Darshini Poola
@Darshini_Poola
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incoming doctoral student @UTAustin | Previously: RA @GladstoneInst | BS-MS @IISERPune 2019-24 | Bioengineering | Painting, Crocheting, Digital Art | Bookworm
Joined June 2020
Now published @NAR_Open! SspA is a transcriptional regulator of CRISPR adaptation in E. coli.
academic.oup.com
Abstract. The CRISPR integrases Cas1-Cas2 create immunological memories of viral infection by storing phage-derived DNA in CRISPR arrays, a process known a
New preprint from the lab, on the fundamental biology of CRISPR adaptation! Check out the preprint here: https://t.co/4JTpM4C5An Or, start with this wonderful highlight thread by @oso_de_antojos who led the work. @GladstoneInst @UCSF @UCSF_BTS @BioE_Beast
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🚨New Pub: High throughput variant libraries and machine learning yield design rules for retron gene editors, now out in @NAR_Open. This is a great one, and the last bit of @katecrawdaddy's PhD, who is off to David Bakar's Lab for a postdoc. https://t.co/MVEWd7jVJ2
academic.oup.com
Abstract. The bacterial retron reverse transcriptase system has served as an intracellular factory for single-stranded DNA in many biotechnological applica
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ICYMI: "An experimental census of retrons for DNA production and genome editing" in @NatureBiotech
https://t.co/QuYm4EkygI
nature.com
Nature Biotechnology - Genome editing tools are developed through large-scale characterization of bacterial retrons.
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Have you seen this new publication from the Shipman Lab? Shoutout to the team and collaborators ⤵️ ⤵️ @seth_shipman
@Asim_g_Khan
@matromont
@alex_gonzadel
@NatureBiotech
@UCSF
@UCSF_BTS
@czbiohub
https://t.co/QuYm4EkygI
nature.com
Nature Biotechnology - Genome editing tools are developed through large-scale characterization of bacterial retrons.
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Again from @seth_shipman lab @GladstoneInst, a compendium of retrons!
nature.com
Nature Biotechnology - Genome editing tools are developed through large-scale characterization of bacterial retrons.
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🚨 NEW PUBLICATION 🚨 "An experimental census of retrons for DNA production and genome editing" @seth_shipman
@Asim_g_Khan
@matromont
@alex_gonzadel
@NatureBiotech
@UCSF
@UCSF_BTS
@czbiohub
https://t.co/QuYm4EkygI
nature.com
Nature Biotechnology - Genome editing tools are developed through large-scale characterization of bacterial retrons.
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An experimental census of retrons for DNA production and genome editing https://t.co/oHEEfQZT99
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An experimental census of retrons, now published in @NatureBiotech. We synthesized more than a hundred new retrons and put them through their paces, improved recombineering rates and precise editing in human cells up to 40%.
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New technology could lead to alternative treatments for antibiotic-resistant bacteria #NBTintheNews via @GladstoneInst
gladstone.org
Scientists at Gladstone Institutes develop a streamlined and rapid way to engineer bacteria-fighting viruses known as phages
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@GladstoneInst Phage therapy could leverage retrons to overcome antibiotic resistance #NBTintheNews via @GENbio
genengnews.com
Antibiotic resistance could be overcome if phage therapies were produced more efficiently, for example, through the use of recombitrons.
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A team at Gladstone developed a technology that lets them edit the genomes of bacteria-fighting viruses, known as phages, in a streamlined and highly effective way. Read more about this new study: https://t.co/Dkfk5cGMGT
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🚨 NEW PUBLICATION 🚨 "Continuous multiplexed phage genome editing using recombitrons" @seth_shipman
@katecrawdaddy
@ChloeFishman
@NatureBiotech
@UCSF
@UCSF_BTS
@czbiohub
https://t.co/endf13Gqrw
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Multiplexed editing of phage genomes using retrons, now published at @NatureBiotech with lots of new bells and whistles. @ChloeFishman @katecrawdaddy @BhattaraiKline @Darshini_Poola @thekarenzhang @alex_gonzadel @matromont
https://t.co/rkVtTEhVo1
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It has been an exciting and enlightening journey navigating my master’s project. Couldn't have been more grateful for the opportunity and mentorship 😇
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So excited to share one of the final works from my PhD in @SethShipman’s lab with @Asim_g_Khan and @oso_de_antojos, where we used high-throughput libraries and machine learning to create design rules for retron gene editors:
biorxiv.org
The bacterial retron reverse transcriptase system has served as an intracellular factory for single-stranded DNA in many biotechnological applications. In these technologies, a natural retron...
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An explainer/behind-the-scenes: https://t.co/XGJrQVA3x6 via @GladstoneInst.
gladstone.org
Scientists at Gladstone develop a more efficient way to make several, precise edits simultaneously to human cells
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Multitrons: simultaneous multi-site editing of individual genomes using retron arrays, now published in Nature Chemical Biology @nchembio: https://t.co/GlTCjsgLSg. Great job @alex_gonzadel and @oso_de_antojos !
New preprint! Simultaneous multi-site editing of individual genomes using retron arrays. We're trying to make it easier to make multiple, non-adjacent, precise mutations to a genome without long cycles of editing, isolating , and editing again.
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New Preprint, New Retrons! More than a hundred of them. We synthesized a ton of bioinformatically predicted retrons and put them through a battery of tests, from RT-DNA production to editing in human cells, E. coli, and phage https://t.co/6j08tzIkoD
@GladstoneInst @UCSF @UCSF_BTS
biorxiv.org
Retrons are bacterial immune systems that use reverse transcribed DNA as a detector of phage infection. They are also increasingly deployed as a component of biotechnology. For genome editing, for...
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Rebecca Fang from @seth_shipman's lab presents her work on using PacBio technology to sequence the mitochondrial genome. @GladstoneInst
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