Zhixing Chen
@ZhixingChen2
Followers
526
Following
754
Media
8
Statuses
146
Chemistry, Molecular Engineering, BioImaging, Biotechnology. Columbia/Stanford alumni. Asso. Prof. at College of Future Technology, Peking University
Beijing
Joined August 2020
Video abstract of PK Mito Orange prepared by our collaborator Dr. Till Stephan
PKMO: a new dye by @ZhixingChen2 that allows multi-color live-cell STED of #mitochondria. We´re so excited about this wonderful collaboration that we prepared a video to highlight some of the data recorded by @TianyanL and @stephan_till . 👇 https://t.co/trEnizQ5oF
1
3
28
Check it out at PNAS today. Thank you Alexandre for the collaboration!! PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by @JingLing369, Yitong Liu, and all the collaborators! Available @spirochrome
Thrilled to collaborate with @ZhixingChen2 Lab @PKU1898! We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
https://t.co/KLsx2cA5Fn
1
4
20
Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!! https://t.co/9QHbNAWZ5L
@spirochrome
1
15
57
Today we report the directed evolution of HUH (a sequence-sequence covalent protein tag for ssDNA labeling) into rHUH, a sequence-specific covalent protein tag for RNA labeling. rHUH was hard to engineer: the initial HUH template had no RNA activity, so we started with RNA-DNA
8
75
383
March 27 (Thu), 9:00–11:40 Speakers: Hiroyoshi Fujioka (Inst. Sci. Tokyo), Zhixing Chen (@ZhixingChen2, Peking Univ.), Satoshi Okada (Inst. Sci. Tokyo), Fan Zhang (Fudan Univ.), Hao Zhu (@HaoZhu10581033, Kyoto Univ.), Chu Wang (@ChuWangPKU, Peking Univ.) https://t.co/EmEA3EVgnJ
pub.confit.atlas.jp
Thu. Mar 27, 2025 9:00 AM - 11:40 AM JST The 105th CSJ Annual Meeting
1
1
2
My heart is broken as I announce the passing of my beloved husband, science partner and friend, Michael Sheetz. A remarkable scientist we packed 40 years of living into 20. Rejuvenation of senescent cells and mice in Press in Aging Cell; his last words were this was his best work
71
32
336
Happy New Year, everyone🍀 Excited to see one of my live-cell STED super-resolution microscopy images featured in this year’s @ibidiCells calendar! 🎉 Mitochondria stained by PKMO by @ZhixingChen2 and 610CP-CTX by @AndImaging , recorded on an @Abberior Expert Line.
2
1
31
How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling. https://t.co/rQyfAClwm1 Special thanks to Alice Ting for discussions!
0
15
78
Great collaborations between junior PIs😀PK mito dyes excel in applications beyond imaging and beyond traditional model animals!
I am thrilled to share our paper https://t.co/RPiuQpyvbp Mitochondrial dynamics govern whole-body regeneration through stem cell pluripotency and mitonuclear balance| Nature Communications great collaboration @Orangeroad2017 @ZhixingChen2
0
0
18
the department of polymer at Peking University is looking for 2 PIs at both junior and mid-career levels, all backgrounds are welcomed, but would prioritize those in polymer physics, functional soft matters, and AI for polym. sci. Pls contact me (chemhualu@pku.edu.cn) or RT.
0
2
7
I'm flattered everytime I see a multi-color movie with our gentle dyes together with mStayGold!
Time-lapse recording of floorplate cytonemes (green) interacting with axons (magenta). 1 image taken every second for 10 min (played at 30 fps). Cytonemes love interacting with axons! 💚#FluorescenceFriday
0
4
35
😍 PKmem 555 in action! Thanks for sharing!
'Ne me quitte pas'... Live imaging of two axonal growth cones meeting, interacting, and then separating. 🔬Can you spot the peculiar event happening between those two?🧐#neuroscience @UZH_Science
0
4
12
Our Gentle Rhodamines is finally published @ACSCentSci. See the healthy cytoskeleton free from photodamage even after 800 frames! 🦾💪 https://t.co/aN7gNl1iaV Great collaboration with Julian Kompa, the Johnsson group, and Spirochrome! @JulianKompa @spirochrome @TianyanL
2
16
63
A nice talk from Stefan in PKU, with a lot of mitochondria, superresolution, and some twists to cryoEM in the end . Then we ended the day in the Emperior's diner for dinner😀 @JakobsLab @ZhixingChen2 @xipeng1
1
3
38
🎉🍾10 YEARS - NEW WEBSITE! 🎂 Join us in celebrating Spirochrome's 10th anniversary and visit our new website! https://t.co/8BLjykwL78 10 years ago, the freshly founded Spirochome started commercializing its first two products, SiR-actin and SiR-tubulin! Today, with about 40
0
6
12
🚨Excited to share my latest postdoc work in this #biorxiv preprint on developing split-HaloTag recorders for capturing cellular transient kinase activity, Kinprola (kinase activity dependent protein labeling). https://t.co/iAUx1XG8gj 🧵 (1/7)
biorxiv.org
Protein kinases control most cellular processes and aberrant kinase activity is involved in numerous diseases. To investigate the link between specific kinase activities and cellular phenotypes in...
2
13
89
Nice work!
Since long time we tried to achieve “permanent” labelling of plasma membranes. Now it become possible with MemGraft probes, which exploit a new principle of lipid-directed covalent fluorescent labeling of membrane proteins. The MemGraft probes are compatible with fixation,
1
0
5
Very sad news. He was a pivotal contributor to the probes community and beyond.
Holy eff-ing F. Allan Waggoner passed😢 He invented CY3/5. I met him 2 decades ago when he told me that the CY dyes would change the world. I told everyone about CY5 - and was dismissed because “you can’t see it with your eye”
0
4
53
Glad to launch another collaboration with SpiroChrome! @spirochrome
🚨MORE NEW PRODUCTS🚨 The new and amazing "Gentle" Plasma Membrane (PM) PKmem™ probes from @ZhixingChen2 lab will be available at Spirochrome by the end of May. PKmem probes advantages: ✅Fast staining ✅Reduced phototoxicity ✅Robust and specific PM staining ✅SIM & #STED
0
1
18
Additional thanks to @aklymchenko @Mayeul_Collot and @TheBlanchardLab for their inspirational works on the magical membrane anchors and triplet-state quenchers.
0
0
4
Special thanks to @xipeng1 for the collaboration -- we are glorified to work together with top microscopists!
1
0
4