NatureStructMolBiol
@NatureSMB
Followers
10,259
Following
370
Media
567
Statuses
7,698
Nature Structural & Molecular Biology publishes top-quality research providing insight into how molecular components work together in biological processes.
Joined October 2015
Don't wanna be here?
Send us removal request.
Explore trending content on Musk Viewer
#RafahOnFıre
• 1471961 Tweets
Armageddon
• 645965 Tweets
#THEDANCEDAY
• 134853 Tweets
giselle
• 103416 Tweets
İsrail
• 91447 Tweets
#CDTVライブライブ
• 90564 Tweets
SAKAMOTO DAYS
• 63174 Tweets
Fermín
• 53580 Tweets
Pablo Iglesias
• 51490 Tweets
아마겟돈
• 50428 Tweets
#MemorialDay
• 44905 Tweets
Irene Montero
• 42664 Tweets
D.U.N.K
• 40142 Tweets
الجيش المصري
• 38125 Tweets
Maresca
• 34033 Tweets
世界さん
• 30723 Tweets
Leicester
• 19813 Tweets
ケイくん
• 19043 Tweets
蘭ちゃん
• 16905 Tweets
Jarabe
• 16299 Tweets
ハーフタイムショー
• 15808 Tweets
ダンスデイ
• 15005 Tweets
サカモトデイズアニメ化
• 14922 Tweets
Kabir is God
• 13958 Tweets
#مرزوقه_والمشاهير_بدر_المطيري
• 13298 Tweets
音楽の日
• 11070 Tweets
Ferran
• 10936 Tweets
Eurocopa
• 10425 Tweets
Pinned Tweet
2024 marks 30 years since we published the first volume of NSMB!
to celebrate, throughout 2024, we will be reflecting on the road covered & looking towards the future -
check out many of the wonderful pieces from our readers, plus some of our thoughts:
6
6
11
Anthony Roberts & co report a
#cryoEM
structure of dynein-2 complex, suggesting mechanisms behind autoinhibition and transport on anterograde IFT trains
With News & Views by Susan Dutcher
3
115
346
Can AlphaFold2 predict the impact of missense mutations on structure? Correspondence by
@kyliejwalters
& Gwen Buel:
4
79
271
AlphaFold2 and the future of structural biology: Patrick Cramer
@CramerLab
@mpi_bpc
discusses the recently launched
#AlphaFold2
by
@DeepMind
and how it will impact structural biology.
0
87
237
#cryoEM
structures of SARS-CoV-2 ORF3a, an ion channel conserved in coronaviruses and a potential new drug target for
#COVID19
. Work by
@SteveBrohawn
@Bautista_DianaM
@AbhayKot
@kerndm
@chrismhoel
et al.
@berkeleyMCB
4
60
143
Crowdsourcing success
@UWproteindesign
! Foldit players design f 56 new stable proteins from scratch, representing 20 different folds, including one not observed in natural proteins. Four newly determined structures validate the designs.
0
37
125
A
#cryoEM
structure of the active human melatonin receptor
#GPCR
in complex with Gi reveals conformational changes upon activation and basis for G-protein selectivity. Work from the Nureki, Inoue (
@TohokuSeika
) and Nishizawa labs led by
@hhokamoto_en
1
26
103
#cryoEM
structure of human mitochondrial RNase P bound to precursor tRNA reveals the molecular basis for first step of RNA processing in human mitochondria. Work by
@hauke_hillen
@BhattaArjun2p
@c_dienemann
@CramerLab
@mpi_bpc
1
31
103
🐱🧬AUGUST ISSUE IS OUT! 🧬🐱
X-chromosome inactivation - insights from the active and inactive X chromosome
We featured 3 papers on the cover, which investigate the molecular mechanisms of dosage compensation in mammals.
issue:
2
27
95
Biochemical and cell-based assays reveal that PARP inhibitors impede maturation of nascent DNA strands during replication and implicate unligated Okazaki fragments and other nascent strand discontinuities in the cytotoxicity of these anti-cancer compounds.
