🎉 Incredibly proud to share my first-author paper in @Nature! Heartfelt thanks to my mentor @AndrzejDziembo1 and the brilliant team at @DziembowskiLab. More details in the 🧵below ⬇️

RT @gaworj: The second Pole in space in our Polish history. 47 years after the flight of a Polish soldier, we have a scientist in space. 🚀….

RT @MissAgataStar: Rekrutujemy! Doktorat u mnie w labie, w projekcie i pod opieką Olgi Iwańskiej w ramach Sonata NCN

RT @LOkruszek: Gdyby media poświęcały regularnie tyle uwagi walce o finansowanie nauki, #3ProcentNaNaukę, #NCNtoTlen i sukcesach polskich n….

RT @IIMCB_Poland: 🔬 Join us for a full-day event at IIMCB in collaboration with @thermofisher. Discover the power of Glacios 2 Cryo-TEM & H….

RT @IIMCB_Poland: Scientists from the IIMCB described a new mechanism that improves the efficiency of mRNA-based therapies. The research fi….

RT @hemrams: @Nature Fascinating work, @Nature! TENT5A re-adenylation adding a new layer to mRNA vaccine optimization is a big deal—especia….

RT @Nature: Nature research paper: Re-adenylation by TENT5A enhances efficacy of SARS-CoV-2 mRNA vaccines.

RT @kawecki_maciej: I to jest wielkie! Same polskie nazwiska na łamach najważniejszego czasopisma naukowego na Ziemi, Nature! Badacze z Mię….

I'm grateful for this milestone in my career - an experience that’s shaped me both scientifically and personally. Looking ahead with excitement to the next chapter and new opportunities to grow.

🙏 Huge thanks to everyone involved—our incredible team (@DziembowskiLab), collaborators (@UniWarszawski #WUM, @IBBPAS), and funding agencies (@ERC_Research @NCN_PL @FNP_org_pl @PORT_Wroclaw). (20/n).

🇵🇱 Notably, this represents a landmark achievement as likely the first @Nature article of the 21st century conducted entirely in #Polish laboratories! (19/n) #PolishScience.

This publication represents the culmination of a long,challenging journey.We initially posted our findings as a #preprint on @biorxivpreprint in December 2022.The rigorous review process demanded additional experiments,which significantly strengthened our final manuscript.(18/n).

These findings have immediate implications for therapeutic mRNA design: optimization of ER-targeting signals could enhance TENT5A-mediated re-adenylation, potentially improving vaccine efficacy and reducing dosage requirements. (17/n).

Notably, this effect was specific to #mRNA vaccines—#TENT5A deficiency did not impair antibody production in response to the protein-based #vaccine #Nuvaxovid (@Novavax). (16/n).

🐭 In vivo studies showed that TENT5A-deficient mice produced significantly less antigen-specific #antibodies following #mRNA #vaccination. This highlights the essential role of #TENT5A in mounting an effective immune response to mRNA-based vaccines. (15/n).

Our findings reveal a mechanistic principle: spatial proximity to ER-resident #TENT5A governs re-adenylation efficiency. This positions the #endoplasmic #reticulum as a central hub not only for #mRNA translation, but also for post-transcriptional regulation. (14/n).

Surprisingly, comparative analysis revealed differential re-adenylation efficiency between vaccine formulations: @BioNTech_Group -@pfizer #BNT162b2 demonstrated reduced re-adenylation compared to mRNA-1273, correlating with diminished #ER membrane association. (13/n).

The re-adenylation phenomenon exhibits substrate specificity - consistently observed in synthetic mRNAs encoding proteins targeted to the #endoplasmic #reticulum (ER), including #spike #glycoprotein, #Zika virus antigens, and #malaria parasitic proteins. (12/n).

We found that #macrophages—not dendritic cells—are the primary cells that take up mRNA vaccine #LNPs and express #TENT5A, positioning them as key players in regulating mRNA stability via re-adenylation. (11/n).

Of note, recent work from our lab (by @nemitheasura) showed that TENT5A incorporates non-adenosines into poly(A) tails of mRNA-1273, further underscoring its role in shaping the fate of therapeutic mRNAs. 👉 (10/n).