Addgene
@Addgene
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Sharing speeds science! Addgene helps scientists around the world find and share research materials - plasmids, viral preps, antibodies, data, and more.
Watertown, MA
Joined October 2009
Addgene is no longer monitoring or using our X, formerly Twitter, account. Instead, find @addgene on Instagram, TikTok, Facebook, LinkedIn, and emerging microblogging platforms. You can read more about this decision here: https://t.co/DyR9xY2ZS6
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🎊 Happy birthday, @Addgene! 🥳 Today we cell-ebrate you and the 137,289 plasmids you have sent on behalf of 5,822 labs from around the world!🤯 Get to know Addgene's #OpenScience mission: https://t.co/svB6dI7sO6
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Our Cell Line of the Week is SERCA2 - illuminating the sarcoplasmic/ endoplasmic reticulum! 💪 Browse our openly available hiPSC lines & plasmids – fluorescently-tagged to illuminate structures: https://t.co/QCetZaTpQt 📦 Distributed by @Coriell_Science & @Addgene
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The key reagents from our recent CombiCell manuscript are now available on @Addgene
https://t.co/wDYZUdAP6q
@embojournal
https://t.co/dtos8OF4AY
embopress.org
imageimageThe inherent difficulty in manipulating cell surface proteins limits our ability to study cell–cell recognition. This work develops a platform for the combinatorial display of cell surface...
Our new work! Using CombiCells, a platform enabling titration and combinatorial display of cell surface ligands, to investigate the sensitivity and costimulatory requirements of TCRs and CARs https://t.co/JYZyRGLWxP
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Our #CellLineOfTheWeek is ras-related protein - targeting endosomes! 🧫 Our hiPSC lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: https://t.co/QCetZaSS0V 📦 Distributed by @Coriell_Science & @Addgene
#OpenScience
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14. Get your SOXmas presents @Addgene
https://t.co/OTEpEaP43z
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Most of our pHYX plasmids recently published https://t.co/dwghUETf8p are now available @Addgene ( https://t.co/Ley8a9o1r4). And merry Christmas to all! 🎅🎄 @ECCP_lab
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We've previously reported PAM variable PEmax for correcting KRAS mutations, and now our constructs - PEmax-SpG, PEmax-SpG-P2A-hMLH1dn - are available on @Addgene! Check them out! #CRISPR #PrimeEditing
https://t.co/YAp6jAElQE
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Troponin I (slow skeletal) is our #CellLineOfTheWeek! ❤️🔬 Our human iPS cell lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: https://t.co/QCetZaSS0V 📦 Distributed by @Coriell_Science & @Addgene. #OpenScience
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Perspective (OA): "The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene" https://t.co/P87nOWt2vG >15,000 plasmids from >1,000 laboratories ∼40 countries 300,000 shipments to ∼5,000 organizations in ∼100 countries @Addgene @CRISPRchef
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Haemophilus influenzae was the first bacteria to be sequenced but few genetic tools exist for this organism. It has small genome of only 1.8 Mbp and can be easily grown in lab. To speed up synbio research for this organism we created plasmids that now you can get @Addgene
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We're depositing parts on @Addgene and hope that our tools join the MoClo-YTK ecosystem to make yeast synbio more accessible, alongside recent work from @willmshaw, @MoKhalilLab, and @ProfTomEllis ( https://t.co/OaOrAPmc3g), whose previous tools have enabled a lot of our work.
pubs.acs.org
Synthetic biology toolkits are one of the core foundations on which the field has been built, facilitating and accelerating efforts to reprogram cells and organisms for diverse biotechnological...
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You can find us on: Instagram: https://t.co/aC4Op2OYrt TikTok: https://t.co/QWcSJEtpQ9 Facebook: https://t.co/EYbxrSYJiW LinkedIn: https://t.co/VAX7b2nevs Addgene Blog: https://t.co/YWKgf3vTXz
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Click editor plasmids now available on @Addgene
https://t.co/4F0TYO4JpL 🧬🖱️ Looking forward to feedback from the community on how well click editing works in your hands! @FerreiraSilvaJ @connorjtou @EmilyM_King @Madeline_Eller_ @Linyuan79834832 @DavidRufinoRam1
We’re excited to share our work developing a new gene editing approach that we’re calling #clickediting 🖱️✴️🧬. Click editors (CEs) utilize DNA-dependent polymerases to write bespoke edits into the genome. https://t.co/E6DOZD1CWp
@CGM_MGH @MassGeneralNews @MGH_RI @harvardmed
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The plasmids are available on @Addgene, including recombinases, reporters, and empty vectors for your own favorite gene:
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Interested in Transcription Factor high-content screening? we have a dFLASHy new platform for you. dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Plasmids @Addgene
biorxiv.org
Live-cell reporting of regulated transcription factor (TF) activity has a wide variety of applications in synthetic biology, drug discovery, and functional genomics. As a result, there is high value...
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Our #CellLineOfTheWeek is sialyltransferase 1 - targeting the golgi! 🧫 Our hiPSC lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: https://t.co/QCetZaTpQt 📦 Distributed by @Coriell_Science & @Addgene
#OpenScience
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Update: most of the Contact-FP probes are now available on @Addgene. We hope folks will try them out and let us know what you think!
Preprint alert! We are happy to introduce Contact-FP, a suite of probes for studying #MembraneContactSite dynamics. This was an amazing team effort led by Research Associate @GregoryMiner18 and postbacc Sidney Smith 1/11
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🌈plasmids now available at @addgene 🍭 https://t.co/HIWFn1Gcru
Very happy to share a new approach we developed at @institut_curie/@CNRS with my colleague and friend Arnaud Gautier from @Sorbonne_Univ_/@ENS_ULM CATCHFIRE allows to induce and sense protein interaction using a reversible fluorogenic system. More here :
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