase substrate. BL660-NO was then used to evaluate the effect of diet on NO in a murine cancer model. 🔦🧫🐭🍔@ChanLabUIUC.
Check out our latest article in @ACSCentSci entitled Activity-Based NIR Bioluminescence Probe Enables Discovery of Diet-Induced Modulation of the Tumor Microenvironment via Nitric Oxide. Congrats to Anuj, Michael, Melissa, Su, and @ChrisJReinhardt!! 🥳🥳
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Enabling new disconnection for synthesizing chemiluminescent luminophores. I look forward to seeing what this approach yields in the way of next-generation, designer chemiluminescent platforms.
Presenting a game-changer synthetic route for preparation of adamantyl-enolether as precursors for dioxetane chemiluminescent luminophores. Thanks to @BaranLabReads for a great collaboration. Now appearing in Angewandte Chemie, Accepted Articles:.
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RT @VocadloGroup: Newest publication led by @DustinTKing is out in @J_A_C_S. Using thermal proteome profiling, we studied the effects of O-….
pubs.acs.org
Posttranslational modifications alter the biophysical properties of proteins and thereby influence cellular physiology. One emerging manner by which such modifications regulate protein functions is...
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RT @mads_chem: Congratulations to the 2022 @WolfPrize_ #chemistry laureates Prof. Ben Cravatt @scrippsresearch , Prof @CarolynBertozzi and….
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Today’s unexpected discovery: gel-loading pipet tips make excellent TLC spotters.
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RT @katecarroll_lab: Old chemistry, new tricks @NatureChemistry ! Repurposing Wittig reagents for redox biology. See our collaborative work….
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RT @LindorffLarsen: Comparing enzyme turnover rates in vitro with in vivo activities in E. coli and S. cerevisiae.h….
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RT @fordycelab: Excited to announce our newest paper published today! How do we get to accurately predicting *function* from sequence? It….
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Interesting work from Natasha Vetter and @DavePalmerUSask. The authors use C-phosphonate substrate analogs and phosphite rescue experiments to dissect the role of dianion activation in kanosamine biocatalysis. Brings back wonderful memories from my time in @jprichard53's lab.
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Beautiful example of controlling biology with light from @DirkTrauner's group. I am curious whether this strategy can completely block dark engagement or if protein flexibility allows the enzyme to adopt a competent conformation for Cys reactivity, albeit at slower rates.
Check out our new paper on arming a cysteine-reactive electrophile with light. Thanks to a collaboration with Stefan Laufer and Stefan Knapp. @goetheuni. @uni_tue. @nyuchemistry. @photopharma_lit
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RT @ChanLabUIUC: Check out our latest @J_A_C_S article on the development of a NIR-II photoacoustic probe for nitric oxide! Great work Meli….
pubs.acs.org
Photoacoustic (PA) imaging has emerged as a reliable in vivo technique for diverse biomedical applications ranging from disease screening to analyte sensing. Most contemporary PA imaging agents...
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