Gerald Schwank
@schwanklab
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Associate Professor @UZH_en. We develop genome editing approaches for personalised treatment of rare diseases and cancer.
Zurich, Switzerland
Joined December 2017
Congrats to the entire team and collaborators @uzh @AcuitasTx @KispiZuerich @ETH @upenn. Step-by-step we are getting closer to clinical translation of prime editing to treat metabolic liver diseases!.
Our paper describing an RNA-LNP prime editing strategy for treating phenylketonuria was published today in @natBME (1/7).@schwanklab @UZH_Science @UZH_en @KispiZuerich @ETH_en.
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RT @MartinPacesa: Have you ever wanted to design protein binders with ease? Today we present 𝑩𝒊𝒏𝒅𝑪𝒓𝒂𝒇𝒕, a user-friendly and open-source pip….
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Many thanks to @UZH_en @UZH_Science @UZH_IPT @ETH_en and @nicopmat @Amina_mollaysa #SaphiraMueller @KisslingLucas @tanjarothgangl @LSchmidheini @p_kulcsar #AhmedAllam and collaborators from the #ManfredKopf and #ToniCathomen labs!.
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6) To apply TnpBmax in vivo we delivered it together with a PCSK9-targeting ωRNA via AAV9 into mice. This led to a robust PCSK9 knockout in the liver and a reduction in LDL-cholesterol levels
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5) ISDra2 TnpB requires a 5'-TTGAT TAM site to bind and cleave DNA. While this makes TnpB highly specific it also restricts its target range. Using structure-guided protein engineering we mutated amino acid 76 to altered the ISDra2 TnpB TAM preference from TTGAT to TTTAT or TTCAT
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4) Using this dataset, we developed TEEP, a machine-learning model to predict ωRNA efficiencies and help select potent ωRNAs. The model is freely available via
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3) We noticed that the activity of the TnpB system is strongly influenced by the ωRNA sequence that targets the DNA. This led us to screen over 10,000 target sites in cultured human cells to measure cleavage efficiency in high throughput.
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2) To enhance the editing efficiency of ISDra2 TnpB in mammalian cells, we optimized its DNA sequence to match human codon usage and rearranged NLS sequences for nuclear uptake. Our design resulted in a TnpBmax system with a 4.4-fold improvement in editing efficiency.
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1) TnpB is an RNA-guided endonuclease encoded on the IS200/IS605 transposon element and evolutionarily related to CRISPR-Cas12 proteins. A recent study from the Siksnys lab showed that ISDra2 TnpB can be programmed to induce targeted DNA breaks in human cells
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Excited to share our latest study!.Today in @naturemethods the @schwanklab and @krauthammerlab report in @marquark et al. 'Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs' (1/6).
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RT @sidichen: Our first in vivo primary NK screen that discovered new NK cellular checkpoints. Kudos for team for their 5 years of work. ht….
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RT @nicopmat: Our paper exploring the effect of chromatin on prime editing and diverse pegRNA designs was published today! 👨💻🧬🎉. https://t.….
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If you want to know the outcome of your prime editing experiment before you even conducted the experiment, visit Congrats to Nicolas and many thanks to all collaborators @krauthammerlab @bvansteensellab.
Machine learning prediction of prime editing efficiency across diverse chromatin contexts
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Join us at the Latsis Symposium on Genome and Transcriptome Engineering organized by @MartinJinek @randall_platt @jcornlab and @schwanklab, with lots of great speakers from academia and industry! 🧬 #Zurich #Switzerland June 13-14. Register at:
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RT @krauthammerlab: 🌟New Postdoc opportunity🌟 for developing machine learning methods in biomedicine and healthcare research is open in our….
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RT @jcornlab: Join me at the Latsis Symposium on Genome Engineering #Zurich #switzerland June 13-14! Register now at .
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RT @marquark: I'm excited to talk @ the #AMLDEPFL2024 conference today together w/ @nicopmat. Join us and @Amina_mollaysa @_bunnech @befco….
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RT @jcornlab: Coming soon! Latsis Symposium on Genome Engineering #Zurich #switzerland June 13-14. Great lineup @Tr….
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