Really great to have our collaborative study with @Hepworth_Lab and @ClatworthyLab now published. In this study we developed our dynamic labelling of lymphoid tissue (Dutton et al., Sci Immunol. 2019) to now visualise immune cell migration into and out of tumours. 1/8

Relocating the lab to the Oxford Centre for Immuno-oncology going really well. Such a great research environment. We have new positions available, including this post-doc position: https://t.co/MfQSt0jJVS New address is: david.withers@immonc.ox.ac.uk - email to find out more

I am delighted to announce our recent Wellcome DA success with @ActonLab and @ClatworthyLab. Super exciting opportunity. If interested in joining our team as a post-doc please contact any of us for more info.

Great to see these two papers come out together today: https://t.co/yHLb7sPhWG https://t.co/ol2pPEAAOI Has been such a great collaboration with many groups, particularly the Clatworthy Lab. Congratulations again Colin and Isaac @ColinYCLee @ClatworthyLab

5 of 5 Key finding #3: Administration of IL15/IL-15ra caused distinct NK tumour resident state that maintained effector functions and appeared to enhance tumour control (Fig 6). Many thanks to all involved, particularly @IWD_Sci, @ColinYCLee, @ClatworthyLab

4 of 5 Key finding #2: Validation of transcriptomic changes confirmed really rapid changes in NK cells after tumour entry: upregulation of distinct integrins (e.g. CD49a) and loss of key effector functions (e.g. production of CCL5, IFNg) occurs after only 24hrs. See Fig 2/3.

3 of 5 Key finding #1: Using a combination of photoconversion and scRNA-seq, we show that conventional NK cells rapidly adapt their transcriptome following entry into the tumour, establishing a distinct tumour-retained/resident state in only 48-72 hrs. See Fig 1.

2 of 5 Building on the approaches published last year ( https://t.co/5AbdenOpZY), here we have interrogated what happens to NK cells after tumor entry and for the first time provide a real-time map of their progression to a dysfunctional state.

1 of 5. We are pleased to present our latest study using our dynamic tumour-labelling models:

We have a post-doc position available focused on intestinal and cancer immunology. Ideal for PhD students soon to submit, who want to work with some cool in vivo models to better understand immune responses. Position advertised on BSI Jobs Board https://t.co/aF4sGYds8s

3 year Post-doctoral position in the Withers Lab at the University of Birmingham Details: https://t.co/yhHg1SsXHs For more information and/or informal discussions regarding the post, please contact: d.withers@bham.ac.uk

We are thrilled to announce the speaker of our next i3 @DKFZ seminar: on November 14th at 4 pm we'll have the honour to host Prof. David Withers from @unibirmingham who will talk about a novel strategy to track changes in TIL function upon migration into tumors. Save the date! 🗓️

Pleased to see our paper "Photoconvertible tumors to track T cell dynamics" with my former lab @LabWithers is featured in a special collection on Mechanisms & Models of Cancer @JExpMed @cshlmeetings See the special collection to accompany the meeting:

So great to see this finally out!! Top team @ClatworthyLab @loudon_kevin @KelvinTuong @drbstewart @iScience_CP https://t.co/jSXkoHVPE7

Very excited to share our new work explaining how #antibiotics can lead to life-threatening fungal infections, out today in @cellhostmicrobe! A short thread on the main take-home messages... 1/10 @LionakisLab @jigarvdesai https://t.co/RjXDqvF0sN

Pleased to have my first publication with @ClatworthyLab out & hopefully many more to come! Read our work in @FrontImmunol here:

.@ZhiLiImmunology @KelvinTuong @IWD_Sci et al use photoconversion to temporally label tumor-infiltrating lymphocytes, revealing the continuous migration of TCF-1+ T cells btwn the tumor & draining lymphoid tissue https://t.co/KoJkmsuv0F @LabWithers @ClatworthyLab @Hepworth_Lab

Many congratulations @ZhiLiImmunology, @KelvinTuong, and @IWD_Sci on our first (of many 🤞) tumour immunology research outputs. https://t.co/CQnnR2PPIx

Collectively, our data reveal new insight into the dynamic nature of different CD8 T cell subsets within tumours. Huge thanks to all collaborators and funders of this project @wellcometrust @CancerResearch @WorldwideCancer @AstraZeneca 8/8

Finally, we asked determined that blocking PD-L1 resulted in reinvigoration of retained exhausted cells as well as the enhanced responsiveness of newly recruited CD8 T cells upon entering the TME. 7/8

We further discovered that the absence of TCF-1+ PD-1+ CD8 T cells from the tumour resident population was explained by their egress back to lymphoid tissues. Thus the stem-lie niche appears highly dynamic, balancing constant recruitment with escape from the TME. 6/8