Joseph Kim
@jykim35
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Postdoc at UCSF | PhD from UW-Madison | Cryo-EM and its applications to drug discovery and neuroscience. Brunch, Sugar, Triathlons, Video Games, and Museums
San Francisco, California
Joined December 2018
Me: *Minding my own business* Liquid Nitrogen Tank Pressure Relief Valve: ALLOW ME TO SING YOU THE SONG OF MY PEOPLE.
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Well done Lauren! It was a privilege to work with you when I was at Madison!
Very excited to share a preprint of my thesis work examining the form of the F-BAR proteins CIP4 and FBP17 in neuronal migration!
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Congratulations to you and the rest of your team Steve!
Happy to share a pre-print of some recent work. This is our first time using bioRxiv. I suspect we will be using it more moving forward.
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Thank you to all the collaborators, co-authors, and mentors for all the help throughout this project! @JYang22638056, Dr. Josephine Mitchell, @englishlauren2, Dr. Sihui Yang, Tanner Tenpas, Professor Erik Dent, Professor Jill Wildonger, and my advisor @erwright73!
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We tried so many different things to try to optimize this platform, and learned a lot of things along the way in regards to the difficulties in sample preparation for cellular cryo-ET (with special attention to neurons).
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But we encountered a LOT of difficulties along the way, with the biggest problem being that even though the neurons looked fine when grown on grids before freezing, when we put them in the EM they look like anything but.
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This project started as an attempt to establish an alternative primary neuronal cell culture model for cryo-ET, using Drosophila due to the versatility in genetic manipulation, low cost of maintenance, and fast reproduction rate.
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I would like to announce that my work on cryo-ET on Drosophila neurons is now published on @MAMtheJournal! #Tomography #TeamTomo
https://t.co/6dopsqjCbt
academic.oup.com
Abstract. Cellular neurobiology has benefited from recent advances in the field of cryo-electron tomography (cryo-ET). Numerous structural and ultrastructu
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While I wasn’t able to attend the last day cause I got ill, I just wanted to say it was a blast attending my first ever SfN conference here at DC! It was great seeing lectures where cryo-EM and cryo-ET got a shoutout, and meeting so many new people and old friends too! #SfN2023
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I may have developed a strong liking for french toast this summer.
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Great work, Qixiang!
Happy to share our review of the human telomere C-strand fill-in mechanism. Appreciate the opportunity to be involved in the process and learn a lot from @cijilim and the revision.
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Transferrin Receptor Targeting Chimeras (TransTACs) 🧬 The field of targeted protein degradation is going through a renaissance. The core idea: use a bifunctional molecule to bring a protein in proximity to a degrader. The first wave (PROTACs) primarily used E3 ligases as the
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Why are alcohol and opioids addictive, but not psilocybin or menthol? Why do fungi and plants make such chemicals in the first place? Why are many plants toxic to cats? Why did a Mexican yam lead to "The Pill"? Why did the Spice Trade hasten the Opioid Crisis? For answers...👇
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Nature research paper: Rewiring cancer drivers to activate apoptosis
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Now this is cool. CryoEM visualizes of an adverse drug interaction of an antiviral together with an anti arrhythmic in Cav1.1 and 1.3! Somehow I doubt this could’ve been predicted like this.
cell.com
Co-administration of sofosbuvir and amiodarone can lead to fatal heartbeat slowing, yet the underlying mechanism has not been clear. Analyzing six cryo-EM structures of Cav1.1 and Cav1.3 in the...
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Hi! It me! Uh I think I do biological research though…
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This was one Godzilla of a project. So glad that I can share this with the cryo-EM/cryo-ET community!
🎊Check out @UWMadisonChem & @UWBiochem student @jykim35 outstanding work developing #Drosophila #neurons as a model #cryoET system! A true tour de force with cell culture, grid prep, micropatterning, etc! 🥳 https://t.co/Jy6lgGWDRN
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Juan - The superstar Caulobacter flagella man!
🎉 Super excited to see @UWBiochem & @UWMadBiophysics student @_Juan_C_Sanchez🥇 first-author paper! An amazing #cryoEM structural journey with 3! Caulobacter #flagella! Providing insight into flagellin packing & multi-flagellin filaments. More to come!🎉
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Molecular basis of polyspecific drug and xenobiotic recognition by OCT1 and OCT2. The final version is out today on @NatureSMB
https://t.co/Usviz5FFWH
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I ran my first half marathon! Very happy I got to do this, thanks to everyone who came out to support me!
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