De-en Sun
@DeenSun3
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Postdoc in Kai Johnsson Lab @mpi_mr_hd @AvHStiftung @ResearchLifesci | Ph.D in @XingChen_PKU Lab @PKU1898 | Chemical biology, Expansion🔬, Chemigenetic probe
Heidelberg, Germany
Joined January 2018
Our biorxiv preprint is now online @nchembio 🎉.We made substantial revisions and added new experiments. Thanks to everyone who made this possible. Our journey continues!.
🚨Excited to share my latest postdoc work in this #biorxiv preprint on developing split-HaloTag recorders for capturing cellular transient kinase activity, Kinprola (kinase activity dependent protein labeling). 🧵 (1/7).
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RT @ShorterLab: Molecular recording of cellular protein kinase activity with chemical labeling:
nature.com
Nature Chemical Biology - Molecular recorders based on kinase activity-dependent protein labeling track specific kinase activities to understand their link to cellular phenotypes in heterogeneous...
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RT @hsi_yin: 🚨 New preprint alert!. Looking for a peptide tag alternative to the classic HaloTag for protein labeling? . Check out our late….
biorxiv.org
We introduce a high-affinity split-HaloTag comprised of a short peptide tag (Hpep, 14 residues) and a large, inactive fragment (cpHaloΔ3). Hpep binds to cpHaloΔ3 spontaneously with nanomolar affini...
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RT @jinzhanglab: Looking for a fluorescent biosensor and don’t know where to start? Want a comprehensive overview of the field? Check out o….
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Thanks especially to Kai Johnsson and to all involved! On X you will find @DirkCHoffmann @KimBoonekamp @Winkler_Lab @wick_wolfgang @Michael_Boutros @yulonglilab @mpi_mr_hd @EPFL_en @uniklinik_hd @UniHeidelberg @PKU1898 @AvHStiftung @ResearchLifesci (7/7).
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Kinprola enables the recording of PKA activation in primary neurons, acute brain slices upon drug or electrical stimulation, and for the tracking of drug-induced neuromodulation in freely moving mice🐭. (6/7).
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Kinprola enables the recording of differential PKA activities in heterogeneous cell cultures after pooled genetic perturbation🧬✂️, for genome-wide CRISPR screens to discover regulators of PKA signaling. (5/7).
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We applied Kinprola for capturing differential PKA activities within the heterogeneous network of glioblastoma cells, enabling to separate cells by FACS based on their kinase activity for RNA-Seq analysis. (4/7).
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The fluorescent mark can be chemically fixed and analyzed using flow cytometry or fluorescence microscopy🔬. We have designed specific recorders for four protein kinases, including protein kinase A. (3/7).
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Based on the split-HaloTag system we developed earlier this year ( and a phosphorylation-dependent molecular switch, Kinprola become rapidly labeled in the presence of a specific kinase activity and a fluorescent HaloTag substrate. (2/7).
science.org
Modified proteins allow recording of a cell’s regulatory activity at a specific period of time for later analysis.
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🚨Excited to share my latest postdoc work in this #biorxiv preprint on developing split-HaloTag recorders for capturing cellular transient kinase activity, Kinprola (kinase activity dependent protein labeling). 🧵 (1/7).
biorxiv.org
Protein kinases control most cellular processes and aberrant kinase activity is involved in numerous diseases. To investigate the link between specific kinase activities and cellular phenotypes in...
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RT @MichaelBollong: An inspiring read!. Synthesis at the Interface of Chemistry and Biology | Accounts of Chemical Research .
pubs.acs.org
ConspectusChemical synthesis as a tool to control the structure and properties of matter is at the heart of chemistry─from the synthesis of fine chemicals and polymers to drugs and solid-state...
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Glad to contribute to this work led by my phd lab @XingChen_PKU .Fast Metabolic Glycan Labeling for Click-Labeling and Imaging of Sialoglycans in Living Acute Brain Slices from Mice and Human Patients | Journal of the American Chemical Society
pubs.acs.org
Living acute brain slices provide a practical platform for imaging sialylation in human brain pathology. However, the limited lifespan of acute brain slices has impeded the use of metabolic glycan...
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RT @yulonglilab: 🔬Join us at #ChemoRevolution, where chemistry meets biology to reshape our understanding of life! . Explore cutting-edge t….
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RT @mpi_mr_hd: Our new #MPIMRSpotlight features @DeenSun3, postdoctoral researcher in the Chemical Biology department! ⚗️Check out what he….
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RT @mike_tilapia: Our paper on functional discovery of protein degradation and stabilization effectors with proteome-scale induced proximit….
nature.com
Nature - A synthetic proteome-scale strategy enables the identification of a diverse range of human proteins that can induce the degradation or stabilization of a target protein in a...
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RT @JulienHiblot: 🔬Ever wanted to spy on your model system? 🕵️♂️.Now you can! With bio-orthogonal chemical labeling, we've unlocked the ab….
science.org
Modified proteins allow recording of a cell’s regulatory activity at a specific period of time for later analysis.
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RT @vixklen: Out today in @ScienceMagazine: Seeing glycans attached to proteins and lipids - at the *single molecule level*! 🎉🎉🎉 . https://….
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RT @maxplanckpress: 😁🎉😄🌟#NobelPrize!🙃🙃🙃🙃🙃🙃🙃🙃🙃🙃🙃😊🏆😁#PhysicsNobelPrize2023 for Ferenc Krausz, Max Planck Institute of Quantum Optics! 🎊😃🥰😆🥳😁….
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