2
20
94
How poly(A) stops ribosomes in their tracks
0
39
92
#NMR
& direct visualization of phase-separated FUS and Pol II domains in models of transcription condensates show a wider array of residue types & interactions that stabilize phases. Work by
@Nicolas_Fawzi
@BrownUResearch
@CarneyInstitute
@TAMUengineering
0
21
90
Biochemical and cell-based assays reveal that PARP inhibitors impede maturation of nascent DNA strands during replication and implicate unligated Okazaki fragments and other nascent strand discontinuities in the cytotoxicity of these anti-cancer compounds.
1
19
85
NSMB Research Highlights this month shine the spotlight on mitochondria.
#1
"PINK spots: diseased mitochondria prepare for mitophagy" discusses 2 papers in
@Nature
&
@MolecularCell
by
@komander_lab
@gl_alisa
&al and
@jftrempe
&al on PINK1 activation mech
1
18
81
Nanodiscs rule! Lipid environment promotes GPCR–G protein extensive interactions, as shown for neurotensin receptor by the Wagner, Nasr &
@alanbrownhms
labs
@harvardmed
.
1
26
84
📸another gentle reminder for the ongoing cover competition! 📸
Want to be featured on NSMB cover? submit a spectacular photo, a beautiful piece of data, an insightful drawing to our 30th anniversary cover competition.
Deadline - end of November
6
36
79
#ICYMI
: David Baker & colleagues
@UWproteindesign
describe two de novo designed protein classes that link phosphorylation by tyrosine and serine kinases to protein-protein association and provide potential new avenues to regulate cell function.
0
23
83
NSMB assemble !
team meeting in Berlin - so great hang out in person
2
2
79
Check out our Special Issue on Ubiquitin Biology !
We feature some really exciting science on the mechanisms underlying writing and reading ubiquitylation + some very thoughtful comments and perspectives on the field
1
17
76
Work by
@helin_kristian
& colleagues shows that PRMT5 methylates the splice regulator SRSF1, causing changes in alternative splicing of multiple essential genes required for leukemia cell survival.
3
26
79
It’s not just a phase: in their review, Hannah Wiedner & Jimena Giudice discuss recent insights into the influence of phase separation on RNA biology
0
27
72
Want to be featured on NSMB cover? Submit a spectacular photo, a beautiful piece of data, an insightful drawing to our 30th anniversary cover competition.
Details:
0
22
70
.
@FandWLab
@HarvardCellBio
@HHMINEWS
report a structure of yeast seipin, a lipid droplet formation protein & suggest it forms a flexible cage in the ER
#membrane
, enabling triacylglycerol phase separation,
#lipiddroplet
growth & budding toward cytoplasm.
1
14
73
Zhou & colleagues
@NIEHS
show that DNA polymerase δ extrinsically proofreads errors made by both Pol ε and itself, and that the symmetry of
#replication
fidelity is achieved by cooperative efforts of intrinsic & extrinsic proofreading and
#DNA
MMR
0
26
72
Why is gene transcription associated with chromatin acetylation? Check out this study by Daniel Panne and colleagues just out in
@nature
0
39
68
@zhang_lab
@qinhuirao
@Yale
report impressive
#cryoEM
structures of the HUGE outer arm
#dynein
(OAD) assembly on the axoneme that drives rhythmic ciliary beating.
6
13
70
Piwi-directed heterochromatin: On the cover of our February issue, we highlight work by
@changwei_yu
@juliusbrennecke
&al
@IMBA_Vienna
on Panoramix SUMOylation on chromatin, connecting the piRNA pathway to heterochromatin formation. Full issue:
0
14
67
HAPPY 30th BIRTHDAY TO US🎂
First issue of NSMB came out in January 1994. Come celebrate with us 30 years of changes and progress in structural and molecular biology
We are continuing celebrations throughout the year with more content, so stay tuned!
