Alison Fanton
@AlisonFanton
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Scientist in the Hsu Lab, Arc Institute
Joined March 2021
especially @BartieLiam and @pdhsu , for your tireless teamwork and support. It’s been a fun PhD!👩🏻🔬
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🧬 Thrilled to share our latest work on engineering precise DNA recombinases for targeted genome integration! Through directed evolution & protein fusions, we achieved over 50% efficiency & 97% specificity for multi-kb DNA cargo insertions. Big thanks to all co-authors -
🧵In new work, we report a systematic engineering roadmap to optimize large serine recombinases (LSRs) for direct, site-specific insertion into the human genome 🧬. We achieved over 50% insertion efficiency and 97% genome-wide specificity, a 10X improvement over our previous work
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@cyrus_tau and I are so excited to share our work on developing a CRISPR protein-guided, trans-splicing system for making versatile RNA edits!!! See the highlights in Cyrus' thread and the preprint for details!
.@sitaritapita and I are excited to share RESPLICE, a new RNA-programmable mode of editing that leverages RNA targeting CRISPR proteins and the endogenous spliceosomal machinery to enable versatile RNA edits in human cells! (1/12) https://t.co/7VjKtA65ub
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Amazing week @KeystoneSymp ! So much fun to finally share our work. It’s been an awesome scientific journey characterizing this system with @mgdurrant and our team Some notes on our bridge recombination system from our preprint 🧵:
Just shared at @KeystoneSymp a new @ArcInstitute discovery of the bridge RNA recombinase mechanism: a new class of natural RNA-guided systems that retains the key property of programmability from RNAi and CRISPR while enabling large-scale genome design beyond RNA and DNA cuts
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By the way, if this flavor of molecular technology development excites you, we are hiring (at all levels)! Please reach out 👩💻🧑💻👩🔬👨🏻🔬
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The ability of molecular biologists to control DNA has relied on enzymes derived from the phage-microbe arms race. In this new work, we systematically mined thousands of new serine integrases (LSRs) and developed them for human genome editing. 🧬 https://t.co/tjJKBvRPYc
biorxiv.org
Recent microbial genome sequencing efforts have revealed a vast reservoir of mobile genetic elements containing integrases that could be useful genome engineering tools. Large serine recombinases...
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