C²SD-ISM combines physical defocus rejection (spinning disk) with adaptive algorithm (DPA-PR), breaking depth limits in tissue super-resolution (↑180 μm), enhances fidelity (92% linear correlation), and supports high-throughput imaging. Github.

Congratulations to Suyi Zhong, Wei Ren, Ziyi Yang, and Hongyu Wang!

I have to re-tweet, as the previous one I used a different tinyurl and it cannot be seen by Altmetric. @altmetric.

Our groundbreaking Dark Sectioning is now published in Nature Methods! 🎉 It supercharges existing imaging systems: 📷Widefield → Confocal 📷 2D-SIM → 3D-SIM Now integrated in Airy PolarSIM and ! Matlab+Fiji pluin .@PKU1898.

RT @naturemethods: For a quick summary of the paper, read the Research Briefing here:.

RT @PKU1898: Watch the Story Video to the Review Article:. #PKUResearch #InnovateWithPKU @xipeng1.

Our groundbreaking Dark Sectioning is now published in Nature Methods! 🎉 It supercharges existing imaging systems: 🔬Widefield → Confocal 🔬 Single-photon → 2P 🔬 2D-SIM → 3D-SIM Now integrated in Airy PolarSIM and #FlexSIM! Matlab+Fiji pluin @PKU1898

Our latest long review explores the principles, advanced instrumentation, and cutting-edge applications of SIM in live-cell imaging. Both SIM (stripe-based) and ISM (point-based) are included with their derivatives. #Microscopy #SuperResolution

A Story of Envy,Jealousy,and Regret:autonomous driving is definitely the trend of the future.Once it is fully implemented,people might not need to buy their own cars anymore,and urban traffic problems would be instantly solved.The tide of technology is unstoppable.

🚀 A groundbreaking advancement in SIM! Xi group.@PKU1898.introduces a novel Fast Reconstruction-3DSIM @The_InnovationJ, accelerating reconstruction speed by up to 800-folds, paving the way for high-throughput, near real-time 3D super-resolution imaging.

🔬 We introduce MC-ISM, a groundbreaking #superresolution technique based on multi-confocal ISM, with 110 nm resolution, allowing continuous imaging of mitochondria for 1000 frames and imaging depth of 175 μm. @Natl_Sci_Rev

Our latest study published in eLight introduces a physics-rooted computational method to enhance fluorescence microscopic imaging. Our MRA deconvolution algorithms improve SNR by up to 10 dB and ensure fidelity, enabling more challenging imaging tasks.

RT @transpread: In the journal eLight, scientists developed a new method to improve resolution and reduce noise in fluorescence microscopy….

With MRA, we are aiming at providing an open-source alternative to Huygens deconvolution. 😀.

One day in Osaka, short but memoriful!

sCMOS is popular in fluorescence microscopy, yet its speed is limited by the exposure-readout process. Here we designed a parallel acquisition mechanism, to use galvo to project the image onto different ROIs of sCMOS, fully using the exposure-readout time.

RT @manorlaboratory: Gorgeous STED imaging of mitochondrial cristae and mtDNA 😍😍😍

HBmito Crimson unveils the mysterious interaction between mitochondrial cristae and mtDNA, with 39 nm STED resolution, >1.5 hour imaging, in healthy, apoptosis, and ferroptosis!

RT @ZhixingChen2: The MemBright PM stains set a high bar for specific membrane labeling. But if you care about the phototoxicity under tim….

Using polarized structured illumination +asymmetric mask, Prof. Peng Xi group achieved 3D super-resolution imaging of fluorescent dipoles. 3D SR imaging and dynamics of the milk fat globule membrane, λ-DNA, actin filaments, and microtubules expressing GFP.