🚨Why can’t mammals regenerate legs or arms like frog tadpoles or salamanders? .Our preprint tackles (part of) this BIG question with surprising findings about oxygen sensing.🔗Happy New Year, everyone! 🎉.🐭 vs 🐸 #evodevo #regenerative #cellbio 🧵👇.

RT @Jain_Akanksha_: Very happy to share it’s online! If you read the bioRxiv, updates include tracking cells and tissue flow, Histo4i tim….

RT @naturemethods: A nice advance for imaging-based spatially resolved transcriptomics from the Weigert and La Manno labs. Spotiflow uses d….

RT @zhumeng123: Very honored and grateful to be named one of the 100 extraordinary biologists!It’s been a joy working with the @Co_Biologis….

RT @Hallou_Lab: 🚨 Paper Alert 🚨 .Absolutely delighted to share our last paper on "spatial mechano-transcriptomics" in @naturemethods👇!!.htt….

RT @AlejandroAgCa: Hiring alert! @SRsrivatsan @fredhutch and I @HHMIJanelia are looking for a joint postdoc to combine single-cell omics an….

Still an MPI für *Entwicklungsbiologie* kid at heart, but now calling FML home! #LabMove #NewNameSameGene

Modern tools challenge long-held assumptions - interesting findings on local oxygen levels during fracture repair! 👇 The plot thickens with the entry of 🩸 :).

RT @epflSV: 🚀 Calling early-career scientists! Apply for the 2025 ELISIR Scholar at EPFL. From outstanding PhD straight to group leader. Jo….

RT @MekaylaStorer: We are really proud to share the first 'Pre-Print' from the Storer lab driven by the fantastic @Byron_Mui, and co-lead b….

RT @frogwranglers: Check out this cool new preprint on limb regeneration and oxygen sensing using #xenopus! @CanAztekin. .

10/ Interested in the evolution of regen, why mammals are bad at it, and how environmental factors influence regen across species? Want to delve into single-cell methods with new simplified cultures?. Join us @MPI_Bio /FML for the next discoveries! 🚀.Our positions are still open.

9/ Huge thanks to our amazing team/collabs -@GeorgeTsissios, @EpiGN_Lab, @mssakar, and more-, @epflSV facilities, and funding agencies. We’d love your feedback before journal submission—DMs and emails are welcome! 📩.

8/ Species-specific oxygen sensing lies at the heart of regeneration initiation— and its influence goes beyond: from morphogenesis to cell mechanics, epigenetics, cross-species traits, and evolution. —there’s something here for everyone!. one of my fav pics from the paper 👇

7/ So, regenerative animals have a double advantage: environmental + genetic, predisposing them to this incredible ability. This discovery opens the door to targeting mammalian regenerative potential—at least its initiation.

6/ How is this possible?.We found out that species sense oxygen differently!. Certain genes allow cells to sense oxygen levels (2019 Nobel Prize! 🪙), but this machinery seems to be less active in 🐸and axolotls, while highly active in 🐭 and humans 😯.

5/ But things got even more interesting 🤔.Our data indicated atmospheric oxygen would block regeneration. However, when we challenged frog tadpoles with higher oxygen levels (>60% O2), they STILL initiated regen just fine! 🐸💪.

4/ Lowering oxygen levels 🌬️ induced wound healing, altered cell mechanics and the epigenome—altogether permitting regeneration initiation in mouse limbs!. Most regen animals live underwater (low oxygen), while non-regen ones dominate on land—more than just a correlation?.

3/ It was exciting to see how the type of plate used caused such a big phenotype change!. This raises concerns for many studies—similar issues have been reported in simpler settings (check out this beautiful work which refers to earlier findings too: 🔗).

2/ Surprisingly… they could! 🤯 But only in 96-well plates 🧫. Of course, 96-well plates aren’t magical (SHOCKING)— they had sub-atmospheric low oxygen levels (not hypoxic!), unlike what we terrestrials live in, leading to this phenotype🌬️.

1/ Our previous work showed: (1) Frog tadpole limbs can initiate regen in a plate. (2) tadpoles use regenerative cells also present during mammalian limb development So, can mammalian limbs also initiate regeneration in a plate? 🤔.