Happy to share our new work where we took a deep dive into ER-mito contact sites and metabolic cell bio! Thanks to all the authors and most importantly @YatrikShahLab and @LyssiotisLab for their support. I will share some snippets of this journey from my perspective (🧵👇) (1/n)

Amazing resource paper in @Cell_Metabolism from @PeteMullen @LyssiotisLab & colleagues! A metabolic atlas of mouse aging https://t.co/5FypcZeknr Metabolic aging across 12 organs and five time points, with cross species validation of depleted hydroxyproline.

Big congrats to @KivancBirsoy and @junyue_cao, who have both been promoted! https://t.co/LQo9V3Zi3G

Thank you for the shoutout! We hope our platform will be useful for future deorphanization efforts.

Cover article of @Cell_Metabolism by @KivancBirsoy @RockefellerUniv is a fitting tribute to NYC skyline & our subway map! Machine-learning-guided discovery of SLC25A45 as a mediator of mitochondrial methylated amino acid import and carnitine synthesis https://t.co/doZDdX2Wd9

Iron addicted colorectal cancers exploit Heme-Complex II axis to resist oxidative cell death https://t.co/puT2oF8R33 #biorxiv_cancer

Also thanks to Zachary Sebo and Navdeep Chandel for writing a preview to our work as well as a complementary study from Thomas MacVicar's lab

The link to our paper:

The cover depicts a GWAS Manhattan plot as a city skyline reflected onto a New York City-style subway metabolic map: organelles as boroughs, metabolites as stations, transporters as trains.

Happy to see our recent work on SLC25A45 featured on the cover of @Cell_Metabolism. Huge thanks to @YuyangLiu1996 for the design! @KivancBirsoy @RockefellerUniv

Looking for suggestion for treating cells with phospholipids and lysophospholipids in increase levels of specific phospholipid species (PC/PE/LPC/LPE)? Liposome? alpha-cyclodextrin? Just in ethanol? liposome + cyclodextrin? 🤨🤔 #lipidtime #AcademicTwitter

New! Online now: Machine-learning-guided discovery of SLC25A45 as a mediator of mitochondrial methylated amino acid import and carnitine synthesis https://t.co/TrOQBQCiTO

@Cell_Metabolism @ArtemAKhan @RockefellerUniv Complementary work by Tom MacVicar in @MolecularCell

Our new work in @Cell_Metabolism by @ArtemAKhan describes three new SLC transporters, including SLC25A45, a mitochondrial transporter for methylated amino acids that is essential for carnitine synthesis and fasting adaptation. @RockefellerUniv

Check out my labmate @ArtemAKhan’s work! Glad to be part of this scientific journey. See below👇

I would like to thank @KivancBirsoy for the mentorship as well as all the authors who made this work possible @frederick_yen @nicoledelgaudio @GokhanUnlu_ RanyaErdal @michaeljxiao KhandoWangu KevinCho @egamazon @gjpattij

I also would like highlight two recent works on SLC45A4 https://t.co/OKOQoJziRr https://t.co/KEBJLvSXnp well as a complementary work on SLC25A45 https://t.co/Y4hOXG6iJI.

Biochemically, SLC25A45 is necessary for the transport of methylated basic amino acids, such as trimethyllysine, and also ADMA.

We then showed that knockout of Slc25a45 in mice leads to an impaired carnitine biosynthesis which becomes limiting under fasting conditions, compromising β-oxidation and energy homeostasis.

We found SLC25A45 was significantly associated with trimethyllysine, a byproduct of protein catabolism and a precursor for carnitine biosynthesis.

Here, we used a machine-learning-guided approach to uncover the function of three transporters: UNC93A and SLC45A4 as mediators of plasma membrane acetyglucosamine and polyamines transport, respectively, and SLC25A45 as a mediator of mitochondrial methylated amino acid import.