Very happy that our work on multiplexing smFISH is out! With @SimoneCodeluppi & @slinnarsson. In the below gif you can see how the signal of 33 genes builds up over 12 cycles of osmFISH, every dot is one detected molecule of the corresponding gene. https://t.co/9HIkvvQzee

Historical chart from a review: #spatial #transcriptomics #Visium #HDST #GeoMx #APEXSeq #SlideSeq #seqFISH+ #osmFISH #STARmap #BaristaSeq #ProximID

Novel methods poster session including human #stemcell forebrain spheroids & osmFISH of >30 genes in mCx @LarsBorm #FENS2018

When your first publication is on osmFISH...

The high res osmFISH figures by @LarsBorm and @SimoneCodeluppi of @slinnarsson lab are poster worthy 😍 https://t.co/RXqHgMFQAC https://t.co/Iqb2HK1VoM

Shout out to @ProtocolsIO for building a great way to share protocols and increase reproducibility! Our osmFISH method is now available as a protocol:

Great discussions today about osmFISH -- a protocol used to measure transcript abundance in tissue sections. Link here: https://t.co/Gxm4F7D3aE via @LarsBorm

Spatial organization of the somatosensory cortex revealed by osmFISH https://t.co/E61G0iIvBf protocol: https://t.co/qgZKfENojt

Regev #AGBT19 To learn intrinsic, extrinsic features: insi2vec method: define a cell by both features. Cell image, define latent space. osmFISH data '18 Nature Methods

Awesome osmFISH is out! Congrats to @LarsBorm @SimoneCodeluppi et al.! ”Spatial organization of the somatosensory cortex revealed by osmFISH | Nature Methods”

Spatial #transcriptomics methods are developing very rapidly. No clear dominant method yet, like @10xgenomics . In 2019, which would you try to bring into your lab and why? MERFISH, seqFISH, osmFISH, PLISH, SABER, FISSEQ, STARmap? #neuroscience #tx #genomics #singlecell

@AdamHantman Cool. There a number of emerging methods (STARmap, MERFISH, seqFISH, SABER, osmFISH). It's at the point where single cell was in like 2013-2014. It's tough to know which will be the widely adopted one and which to invest in. Like single cell, spatial methods will change rapidly.

Awesome osmFISH!

Wrapping up a fantastic week working with the Allen Institute and the SpaceTx consortium! - getting image-based transcriptomics data from a variety of assays (smFISH, RNAscope, BaristaSeq, ISS, osmFISH, SeqFISH, DartFISH, MExFISH) processed through common analysis pipelines

SL: OsmFISH technique looks beautiful in multiplex. Would love to know more about negative controls and RNA structure effects. More reading #HGM2018

Very interesting method using osmFISH to study single cell transcriptomics from cells within the cortex, while keeping the tissue intact and therefore gaining spatial information as well! Recommended! Poster H009 from Lars Borm today at #FENS2018 @FENSorg

@Dr_RajatGupta @cziscience @JCoolScience @DBBurkhardt @BrianBlackUCSF @anshulkundaje @Prof_Lundberg osmFISH from @LarsBorm @SimoneCodeluppi @slinnarsson

After a lot of fun work with @LarsBorm in @slinnarsson lab the osmFISH paper is out: https://t.co/ef0ciI1Hza Dig around https://t.co/CgPRooBJqs for high res img and code... proud of it!

SL: towards cellular resolution of maps of cell identity - OsmFISH https://t.co/MRVxeFto7p #HGM2018

Finding publicly available spatial transcriptomics data that is complete with the requisite raw data and metadata is extraordinary difficult. Sharing is possible, e.g. https://t.co/aTVPLxbi5t from the @slinnarsson lab is great, but sadly it is the exception rather than the rule.