Nicholas Perry
@ntperry13
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Scientist, Hsu Lab UCB-UCSF Bioengineering PhD Arc Institute Genome design and bridge recombinases
Palo Alto, CA
Joined August 2018
"Does it work in human cells?" is a question I've gotten a lot this past year. Excited to share our latest work on bridge recombinases! It's been a lot of fun to lead this team effort. If you are excited to hear more, join for my thesis defense tomorrow at 3pm PT. Links below:.
Genomes encode biological complexity, which is determined by combinations of DNA mutations across millions of bases. In new @arcinstitute work, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement.
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Happy to share that I'm continuing my research on bridge recombinases as a scientist in @pdhsu lab at the @arcinstitute! I am looking for a motivated post-grad to work with me as a research associate. If you are interested or know of someone, please use the link below:.
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RT @JuliaBauman2: Fantastic news for genome engineers: Bridge recombinases work in human cells!!🧬. And they can make really big insertions,….
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RT @NikoMcCarty: In the ocean, a single-celled organism travels ~50 meters vertically by rapidly inflating its body with water. The dinofl….
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RT @NikoMcCarty: The Case for Bridge Editors. Many people seem confused about the gene-editing tool released by @arcinstitute. How does it….
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RT @NikoMcCarty: Big deal: Bridge recombinases are now working in human cells. Preprint here:
biorxiv.org
Bridge recombinases are a class of naturally occurring RNA-guided DNA recombinases. We previously demonstrated they can programmably insert, excise, and invert DNA in vitro and in bacteria. Here, we...
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RT @zhangf: We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGR….
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RT @Gabogonzalez515: me with deepresearch:.“find obscure biochemistry papers from the 1960s and 70s that have very weird but intriguing res….
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Congratulations Alison! Looking forward to seeing what people do with your engineered integrases in stem cells and T cells. Check out @AlisonFanton exciting thesis work here:.
nature.com
Nature Biotechnology - Screening recombinases identifies tools for inserting large sequences into the human genome.
Super proud of the fearless @AlisonFanton for her PhD defense yesterday! Alison is a super talented bioengineer and molecular biologist who has done pioneering work on DNA recombinases for human genome insertion. Congratulations, Dr. Fanton!
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RT @mgdurrant: Hey folks, I know most deadlines are fast approaching, but I am applying for assistant professor positions this cycle, and I….
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RT @yaireinhorn: A recent & exciting scientific discovery is Bridge RNA - a new class of guide RNAs that could recombine, insert, delete, o….
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RT @shsternberg: Did you see the bridge RNA papers yesterday? Bacterial transposons promote programmable, RNA-guided DNA recombination. Re….
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RT @shsternberg: On the topic of RNA-guided biology emerging from transposons, you won't want to miss this one! In published work led by th….
nature.com
Nature - RNA-guided transcription factors arose repeatedly via the domestication of transposon-encoded tnpB genes, representing a parallel evolutionary path to CRISPR-Cas adaptive immunity.
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RT @connorjtou: Congrats to @mgdurrant @ntperry13 @pdhsu @hnisimasu and colleagues on their bridge recombinase works! Excited to see how th….
nature.com
Nature - Recombinase enzymes that recognize DNA sequences using a ‘bridge’ RNA.
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RT @patrickc: This morning, Nature published two papers on bridge editing, the new genome engineering technology from @ArcInstitute: https:….
nature.com
Nature - Using cryo-electron microscopy, the structural mechanism by which non-coding bridge RNA confers target and donor DNA specificity to IS110 recombinases for programmable DNA recombination is...
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Finally, plasmids will be @Addgene soon:.Please reach out with any questions on applying bridge recombination to anything you are interested in!.
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Another remarkable mechanism of this enzyme, we think it may have evolved to control directionality of the recombination reaction. We have built the handshake guide reprogramming rules into our bridge RNA design tool, which you can find here:.
arcinstitute.org
Arc Institute is a independent nonprofit research organization headquartered in Palo Alto, California.
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Work from @mgdurrant shows handshake guides appear to be a conserved feature of bridge RNAs, while work from @jjwp1011 shows the identity of the handshake guides have a major impact on recombination efficiency in E. coli.
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