Flo Chardon
@FloChardon
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Scientist and computational biology team lead at the Seattle Hub for Synthetic Biology at the Allen Institute. PhD with @JShendure and @lea_starita.
Seattle, WA
Joined February 2014
I spent my PhD developing novel genomic technologies, and today I am extra excited to share the last bit of this work, which is a multiplex prime editing (PE) screening framework! (1/n)
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I have only amazing things to say about @sdomcke - her lab will be one of the best out there, both from a scientific and mentorship perspective. I can't wait to visit!.
New synthetic biology / gene regulation lab opening in Zurich! We’re studying how to control cell state transitions – for example, making diseased cells healthy or creating new cellular functions – using systematic perturbations, single-cell genomics and machine learning. 🧵1/4
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RT @Nobu_Hamazaki: News & Views article by Jesse and Alex on our RA-gastruloid model! Big thanks to @alex_schauer_ and @jvveenvliet for the….
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Exciting times!!!.
Incredibly proud to share that I recently joined @UCSF_BTS @UCSF as an Assistant Professor! This means that the Calderon Lab is now open!. Or, more accurately, in the process of opening as I am still unboxing equipment and unraveling giant posters (a last @JSHendure lab gift) 🥳
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Another phenomenal piece of work from a superstar postdoc and my closest collaborator @TroyMcDiarmid, & incredible (now PhD student!) Megan Taylor! Megan joined the lab as a freshman undergrad, & it has been nothing short of amazing watching her develop into an amazing scientist.
Beyond stoked to share our latest, entitled “Diversified, miniaturized and ancestral parts for mammalian genome engineering and molecular recording” !.
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Thanks @JShendure for the second-to-none mentorship and scientific guidance, and a big thank you to @NadavAhituv for your guidance and prudent insights in regards to the development of this method and work!.
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I also feel lucky that my interests outside of lab aligned so well with so many Shendure lab members! How special is it to have labmates that will go skiing with you before work? :) (also pictured is @sdomcke, another wonderful collaborator)
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Troy is one of a kind. He is off the charts brilliant, he is efficient yet meticulous and detail oriented, he has a new dad joke for me and the lab every day, and he is just a pure joy to be around.
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I often tell people how much I loved grad school, and this project was a big reason why! I also feel like the luckiest scientist to have the absolute best collaborators, who have become my very good friends, especially @TroyMcDiarmid.
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The cell type specificity we see, particularly at enhancers, is my favorite observation from this work. It was a treat to be able to do this screen in iPSC-derived neurons and see the striking contrast in enhancer upregulation btwn K562 cells and neurons!
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This finding is particularly neat, as this was 1 gRNA out of 20 tested, and it is the same exact gRNA that was used in rescue studies in SCN2A+/− haploinsufficient mice and human iPSC-derived neurons - beautiful work from @NeuroBender and @NadavAhituv.
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Not only did SCEPTRE find many of the same hits as our original approach (>80%), SCEPTRE found additional hits, including a gRNA that upregulates SCN2A exclusively in neurons.
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Additionally, we analyzed our data w/ SCEPTRE, a framework developed by @RoederKat & @EugeneKatsevich et al. SCEPTRE is based on conditional resampling & integrates covariates to calibrate the statistical assessment of results in single cell CRISPR screens.
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Namely, we successfully validated our single-cell findings in singleton, bulk RNA-seq experiments and observed the expected upregulation in 11/13 cases.
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A detailed post describing the method is here: but I am excited about the additions we have made to the paper since its first posting on bioRxiv.
Absolutely thrilled to share mine and @TroyMcDiarmid’s work describing the development of a multiplexed, scalable CRISPRa cell type-specific screening framework that is able to identify…(1/n)
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Genomic tech dev is my favorite area of science, and today our paper describing our multiplex CRISPRa screening method to identify cell type specific regulatory elements is published!
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RT @hcmefford: Another opportunity @StJudeResearch! Seeking a senior scientist 👨🔬👩🔬to lead a brain #organoid core to support researchers….
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RT @Nobu_Hamazaki: Excited to share our human RA-gastruloid model in @NatureCellBio! Early retinoic acid supplementation, followed by later….
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So, so proud of @wchenomics. I have all the respect in the world for Will and his devotion to seeing this through to the end! This represents an absolutely enormous amount of experiments and high quality science. Congrats!.
Super excited to share that ENGRAM is out today! ENGRAM cells are programmed to write their histories into the genome, recording the intensity, duration, and order of biological events simultaneously.
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RT @NIChardon: What do microhabitat suitabilities above range edges tell us about potential species distribution shifts?! Huge thank you to….
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RT @hcmefford: We're so excited to continue our collaborative work with @CarvillLab and @curechd2 on the CHD2 episignature. Thx to @ebonkow….
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