0
18
65
Structure maps of RNAs localized to chromatin, nucleoplasm and cytoplasm reveal an intricate interplay of
#RNA
structure, RNA-protein interactions and RNA modifications
0
34
64
Crystal structure of a complete bacterial microcompartment shell, 6.5MDa!
1
48
62
NOMPC (TRPN) cryoEM structure, 29 ankyrin repeats! Insights into tether model for channel gating & mechanosensing
0
45
62
#ICYMI
:
#cryoEM
structure of the human SAGA complex reveals high-res details of core and TRRAP modules. Work by
@Nogaleslab
@berkeleyMCB
@UCB_Biophysics
@LBNLBioSci
@TjianDarzacq
0
10
62
Cryo-ET work from
@GaiaPigino
& co
@mpicbg
@humantechnopole
reveals the architecture of mammalian primary cilia.
3
16
62
.
@lapassmore
& colleagues show that Pan2 and the Ccr4–Not nuclease Caf1 recognize the intrinsic stacked, helical conformation of poly(A) RNA.
0
25
61
#cryoEM
structure of the human SAGA complex reveals high-res details of core and TRRAP modules. Work by
@Nogaleslab
@berkeleyMCB
@UCB_Biophysics
@LBNLBioSci
@TjianDarzacq
1
11
58
Chaperones too can aggregate! Work from
@buchnerlab_tum
on eye lenses from mouse strains with mutations in α-, β-, or γ-crystallins challenge the prevalent model for cataract formation.
#proteostasis
#chaperones
0
26
59
#CryoEM
work by Greiwe, Costa, Diffley &al
@TheCrick
shows how DDK selectively targets DNA-loaded MCM helicase to trigger
#replication
origin activation. Checkpoint kinase Rad53 blocks origin firing by impairing double hexamer engagement by DDK
#openaccess
1
18
58
TET2-mediated hm5C modification of tRNA affects the levels of tRNA fragments (tRFs), which have previously been linked to the regulation transposable elements, translation and epigenetic inheritance.
0
16
58
The case for post-predictional modifications in the AlphaFold Protein Structure Database: In this correspondence
@GABRAHREX
@2016Carl
@ElisaTelisa
@glycojones
present an approach to include post-translational modifications in
#AlphaFold
predictions.
0
10
55
Nanobodies for a wide range of research applications, ready in a matter of weeks through an improved yeast surface display platform
0
26
54
Deubiquitinating FANCI-FANCD2 - Structures of USP1-UAF complexes reveal molecular details of USP1-UAF1 regulation and substrate recognition
0
13
54
Tn7 transposition depends on its AAA+ ATPase adaptor TnsC. A new
#cryoEM
structure from
@alba_guarne
&
@shen_yao10
@mcgillu
shows how TnsC targets DNA sequences between target & insertion sites to regulate sequence-specific transposon insertion.
0
12
51
#CryoEM
structure of the 80S ribosome–Xrn1 nuclease complex reveals how the conserved core of Xrn1 allows binding at the mRNA exit of the ribosome, ensuring efficient mRNA degradation after the last round of translation.
0
25
50
DOPEGAL, a metabolite of noradrenaline in the locus coeruleus, covalently modifies Tau and accelerates its aggregation and propagation, leading to cognitive deficits in Alzheimer’s disease.
0
14
48
A study by Liz Carpenter and colleagues
@UniofOxford
@thesgconline
reports the first crystal structure of a human ELOVL revealing an unexpected reaction mechanism & suggesting potential approaches for inhibition to treat disease
1
19
47
The expressome, a supercomplex with actively transcribing RNA polymerase and translating ribosome, is seen in Mycoplasma pneumoniae cells, using integrative approach combining cryo-ET and crosslinking/MS analyses. From Mahamid lab
@embl
&
@RappsilberLab
0
15
50
Ingolia lab carried out a systematic survey of the budding yeast proteome and identified hundreds of post-transcriptional regulators. Regulatory activity often lies within intrinsically disordered regions
@nickingolia
@KendraReynaud
0
15
47
.
