We are excited to share our latest work on the cooperation between RNA polymerase and the ribosome in bacteria: https://t.co/Ni4nJGJAug The work was supported by @IGBMC, @Inserm, @CNRS, @unistra, @ERC_research, @IMC_Bio, and @instructhub. 1/7

Max Planck President Patrick Cramer @mpgpresident has written an open letter to the President of @Harvard, expressing his full support to the University & its stance against demands from the Trump administration to change its policies in order to retain federal funding

We are pleased to share our work on the evolution of translation initiation now published in Nature Communications. Take a look at leaderless mRNAs and eS26 in the small ribosomal subunit of Saccharolobus solfataricus. https://t.co/6g5bFuV2r3

Please spread the word: we are looking for PhD students! Interested in supramolecular complexes in gene expression? Go to https://t.co/xMLfPjGxSh scroll down to “Integrated Structural Biology (LabEx INRT)” for project descriptions and start application process by January 19th!

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @MarkertJon https://t.co/g27t5Rw5RY

First paper of our lab today @Nature by Nussi! We provide a real-time movie on how #transcription and #translation cooperate using multi-color #single-molecule FM: we find long-range #ribosome-#polymerase communication mediated by #RNA looping! @embl https://t.co/ui1h01VaQW

We want to thank additional people involved: Kristine Charles, Nataliya Miropolskaya, Maria Takacs, Charlotte Saint-André, Alexandre Durand @DurandA17996106, Nils Marechal @marechal_nils, @BioS_ME_Alsace, Gregory Effantin, and Eaazhisai Kandiah at beamline CM01 @esrfsynchrotron

Closely related work has also been done in the laboratory of Shun-ichi Sekine: https://t.co/kSRjp77HAy 7/7

Andrea Graziadei in the @RappsilberLab used in vivo crosslinking followed by #MassSpec. The results are consistent with our #cryoEM results and suggest RNA polymerase may first approach the 30S via bS1 and be tethered to uS10 by NusG in a transcribing-translating expressome. 6/7

We teamed up with @AdrienChauvier in the @NilsWalterLab, who used single-molecule co-localization to show that bS1 and RNA polymerase together accelerate recruitment of the 30S subunit to the nascent mRNA. 5/7

Alternatively, RNA polymerase can also be tethered to ribosomal protein uS10 by transcription factor NusG. In this case RNA polymerase is able to feed the mRNA through the mRNA entry channel to the 30S. 4/7

Using #cryoEM, @Huma_ribosome and @WebsterMichael0 visualized multiple states revealing how the emerging, nascent mRNA first binds to ribosomal protein bS1 and is then guided to bind the anti-Shine-Dalgarno sequence, entering through the 30S mRNA exit channel. 3/7

In prokaryotes, transcription and translation are coupled. However, it is unclear how the small ribosomal subunit (30S) is recruited to the nascent mRNA emerging from a transcribing RNA polymerase and how coupling between RNA polymerase and the 30S is established. 2/7

https://t.co/BxZF9bdO6E

Excited to share a pre-print by Vita Vidmar and @borde_celine in collaboration with @OlivierEspeli and @valeroche about the interplay of #Transcription and DNA topology: https://t.co/IiiUOKaGHe

Wow! Historic!

Structures of transcription-translation coupling complexes at each stage of the translation cycle (Collaboration between our laboratory and the laboratories of Yue Gao, Jinzhong Lin, and Chengyaun Wang. Extensively expanded preprint. Posted 07/25/24.) https://t.co/zKAUiRj4bH

Special thanks to collaborators: @ClemCharLab and @CianferaniS

The project would not be possible without the previous and generous support from the @Fondation_BS and the University of Strasbourg Institute of Advanced Study ( https://t.co/cJlJG6aDZ4).