RT @nkalogriopoulos: I’m beyond excited to share that I’ll be starting my own lab at the University of Wisconsin–Madison School of Pharmacy….

We are hiring a new lab manager! Our wonderful lab manager Albert is headed off to grad school and we are looking to fill his position this summer. This is a great opportunity for a new graduate to spend a couple years immersed in research before applying to PhD or MD programs.

🚨 Funded postdoc opportunities in the U.S. and Japan—spend time abroad! 🚨. The Chan Zuckerberg Biohub Network and the Stellar Science Foundation are inviting postdoctoral fellows to apply for the new Global Science Scholars fellowship!. This 2-yr fellowship supports your next.

RT @ldtimmerman: NIH cuts are hitting young scientists the hardest. By David Baker.

Today we report the directed evolution of HUH (a sequence-sequence covalent protein tag for ssDNA labeling) into rHUH, a sequence-specific covalent protein tag for RNA labeling. rHUH was hard to engineer: the initial HUH template had no RNA activity, so we started with RNA-DNA

Tetanus on demand! (not for bioweapons, but for neuroscience research). Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release. To harness this toxicity and use it as a beneficial tool for

PAGER plasmids are now available on Addgene:

RT @AndrewHires: We need private water tankers to come to Palisades Highlands NOW. @LADWP has sent water trucks but they are sitting unused….

Are you a Japanese student interested in a postdoctoral fellowship in the US? The Chan Zuckerberg Initiative has an exciting new fully-funded Global Science Scholars program to pair students who have received their undergraduate or doctoral degrees in Japan with US bioengineering.

RT @mfgrp: Today we report that an engineered skin bacterium, swabbed gently on the head of a mouse, can unleash a potent antibody response….

Hear from PAGER’s co-creators, @nkalogriopoulos and @ReikaTei in this Stanford news story from @Taylor_Kubota:.Tool that enhances control of cellular activity could expand biological and medical frontiers.

This work was co-led by two extraordinary postdocs, @nkalogriopoulos and @ReikaTei. We also had the privilege of collaborating with @yulonglilab and Yuqi Yan on PAGER-FL, and @SolteszLab and Peter Klein on the application of PAGER-Gi in brain slice. In our lab, @MatthewRavalin.

To demonstrate the utility of PAGER, we used it for antigen-control of neuronal excitation and inhibition, in both culture and acute brain slices. We also used PAGER in the cancer immunology context. PAGER-TF sensed tumor antigens and secreted an anti-CD3/anti-CD19 BiTE.

We showed that PAGER is modular by inserting 15 different GFP or mCherry-binding nanobodies, as well as 13 nanobodies against growth factors, chemokines, cell surface receptors, etc., to create new antigen-activated synthetic receptors. No optimization, linker screening, or

Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from transgene expression and real-time

RT @tinakim_neuro: Happy to share the lab’s latest research tagging psychedelic-activated neurons in mice. See the post below from first au….

This work was led by co-first authors Song-Yi Lee and @heegwangroh, and we are extremely grateful to our collaborators in the labs of Miguel Alcalde, Mark Ellisman, @RogelioHdezL, Steve Carr, @namude, and Liqun Luo. This study would not have been possible without the amazing.

In the process of engineering LaccID, we found that it was inactive in the cell interior but active at the cell surface, because the 4 copper ions required for its activity are inserted in the Golgi or post-Golgi compartment. We turned this liability into an advantage, and found.