@juliusbrennecke
& colleagues identify a novel player in piRNA mediated transposon silencing - new tricks for an RNA export factor.
0
24
47
Paired-Seq - a new ultra high-throughput method for the parallel analysis of open chromatin and transcriptome in millions of single cells from Bing Ren & colleagues
0
27
47
On the cover of our August 2021 issue, we highlight recent work by Brandão, Ren,
@xindanw
@leonidmirny
and colleagues reporting beautiful Hi-C plots resembling historic tiles. Check out the full issue here:
2
7
47
Check out this collection of articles we helped put together for
@NaturePortfolio
in honor of
@DJuliusSF
and
@ardemp
, recipients of the the Nobel Prize in Physiology or Medicine 2021. Includes some amazing structural biology!
1
24
45
A fresh start: Meissner & colleagues
@MPI_MolGen
provide further evidence that Dnmt1 displays global de novo methylation activity, especially at retrotransposons
0
20
45
Target practice: first Piwi structure with RNA
In this short piece, we highlight recent work by MacRae lab
@scrippsresearch
published in
@Nature
working out how Piwi selectively finds its transposable element RNA targets with the help of piRNA guides.
0
11
46
❄️our January issue is live❄️
on the cover we feature work from
@davidwtaylorjr
&
@ArlenJohnson8
labs on how a single 2′-O-methylation of rRNA gates assembly of a functional ribosome
3
7
45
#CryoEM
reveals structural basis for coupling specificity of one
#GPCR
, the β1-adrenergic receptor, to two different families of G-proteins. Work by Xin-Yung Huang
@WeillCornell
@HiteLabMSKCC
and colleagues.
0
10
45
#cryoEM
structures of human ribosome nascent chain complexes stalled by the drug-like molecule PF846 reveal its mechanism of action and follow up screens show its context dependent function.
1
20
45
Combination of a bicistronic biosensor w/ single-molecule tracking & computational modelling reveals kinetics of cap- vs IRES-mediated translation & shows that IRES bursts are shorter & rarer than bursts of cap translation, which reverses upon stress
1
16
44
The life and scientific contributions from Aaron Klug, commemorated by Daniela Rhodes:
0
27
43
Here,
@JoshCofsky
,
@doudna_lab
, and colleagues, use
#CryoEM
and biochemical analysis to investigate how the
#CRISPR
associated protein
#Cas9
interrogates DNA to locate its RNA-matching target sequence
@berkeleyMCB
@igisci
0
13
43
🌊SEPTEMBER ISSUE IS ONLINE 🌊
We featured work from
@WilsonLab2
on the cover, where authors present structures of 17 antibiotics bound to E.coli ribosomes.
the full issue:
1
11
41
Our April issue cover features Strychnos toxifera, a plant from which the arrow poison curare can be extracted. Cryo-EM structures of muscle nicotinic receptors by
@RyanHibbs10
@MahfuzRahmanPhD
&al
@UTSWBrain
reveal its mechanism of action. Full issue:
2
7
41
#ICYMI
: Ribosome cryoEM structures by
@FujimoriLabUCSF
@fraser_lab
@shuramankin
&al w/ the antibiotics linezolid & radezolid bound to the peptidyl transferase center & adjacent growing nascent peptide chain provide explanation for context-specific action.
0
15
40
New work from David Ron's lab
@Cambridge_Uni
@TheCIMR
: Structures of the dephosphorylation complex of eIF2α-P reveal how contacts with the regulatory PPP1R15A subunit mediate substrate selectivity.
0
8
40
Live cell imaging of tagged genomic loci reveals clusters of distal enhancers in close proximity to target genes, creating a nano-environment wherein site-specifically recruited enhancer-associated regulatory factors activate transcription.
