At 15, I left all my family and friends and came to the US , ALONE, seeking a country where MERIT and HARD WORK mattered-not politics. For 30 years, I worked tirelessly, even doing reseach at MIT the day my mother died, knowing she’d want me to keep pushing forward. Today, my

Also happy to talk about my experience the past two years here. TLDR: it’s been wonderful!

The Regev lab at Genentech is recruiting for a 1-year research associate position (summer), ideal for a recent BS/MS who is taking a gap year prior to graduate school. Particular experience in molecular biology techniques are very appreciated. Please RT/send around!!!

🧬GWAS is fundamental in drug discovery, linking disease to genetic variants. However, studying rare and uncommon diseases with GWAS is hard due to the huge sample sizes required. How can we use AI to help GWAS with small cohorts? In a multi-year collaboration @GSK

Getting increasingly excited about recent tech like this that will enable us to simultaneously perturb multiple genomic loci! One step closer to the “virtual cell” as we explore the combinatorial phenotypic space.

Thrilled to share our new manuscript combining Perturb-seq (pooled CRISPR screens + scRNA-seq readouts) with Cas12a and its unique guide RNA processing ability. Led by the extremely talented Valentina Snetkova at Genentech :)!

Gene regulation involves thousands of proteins that bind DNA, yet comprehensively mapping these is challenging. Our paper in @NatureGenet describes ChIP-DIP, a method for genome-wide mapping of hundreds of DNA-protein interactions in a single experiment. https://t.co/0aINMj2MTu

Excited to share Sarah's new review on BiDirectional Promoters (BDPs). Sarah discusses 6 molecular mechanisms that may account for unique transcriptional properties of BDP with a focus on noise regulation and co-expression: https://t.co/p0zNDvfTKw

Does anyone know of any organizations or non-profits or other outlets (this can be interpreted very broadly)? If so, DM me or reply please! :) (2/2)

Hey fellow Bay Area science twitter: I’m a current research tech who just graduated from undergrad last year. I want to get more involved in (1) DEI efforts for underrepresented students in STEM/research and (2) science outreach/communication efforts. (1/2)

An elegant solution for 2-plex perturbation screens! And great care was put into minimizing lentiviral recombination. Great work 🤓

(1/2) New paper from KL Kim @GilbertRahme @BradEBernstein @GRO_Broad with RCMC contribution from @ViraatGoel @hansen_lab https://t.co/aQh8jDIacp Quantitative dissection of how loss of a CTCF insulator boundary in GIST causes aberrant enhancer-oncogene interactions

How we think and talk about mitochondria matters to our science and to newcomers in the field. The powerhouse analogy is expired and we need specific vocabulary to capture the beautiful complexity of mitochondrial biology. We propose a framework and some nomenclature.

So much to learn still about enhancer-promoter interactions! 🥸🤓

🗣️wahoo!!! my doctoral work is out online in @CellCellPress: https://t.co/OBbpudQRvq!! couldn't have done it without my two wonderful labs and co-advisors @stanleyqilab & @MackallLab we developed a new RNA-targeting CRISPR platform (MEGA) to engineer better T cell therapies...🧵

Our latest in @Nature: Convergence of coronary artery disease genes on endothelial cell programs https://t.co/5B8tB3IeXI Incredible work led by Gavin Schnitzler and @kanghelenyihua — a long-standing partnership between our lab and @Dr_RajatGupta My comments below: 1/

SC-VAE: A Supervised Contrastive Framework for Learning Disentangled Representations of Cell Perturbation Data https://t.co/gx4SJCGRfk

PerturbView enables in vivo CRISPR screens with single cell spatial resolution ( https://t.co/DLQBJh7u76). CRISPR sgRNA are uniquely identified over 6 rounds of in situ sequencing. Attached is an animation of in situ sequencing of sgRNAs in a tumor xenograft (1/4)

Spatial analysis is converging with pooled CRISPR screens in a recent series of preprints w/ different guide readouts: 1. RCA https://t.co/aLqqrkfPuz 2. in situ txn + FISH https://t.co/tmhSGOA82u 3. in situ txn + in situ seq https://t.co/ICotfgI1dj Exciting for tissue biology!

Check out the new optical pooled screening (OPS) technology, PerturbView, from my fantastic mentor, Taka Kudo! ⬇️ PerturbView enables image-based perturbation screens in tissue with spatial transcriptomics phenotyping.