FPbase
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The Fluorescent Protein Database. tweets by @TalleyJLambert
Joined January 2019
Try the new FPbase spectra viewer! . Completely rewritten to be faster, more customizable, and full-featured:. • SVG/PNG/CSV export.• state recovery & URL sharing.• keyboard shortcuts for rapid entry 💚.• efficiency calculations.• full documentation.
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RT @JamesDManton: This exercise was less informative than I'd hoped, but perhaps it's interesting to someone else: spectroscopically-inferr….
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RT @IvanMasl0v: Mutagenesis of #FbFP #LOV domain:. 🟠 22 variants with 486-512 nm emission maxima. 🟠 three-channel imaging via spectral unmi….
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RT @lysergicdesigns: Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLante….
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Chapter on using FPbase tools out now in the MMB issue on FPs. Thank you for editing @mayankdu!.
Happy to share the Methods in Molecular Biology issue on Fluorescent Proteins 🌈🎉😀. Including many “much awaited” protocols on and about Fluorescent Proteins/applications. A big THANKS to authors across the globe for contributing. 👏🏻💐.
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RT @aandr314: this is genius: @helsinkiuni BioImaging Unit utilizes @FPbase to allow users test their fluorophores. .
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anyone out there routinely express FPs in Chlamydomonas? any modern suggestions for SNR? (presumably red, but any expression gotchas?).
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RT @eszter_cs: 🥁 Delighted to announce that our FPCountR paper is now on bioRxiv! . In this paper we describe the development of a method t….
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Always nice to see features put to use!. @eszter_cs and @gbstan use the FPbase API (among other things) in a method for the calibration of fluorescence readings on microplate readers; for quantification of FP concentration.
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RT @miCHRIScopy: Just discovered the interactive chart of Wow, what an amazing tool to compare different propertie….
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RT @ratherbecloning: @miCHRIScopy @TalleyJLambert FPbase is amazing! I created scope configurations for @UAZCancer core instruments, and li….
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Writing a methods chapter on using FPbase for an upcoming MMB book. Will include things like. -Browsing/searching FPs on fpbase.-Spectra viewer.-microscope & reports feature. If there's an aspect or detail of FPbase that you wish were better documented, let me know!.
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This is beautiful and makes me want to rewrite FPbase using it 😍.
Big project, first public release! 📢. ✨ SQLModel ✨. the biggest thing I've built since FastAPI & Typer. 🤓. SQL DBs based on Python type hints. 😎. Each model is both a Pydantic and @sqlalchemy model. 🤯. Optimized for FastAPI 🚀.
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Dear imaging companies,.Please tell your customers (or make available on request) the *actual* spectra for all components (LEDs, filters, etc) in their light path. just saying "460-490nm" is not sufficient. It's amazing how many scientists can't get this info from their reps 😞.
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a reminder on the difficulty in comparing fluorescence photobleaching measurements across studies (and even across probes on the same scope).
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Databases are hard. and no amount of automated error checking can catch every mistake that humans make (in the literature or in submissions). FPbase gratefully accepts help/corrections from anyone!. Here's an example of what not to do if you find something needing correction 😂
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RT @TalleyJLambert: @rhodamine110 @Merz @edward_marcotte @J_A_C_S more JF spectra than you can shake a stick at. (2P coming soon). thanks @….
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