0
19
39
LiteMol suite: interactive web-based visualization of large-scale macromolecular structure data
0
29
39
Cryo-EM structures tell a tale of two telomerases: A News & Views by Michael D. Stone and colleagues
@ucsc
discusses two recent papers in
@nature
reporting
#telomerase
#cryoEM
structures by
@KellyTHD_Nguyen
and
@FeigonLab
0
12
38
Authors reveal architecture of the TRAP complex bound to the translating ribosome and Sec61 translocon, explaining its function in insertion and secretion of proteins
@matjaskol
@The_Ban_Lab
@DeuerlingLab
@jomaa_lab
@ETH_DBIOL
@UniKonstanz
@MedicineUVA
0
7
38
David Baker and collegues design transmembrane proteins, from monomers to tetramers, with up to 8 transmembrane segments in the oligomer.
1
24
37
Stretching the resolution limit of atomic force microscopy:
News and Views by
@hoogenboom_lab
highlighting and explaining an improved AFM technique called 'Localization AFM' developed by
@George_R_Heath
@ScheuringLab
recently published in
@Nature
0
9
37
Using
#cryoEM
, Christoph W. Müller & colleagues
@EMBL
@EMBLHeidelberg
structurally investigate elongating RNA polymerase I at 2.7 Å as well as an RNA polymerase I open complex at 3.3 Å and bound to initiation factor RRN3 at 3.2 Å.
0
6
36
Two papers on Chlamydomonas cilia, from Vale lab
@HHMIJanelia
and from
@alanbrownhms
@harvardmed
& Zhang
@wustl
, provide unprecedented detail into the radial spoke architecture and new insights into mechanoregulation.
1
10
36
High-resolution cryo-EM structures of human ATM bound to ATPγS and two distinct ATM inhibitors provide insights into the mechanism of inhibitor selectivity and offer a framework for structure-based drug design.
0
6
35
Here, the authors use cryo-EM to solve the Arabidopsis thaliana Argonaute10 (AtAgo10)-guide RNA complex with and without a target RNA representing a slicing substrate, to provide insights into the slicing mechanism of a eukaryotic AGO
0
8
35
Out now
@NatureSMB
:
#cryoEM
structures by Darst & colleagues
@RockefellerUniv
&
#MDsims
@DEShawResearch
show how the SARS-CoV-2 nsp13 helicase and RNA polymerase function together within the viral replication-transcription complex.
1
6
35
Kern et al. show how LRRC8A:C volume regulated channels assemble into heteromers and use pore-bound lipids to block conduction in a closed state.
@kerndm
,
@SteveBrohawn
#ionchannels
#cryoem
1
7
34
#cryoEM
structures of human IMPDH1, a metabolic enzyme important in retinal function and disease, reveal polymorphic filaments. Blindness-associated IMPDH1 mutations are characterized & half disrupt feedback inhibition. Work by
@kollmanlab
@aniburr
&al
@UW
0
8
35
Beautiful 'butterfly' structure of RNase PNK reveals two molecular switches that coordinate nuclease and kinase function of this multi-enzyme pre-rRNA processing complex.
0
5
35
The structure of the SALL4-Pomalidomide-Cereblon-DDB1 complex provides insights for rational design of cereblon binding drugs with reduced teratogenic risk.
