An interesting alternative to iterative deconvolution — just sample from the posterior and average the samples!.

RT @jmrko: Interested in working on a state-of-the-art connectomics pipeline at its birthplace as a postdoc? We’d love to hear from you! :)….

RT @jgreener64: I have two job adverts open at the minute:. - A PhD position on fast MLIPs for biomolecules (. - A….

RT @nilnorl: Two available postdoc scholarships at Norlin Lab in Lund!.1. Optical Engineering: OPM/light-sheet microscopy and lensless imag….

A nice use of multispectral microscopy to study the organelle signatures of neurons and astrocytes in 2D+t and 3D! I look forward to playing with the data they've generously made available at

Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all:. Feedback always welcome.

RT @SuperResoluSian: If you find these kind of incisive scientific discoveries - fluorescence microscopy is not cat photos - interesting th….

And if we try milling some flat glass, we see interesting undulations in the milled surface. If any of us ever form a band, we all thought this would make a good album cover. 8/8

We also see nice contrast with the tiny gold wires that connect the sensor chip to the main board. The ion beam showed good contrast over most of the board, which gives us confidence that it'll be a good tool for inspecting chips after some semi-destructive modification. 7/8

If we repeat the process, but offset the milling by a fraction of a pixel, we see a different cut through the electronics. 6/8

Yep, definitely electronics. 5/8

Rotating the view by ~90 degrees shows some interesting structures, which are presumably to do with the electronics of the chip. 4/8

"I wonder if we mill a trench if we'll see anything interesting underneath. ". We've definitely made a hole, but it's not clear that we've learnt anything. Maybe if we rotate the view we'll see something. 3/8

The SEM image showed very low contrast, but imaging with the ion beam on the Scios clearly showed the microlenses, which is what we were originally looking for. These have the expected 3.45 μm pitch. 2/8

For science, Jacob Lamb, Hugh Wilson and I had a fun morning using a focussed ion beam to image a @Thorlabs CS165MU/M camera. Here's the board loaded into the sample chamber. 1/8

RT @Zhang_Suyang: Our lab @MRC_LMB is seeking a passionate PhD student to explore transcription-coupled splicing! Join our team for an exci….

Thanks to @royalsociety and @MRC_LMB, @lab_baum and I are looking for a student to develop the next generation of fluorescent proteins for live and super-resolution imaging, as part of the LMB's four-year PhD programme.

Congratulations to @_Akaash_Kumar for being awarded the Royal Microscopical Society's Early Career Award for his PhD work on multispectral imaging!.