Interested in building neuronal networks? Bioluminescence and optogenetics? Microfluidics? 👇

Thank you for having us! Looking forward to meeting you tomorrow!

And read the Research Briefing on this work, here: https://t.co/Mcee73BLAZ

In the PhAST technology, light serves as a synthetic neurotransmitter, by combining a calcium-dependent luciferase on pre-synapses with channelrhodopsin on post-synapses, which is illustrated in C. elegans. @NMSBlab https://t.co/PP9l5lBPv5

@wormsenseLab Tweetorial☝️. Uncut and final version now online 👇. Substantially improved presentation and more case examples of luciferases and channelrhodopsins with different colors, applied to two more circuits, the AWA olfactory and the egg laying circuit. https://t.co/qD62a0BQaQ

Finally, we push the system to obtain real time 3D reconstruction of low photon samples via light field microscopy by combining 2 neural networks to show that bioluminescent samples can be imaged and de-noised in 3D in a manner comparable to fluorescence microscopy ... 6/6

We employ deep learning to enhance the SNR of bioluminescence images in HeLa cells, mouse embryonic stem cell colonies, whole zebrafish embryos and whole C.Elegans body wall muscles and even single neurons ... 5/6

We show that bioluminescence microscopy evades the effects of phototoxicity during fluorescence imaging and heat using a transgenic worm (C. Elegans) which express reporters of cellular stress ... 4/6

We approach this by developing a new LowLiteScope, with ultracompact optical axis coupled to single photon resolving camera, to capture bioluminescent-tagged intracellular structures and whole animals with high SNR, comparable to fluorescence imaging ... 3/6

Bioluminescence is independent of external illumination that may perturb photosensitive cellular processes or produce autofluorescence. However, bioluminescent enzymes, such as luciferases, emit few photons and thus constrain the imaging of fast biological processes... 2/6

We are thrilled to share our story on deep learning- enhanced #bioluminescence #microscopy published in Comms biology! @CommsBio https://t.co/Hxl2s6v4wd A huge congrats to @LuiFelMorCur and all co-authors from @NMSBlab, @ICFOnians #SLNgroup and @CRGenomica ... 1/6

Very nice work and awesome collaboration. Looking forward to what comes next :)

We are hiring! Interested in doing your PhD in mitochondrial biology in Munich, and curious about how cells distinguish intact from mutant mtDNA? Please apply through our graduate school https://t.co/1U602Qld9n. #PhD #CellBiology #mitochondria

Great location, great people, great science.

If you are at Embl this week for #EESPhaseSeparation, stop by @neusanfeliu’s poster and learn about what large titin and stomatin homologs have to do with your favorite biological process and neuronal mechanotransduction.

Part 2 of @_mlechha's #TwitterTakever of @zhenlab_Toronto. As promised, here's a first thread about the history of the science of animal movement. 1/n

Great work by @DasraviDas et al. Check it out now in @ScienceAdvances

Fresh from the oven: I am very excited to share our recent paper. Special thanks and congratulations to everyone involved:@LuitgardLin @fedcat_131 @NawaphatMalaiw1 @neusanfeliu @Rabbs20 Aleksandra Pidde & Michael Krieg @NMSBlab @ICFOnians #mechanobiology

Congratulations to @DasraviDas @LuitgardLin @fedcat_131 @NawaphatMalaiw1 and all others involved. Thanks to everyone who helped and contributed in any way of funding, discussing and sharing reagents ... and now reading. #worms

3. proprioceptor activity peaks under neuronal compression in vivo (rather than stretch) 4. how K2P and TRP channel are involved to shape neuronal responses to steep membrane tension gradients (both positive and negative).