0
18
34
Dynamic changes in 2’-O-methylation of
#rRNA
in human cells lead to
#ribosome
heterogeneity and result in altered translation of select mRNAs, correlating with changes in cellular phenotypes. Work by
@therealLundLab
@UCPH_BRIC
1
7
34
Acetylation takes aim at mRNA: Choi & Meyer highlight a study by Oberdoerffer, Coller & co published in
@CellCellPress
, which identifies acetylated cytidine as a widespread mark in mRNAs that influences RNA stability and translation
1
23
34
#ICYMI
:
#cryoEM
structures of the metazoan GET complex which targets nascent tail-anchored membrane proteins to the endoplasmic reticulum reveal interactions that coordinate client transfer between two protein chaperones. Work from
@sshaolab
@harvardmed
0
4
34
#ICYMI
: PTEN is a key cell signaling lipid phosphatase that is regulated by C-terminal phosphorylation. Philip A. Cole and colleagues
@harvardmed
@BrighamWomens
@BWHGenetics
now used biophysical methods...(1/2)
1
6
34
Here,
@JoshCofsky
,
@doudna_lab
, and colleagues, use
#CryoEM
and biochemical analysis to investigate how the
#CRISPR
associated protein
#Cas9
interrogates DNA to locate its RNA-matching target sequence
@berkeleyMCB
@igisci
0
7
32
Cryo-EM and X-ray crystal structures reveal the architecture of the human Xkr8-Basigin
#scramblase
complex, providing insights into the molecular mechanism of phospholipid scrambling. Work by Shigekazu Nagata and colleagues
@osaka_univ
@osaka_univ_e
0
10
34
From the Urban lab
@JohnsHopkins
@sciencemagazine
: intramembrane rhomboid proteases distort surrounding lipids, allowing much faster diffusion than other integral membrane proteins.
Perspective by Wolfe
0
10
32
#ICYMI
: HDX-MS reveals that
#SARSCoV2
spike ectodomain reversibly samples an open-trimer conformation, revealing epitopes for a pan-coronavirus antibody. Work by
@ShawnMCostello
@MarquseeLab
&al
@UCBerkeley
@UCB_Biophysics
0
9
33
our current cover highlights work from
@GaiaPigino
lab on the molecular structure of IFT-A and IFT-B trains & anterograde transport of cargo into the cilia tip 🚂🚂
@samlacey745
@_Helen_Foster_
@humantechnopole
1
8
33
The winterly cover of our December issue features work by
@Dr_Ellisdon
@Dr_MLHalls
@MonashBDI
@LuptonCJ
@bj_charles
@sci_laura
et al. reporting the structure of the autoinhibited neurofibromin dimer, a large loop-shaped scaffold. Check the full issue here:
1
11
33
#cryoEM
structures of E. coli MutS at sequential stages of the ATP hydrolysis cycle reveal how ATP binding and hydrolysis and its modulation by DNA enable MutS to adopt different conformations during mismatch repair. Work by
@ABorsellini
&al
@LUMC_Leiden
1
9
32
Zagotta & colleagues at
@UWBiochemistry
use tmFRET and Rosetta tools to develop a model for the HCN channel VSD under conditions of membrane hyperpolarization.
0
9
33
.
@RyanHibbs10
@MahfuzR1985
&al
@UTSWBrain
reveal how the electric organ & skeletal muscle nicotinic acetylcholine receptor desensitizes in response to agonist & how the arrow poison curare antagonizes the channel by stabilizing a desensitized state.
0
6
30
We pay tribute to Michael Rossmann, an extraordinary structural biologist who was also an early advocate for data sharing.
0
14
32
2 papers in NSMB report
#CryoEM
structures of retinal rod and cone CNG channels, revealing 3:1 stoichiometry and a putative intracellular Arg gate.
Jian Yang &al
@Columbia
@jacopomarino10
@psich_en
@GSchertler
@caesarbonn
&al
0
4
31
News & Views by Dylan Taatjes
@CUBoulder
highlighting and comparing recent
#CryoEM
structures of the human transcription preinitiation complex (PIC) from the
@YuanHe_NWU
@CramerLab
and Yanhui Xu labs.
0
11
31
LOL is for "localization of lipoprotein": Haohao Dong
@SiChuanUniv
and colleagues reveal the structural basis for how the LolCDE system mediates transport of bacterial lipoproteins to the outer membrane
#cryoEM
0
11
31