Excited to finally see our paper on KIR3DL3 out now in @SciImmunology. An answer to the question, “where is this receptor hiding?” https://t.co/J6MaNElKm5

Don't like p-values? We're presenting a completely new interpretation in the context of clinical trials. With Andrew Gelman, Sander Greenland, @guido_imbens Simon Schwab and Steve Goodman. @Lester_Domes @goodmanmetrics @NEJMEvidence https://t.co/iIu46aY3mZ

50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells https://t.co/qA5h4digkE #biorxiv_immuno

Thymic eviction explains CD8 T cell tolerance and why autoreactive CD8 T cells appear in the periphery but not the thymus @ScienceMagazine Badr Singer https://t.co/NpVFcyGAYX

🔥🚨@TargetALS funded TDP-43 RNA aptamer preprint🚨🔥 Using deeply phenotyped #ALS post-mortem tissue cohorts we show nuclear TDP-43 pathology is an early event, co-incident with STMN-2 cryptic splicing, preceding cytoplasmic aggreg. & symptom onset 🧵1/4

What gets erased when you integrate #singlecell data across samples/studies, and can you get it back? When samples are from e.g. healthy & disease, should you simply massage cells together? FINALLY, I feel we can answer this question: https://t.co/0PrkIZDyDp

A mass screening of immune activating receptors on natural killer cells shows how different peptide variants can affect innate immunity. @mjwsim @NIAIDNews Check out the study: https://t.co/kMWthAqhE4

I’m excited to share some work I co-lead with @i_rodcast and spanned the labs of @PardisSabeti, @ReillyLikesIt, and @r_tewhey to design and validate synthetic cis-regulatory elements with cell type-specific activity. https://t.co/BqZYaGKwJX 1/n

Absolutely delighted to share our paper detailing an HLA association with asymptomatic SARS-CoV-2 infection, out today in @Nature @UCSF with @GrasLab @dan_gard @jsabatino37 @martinmaiers @spellman_s @LawtonMurdolo @DimitraChatzil1 https://t.co/7xFcG7lIMj

Researchers @BillyHPalmer and @PN0rmski from @CUBiomedInfo report their investigation of a potential new immune checkpoint target (KIR3DL3/HHLA2) - work which could inform new approaches cancer treatment. https://t.co/sWbElnxZaj

Are you or do you know an evolutionary biologist working non-in-academia? See below, and please retweet! Link to survey: https://t.co/ljXKuVDmzN.

Congratulations @popgen_oumie ! What viruses do lab flies commonly have, and what might this do to gene expression?

🎉Exciting Update of scGPT 🎉: After receiving significant attention from the community since our April release, we're thrilled to announce the first major update for scGPT - a foundation model for single-cell multi-omic data. This update integrates community feedback and

Such a fun project to work on with contributions from so many. Special shout out to Norman lab members who worked on this @laleaton @anaacodo Liyen Loh @PN0rmski

HHLA2 has already garnered interest as a therapeutic target. Our results suggest that KIR3DL3 is an interesting candidate for immune checkpoint blockade due to its tissue-specificity, its association with a unique set of TCRs, and its role as an inhibitory receptor.

We also confirm that KIR3DL3 recruits SHPs and binds HHLA2 as a ligand to inhibit immune activation (eg. NFAT luciferase below). Because KIR3DL3 is very polymorphic, we also tested the functional impact of genetic variants. SNPs did not change HHLA2 binding but altered expression

These KIR3DL3+ T cells had a unique TCR repertoire – biased towards Vδ1 and early-rearranging TCRα chains. Together, the expression profile and TCR attributes of KIR3DL3+ T cells are consistent with the idea that these cells may have autoreactive properties

scRNA-seq showed KIR3DL3 marked a population of T cells with signatures of recent T cell activation and hypo-functionality. Follow-up flow cytometry and functional experiments helped confirm these findings.

We trawled through >300,000 RNA-seq samples and looked at protein in 5 tissues. KIR3DL3 expression is rare in peripheral blood but much more common in gut-resident immune cells. While most KIR are expressed in NK cells, KIR3DL3 expression was primarily in T cells, not NKs.

🐓 Key gene that blocks the spillover of #AvianFlu to humans discovered. @RuteMPinto, @WilsonLabCVR & colleagues have identified that the BTN3A3 gene is vital to protecting humans against avian flu. 📄 Paper: https://t.co/p98oaieDg7 🗞️ News article: https://t.co/N2KjF9EvYK