Alex González-Delgado
@alex_gonzadel
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Postdoctoral fellow at Gladstone Institutes
San Francisco, CA
Joined June 2011
Glad to share our review on Prokaryotic Reverse Transcriptases (RTs) now published in @FEMSmicro Reviews! If you are interested in CRISPR, Retrons, Abi, or novel defense systems which includes RTs check this out👇 (THREAD) https://t.co/y89E5eWS12
academic.oup.com
Prokaryotic reverse transcriptases (RTs) are attracting considerable attention, both for use in cutting-edge technologies, such as genome editing, and as a
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Reverse transcribed ssDNA derepresses translation of a retron antiviral protein https://t.co/N0h3JwTXtQ
#biorxiv_micrbio
biorxiv.org
Retrons are bacterial immune systems that prevent the spread of phages by initiating a toxic response within infected hosts. All previously characterized retrons produce high levels of multicopy...
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Kudos to @thekarenzhang @matromont @Darshini_Poola for leading & thanks @seth_shipman @alex_gonzadel for bringing us in with @g1_guillermo @ArturoCarabias! Super proud of @pentamorfico, @Dennis_J_Zhang Josepha Klas, & Ruiliang Zhao for all the fantastic work from our side! 👏🧬
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A mysterious retron with a not-so-non-coding RNA!🧬 Super fun collab with the Shipman lab🚀Learned a lot from their creative setups (check them out!!)💡
Been working on a really strange retron bacterial immune system, here's the preprint: https://t.co/iWP8qH1Fe7 Type VI retrons are unlike any other. Phage infection triggers reverse transcription of a DNA fragment that activates translation of a toxin to kill the infected cell.
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Large collaborative effort here: @thekarenzhang, @matromont, @Darshini_Poola, Josepha Klas, @ArturoCarabias, @Dennis_J_Zhang, @pentamorfico, Ruiliang Zhao, Ana Dávila Hidalgo, @g1_guillermo, @rafapinilla92, @alex_gonzadel
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Been working on a really strange retron bacterial immune system, here's the preprint: https://t.co/iWP8qH1Fe7 Type VI retrons are unlike any other. Phage infection triggers reverse transcription of a DNA fragment that activates translation of a toxin to kill the infected cell.
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Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. https://t.co/OlU6HOLQfK
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Check out MitoScribe, our new preprint led by @Linhan_W: https://t.co/rvNOSaMily... It's an analog molecular recorder that uses neutral base edits to the mitochondrial genome to store info about historical signaling in a cell. Single cell resolution at scale (see next post)!
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New Preprint!! @alex_gonzadel accomplished a major feat on this one: ported retron recombineering, which we love so much in E. coli, into 14 new bacterial species via a massive collaborative effort involving 9 labs! https://t.co/EtUEe3ZYvk
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The June issue is live https://t.co/9NpFgB6SMV On the cover, Khan et al. present a molecular census of retrons, performing the same battery of tests across many distinct systems to identify similarities and differences https://t.co/1fo89H2G86
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C. acnes is the most abundant bacterium on human skin. But what if we could make it the smarterst? 🦠✨ In our new @cellsystems study, we programmed C. acnes to actively protect the skin for therapeutic applications! 🛡️ https://t.co/hb9JjqXyMs
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🧬🧬 𝙉𝙚𝙬 𝙋𝙧𝙚𝙥𝙧𝙞𝙣𝙩!!🧬🧬 Latest collab with the amazing Ming Li & Yue Feng labs! How do phages bypass multi-layered defenses in bacteria? Spoiler: with equally complex anti-defenses!! 🧵👇
biorxiv.org
In prokaryotes, arrayed CRISPR RNAs (crRNAs) guide Cas proteins to destroy phage DNA/RNA, while solitary crRNA-like RNAs (crlRNAs) program Cas proteins for auto-regulation or to control abortive...
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Excited to share our last collab paper in @NatureComms on the structure & interference mechanism of the mysterious 𝗧𝘆𝗽𝗲 𝗜𝗩-𝗔 𝗖𝗥𝗜𝗦𝗣𝗥-𝗖𝗮𝘀 system!! 🧬🚀 🔗 https://t.co/kPzhs73Ffi 🧵 by @patrick_pausch below!
nature.com
Nature Communications - Type IV-A CRISPR-Cas systems diverge from the general CRISPR-Cas mechanism. To understand this system, the authors determine cryo-EM structures of two evolutionarily...
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The role of noncoding RNAs in bacterial immunity Review by Rafael Pinilla-Redondo and colleagues @pentamorfico @lihuijuan20 @rafapinilla92
https://t.co/HPNTonN7SR
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Gladstone scientists undertake a major effort to examine the diversity of retrons, bacterial defense systems that can be leveraged for precise gene editing. @seth_shipman
@Asim_g_Khan
@matromont
@alex_gonzadel
@NatureBiotech
@UCSF
@UCSF_BTS
@czbiohub
https://t.co/ob79O8HjRT
gladstone.org
Gladstone scientists undertake a major effort to examine the diversity of retrons, bacterial defense systems that can be leveraged for precise gene editing
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An experimental census of retrons for DNA production and genome editing https://t.co/oHEEfQZT99
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An experimental census of retrons, now published in @NatureBiotech. We synthesized more than a hundred new retrons and put them through their paces, improved recombineering rates and precise editing in human cells up to 40%.
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The link for the faculty position is now posted! Deadline is October 15th. Please share widely with talented scientists in your network. Come join us at UCSF! https://t.co/Gccrtpl8ym
Search: Tenure track Faculty Position @UCSF in the Dept. of Micro and Immuno, with lab space at the Mission Bay campus! We seek a creative investigator with a strong independent research plan who will be a great mentor, colleague, and peer. Job ad soon. Assistant Prof level.
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A team at Gladstone developed a technology that lets them edit the genomes of bacteria-fighting viruses, known as phages, in a streamlined and highly effective way. @seth_shipman
@katecrawdaddy
@ChloeFishman
@NatureBiotech
@UCSF
@UCSF_BTS
@czbiohub
https://t.co/Dkfk5cGMGT
gladstone.org
Scientists at Gladstone Institutes develop a streamlined and rapid way to engineer bacteria-fighting viruses known as phages
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Multiplexed editing of phage genomes using retrons, now published at @NatureBiotech with lots of new bells and whistles. @ChloeFishman @katecrawdaddy @BhattaraiKline @Darshini_Poola @thekarenzhang @alex_gonzadel @matromont
https://t.co/rkVtTEhVo1
nature.com
Nature Biotechnology - Phage genomes are readily engineered using retron recombineering.
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