@MariuszCzC @11_Shuv_52 I wish to thank all the coauthors of this study and our colleagues from Nowotny lab. We are also grateful to @IIMCB_Poland, @NCN_PL and @SOLARIS_science for making this project possible!.

@MariuszCzC @11_Shuv_52 Finally, we deliver the first complete cryo-EM structure of a two-component recombinase composed of ICP8 annealase and UL12 alkaline nuclease. The complex was reconstituted using a model DNA with a dsDNA and 3′ ssDNA overhang.

@MariuszCzC @11_Shuv_52 We are also presenting a cryo-EM reconstruction of a double filament of ICP8 at 3.2 Å resolution. We visualize the interactions between protein subunits within and between the antiparallel strands. DNA used to produce the filaments could not be traced.

@MariuszCzC @11_Shuv_52 A very similar DNA conformation is observed in the crystal structures of ICP8 with 14-mer ssDNA. ICP8 subunits form dimers with a two-fold symmetry, with their DNA-binding sites facing each other and the outward-rotated bases of bound DNAs form pairs in a flexible manner.

@MariuszCzC @11_Shuv_52 We determined a cryo-EM structure of BALF2 bound to ssDNA at 3.2 Å resolution. The visible fragment of DNA is accommodated in a positively charged cleft. In the first four nucleotides, the base edges face the protein, but the next three bases are rotated away from the protein.

I am very excited to share our recent preprint in which we are structurally investigating macromolecular complexes that contain DNA-binding proteins ICP8 of Herpes Simplex Virus 1 and BALF2 of the Epstein-Barr Virus.

We are very happy to share the preprint of our latest study on the DNA-binding proteins from Herpes Simplex Virus-1 and Epstein-Barr Virus! @IIMCB_Poland.

Following up on our paper about AbiK and Abi-P2, we now present an in-depth characterization of the third Abi polymerase, AbiA, performed in collaboration with @hauryliuk lab and @DziembowskiLab.

Great job, congratulations to Shivlee and Mariusz!.

RT @IIMCB_Poland: ➡We are starting recruitment for #Warsaw4PhD within @IIMCB_Poland .➡We offer the opportunity to join 2️⃣ unique projects….

So happy that I was granted funding for this awesome project!.

RT @MariuszCzC: Ever heard of trimeric reverse transcriptases? Check out our new paper from @MarcinNowotny lab with cryo-EM and MX structur….