RT @ElowitzLab: Just posted a preprint on our new spatial genomic recording system, baseMEMOIR. Motivation: Cells d….

RT @CellRaiser_: Our first paper is out in @CellCellPress! Led by @born2raisecell, we present a programmable reaction-diffusion system that….

RT @depthsofwiki: The "Ship of Theseus" article has been edited 1792 times since it was created in July of 2003. At present, 0% of the phra….

We're depositing parts on @Addgene and hope that our tools join the MoClo-YTK ecosystem to make yeast synbio more accessible, alongside recent work from @willmshaw, @MoKhalilLab, and @ProfTomEllis (, whose previous tools have enabled a lot of our work.

Other work has also created the AID2 system, which uses 5-Ph-IAA to induce the TIR1-AID complex. Combining these with the previous TIR1 mutations, allow us to build a 5-Ph-IAA-inducible system, which activates at 60X, and doesn't respond to NAA at all!

Recently engineered variants of the TIR1 and AID proteins, which are generally used as degron systems, block them from E3 ubiquitin ligase pathways, and allow for auxins to induce heterodimerization. Our system adapted for yeast uses NAA and achieves 80X fold activation!

The danoprevir-induced system was adapted from the NS3 system first engineered in mammalian cells. DNV induces heterodimerization of a DNA binding and activation domain. It took us some work, but we were able to build a system with low basal and high maximal expression!

We ported an abscisic acid-inducible system from mammalian cells and optimized the activation domains to achieve high maximum expression, and over 300X induced fold change!

Our set of doxycycline-inducible systems were developed after testing three advanced Tet-On variants, has basal expression comparable to background cells, and maximum expression 1.5X higher than the yeast pTDH3 promoter.

Need a small molecule inducible system in yeast? We ported FIVE (doxycycline, ABA, DNV, NAA, and 5-Ph-IAA) from mammalian systems and optimized them!. My work with @rickyolaughlin, @AndrewLezia, @wngamkan97, and @PhilEmmanuele is out in @ACSSynBio today!.

RT @ProfTomEllis: It's finally out. Will Shaw's awesome YTK add-on toolkit for rapid parallel yeast genome engineering is published today….

RT @MrPeanut: . *     *    .🥜   *    . *  ☄️. * . 🌍  *     . *   💫  *. *       time to wish  *     . *. *     Mr. Peanut a….

RT @BintuLacra: Our latest paper is out today (with @axmukund @JoshTycko and many other talented trainees): High-throughput functional char….

RT @wesbos: It's been a while since I've seen something as neat at . It's serverless functions meets myspace? You h….

RT @ElowitzLab: Two decades ago @AvigdorEldar & @BarkaiLab introduced concept of shuttling, in which protein circuits push morphogens away….

RT @sciam: What is … ?. @Jeopardy

RT @newscientist: Scientists want to give robots hands made from living woodlice

RT @ElowitzLab: “Messy” many-to-many protein networks control signaling, transcription, adhesion, etc. Is this a bug or a feature? In this….

RT @WallaceUcsf: Where does the structure of mitochondrial networks come from? In a new paper from Greyson Lewis, we review mitochondrial t….

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