Rupert Faraway
@rupert_faraway
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RNA biology. Postdoc in the Plaschka and Ameres labs. Formerly Ule lab.
Vienna, Austria
Joined September 2017
We are very excited to share our recent preprint on the nuclear import mechanism of the proteasome, driven by the multivalent adaptor AKIRIN2! https://t.co/dJdiL7RAK8 🧵 (1/7)
biorxiv.org
Nuclear protein homeostasis, including the turnover of transcription factors, critically depends on nuclear proteasomes. After each cell division, proteasomes need to be re-imported into the newly...
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Come and explore the mechanisms of gene expression with us! We are hiring two postdocs in cryo-EM and - with @BalzarottiFran - in super-resolution microscopy. 👉 https://t.co/ipeZ4iQigE 👉 https://t.co/XXlCLZUdBC
imp.ac.at
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Our paper on cryptic splicing-controlled protein expression, which we hope will inspire safer and more adventurous gene therapy approaches for ALS and FTD, has been published in @ScienceMagazine
science.org
Loss of function of the RNA-binding protein TDP-43 (TDP-LOF) is a hallmark of amyotrophic lateral sclerosis (ALS) and other neurodegenerative disorders. Here we describe TDP-REG, which exploits the...
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How can cellular signalling swiftly & selectively remodel the transcriptome to drive biological transition? How this switch is orchestrated has been a mystery... 🧵 https://t.co/3z4dcVoztD
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The next interview in our series "IMPloyable talent" features Farja Ayala Muñoz, recent PhD graduate from the @plaschka_lab. Farja is a skilled biochemist, #opentowork in Vienna, & seeking industry roles. Get to know her: https://t.co/uimXyD2KTG
@viennabiocenter, @TrainingVbc
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We are excited to share our latest insights into human mRNA export. Pre-print: https://t.co/TCO75EW1f3; tweetorials below and https://t.co/824pXYIDgl by the exceptional @HohmannUlrich, who led the project in a joint effort of the @plaschka_lab and @JuliusBrennecke labs. (1/7)
I’m beyond excited to present my postdoc work with @plaschka_lab and @JuliusBrennecke at @IMPvienna @IMBA_Vienna . We uncovered a mechanistic framework for a general and conserved mRNA nuclear export pathway. A thread: 1/x
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Opportunity of a lifetime! Apply immediately.
We're looking for someone with a creative mindset and excellent modern molecular biology skills (DNA manipulation, Illumina, Nanopore etc) to join our team at @ucl and @TheCrick in the @FrattaLab developing candidate gene therapies for ALS. Apply here:
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We're looking for someone with a creative mindset and excellent modern molecular biology skills (DNA manipulation, Illumina, Nanopore etc) to join our team at @ucl and @TheCrick in the @FrattaLab developing candidate gene therapies for ALS. Apply here:
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Flora is an awesome scientist! You should work with her.
We are hiring an RA! Are you interested in mRNA localisation and local translation, presynaptic function and/or neurodevelopmental disorders? More details in the link, deadline is 14th March. Please retweet!
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Can MS2 loops stall reverse transcription? Does anyone know? I'm using Superscript IV at 50C.
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My lab @MDC_Berlin is looking for a PhD student (fully funded) in RNA biology and data science, to investigate how oxygen regulates the formation of phase-separated condensates in the cell, and controls physiological responses!! #PhDposition
https://t.co/Q2HOONEWj9
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New BioRxiv paper on using cryptic splicing to unlock precision gene therapy activation for ALS, FTD & other TDP-opathies! Key contributions @MaxZYJChien Josette Wlaschin +many others from @ClaireLePichon @ule_lab @FrattaLab labs and more. A thread 🧵 1/13
biorxiv.org
A system enabling the expression of therapeutic proteins specifically in diseased cells would be transformative, providing greatly increased safety and the possibility of pre-emptive treatment. Here...
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thought I ought to make a couple of xeets(?!) about https://t.co/cQ5VEtLaBL! a project >10 years in the making, now greatly expanded and improved by @goodwright_ & the advent of @nextflowio and @nf_core
https://t.co/1U3jP8XulG
biorxiv.org
Ever-increasing volumes of sequencing data offer potential for large-scale meta-analyses to address significant biological questions. However, challenges such as insufficient data processing inform...
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Also check out this great pre-print from Alexa and Adrian in the Pelkmans lab on more global mechanisms for mRNA retention in the nuclear speckle! https://t.co/uetYcFO4KX
biorxiv.org
Nuclear speckles are membraneless organelles that associate with active transcription sites and participate in post-transcriptional mRNA processing. During the cell cycle, nuclear speckles dissolve...
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A huge thank you to all authors involved in the study: Neve, Holly, Oscar, Nobby, Ira, Lea, Clemens, Stefan and most of all Jernej! I’m happy to answer any questions about the work.
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We identified one example of this mechanism, but it remains to be seen whether others also exist. Other types of LCD have their own multivalent binding partners. We are also excited to learn more about the cellular and disease contexts in which interstasis is perturbed.
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Interstasis represents a novel mechanism for the mutual homeostasis of related classes of LCDs at the RNA level. Multivalent RBP assemblies exploit the genetic code to predict features of the encoded protein from the RNA, and LCD dosage alters condensation to bias the RNA’s fate.
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Finally, interstasis of charged LCDs can be modulated by CLK kinases, which phosphorylate SR proteins. CLK kinases are highly regulated during cell cycle progression and in response to changes in temperature. Interstasis of charged LCDs may also be regulated in these conditions.
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