RT @Immunobiology22: 🚨 Abstract submission deadline: July 15th 2025🚨.Do not miss the opportunity to present your research: 10 selected shor….

Congratulations to Doctor Alexandra Boegli who very successfully defended her PhD on Listeria activation of NLRP6 through induction of lysosomal damage 🥳!! Very well deserved (and a very well enjoyed celebration) 🥂

What do you want to know?.

RT @RebsamenLab: Join us in Lausanne October 1-2 for the 2nd edition of the Symposium on the Immunobiology of Pattern Recognition Receptors….

RT @FankhauserCh: Great job opportunity at The Center for Integrative Genomics, Lausanne University, Switzerland Fo….

RT @JWVeening: New tenure track assistant professor position in molecular microbiology in our department @DMF_UNIL @unil! We are casting a….

RT @Immunobiology22: We are excited to announce the 2nd edition of the Symposium on the Immunobiology of Pattern Recognition Receptors, Oct….

Registration opens soon for the second Pattern Recognition receptor symposium in Lausanne Switzerland in October 2025. Check out the link for the great list of speakers.

Congrats to @EBernard1993 for winning the poster prize at the Swiss LS2 conference for his work on the activation mechanism of Ninj1 during cell death!! 🥳 @DIB_UNIL @LS2Switzerland

Together, these data demonstrate that NLRP6 initiates a canonical inflammasome and induces pyroptosis, establishing NLRP6 as a sensor for damage to the endolysosomal system.

Finally, sterile endolysosomal damage alone, using the lysosome rupturing compound LLOME, is sufficient to activate NLRP6.

On the bacterial side, bacterial PAMPs aren’t sufficient to activate NLRP6. However, cell to cell spread is important for activation as a mutant deficient for making actin tails is a worse activator of NLRP, as is the plcB mutant deficient in escape from a secondary vacuole.

By comparing NLRP6 and 3, they map the upstream region of the NACHT domain - the FISNA and its conserved poly basic motifs- as being important for receptor activation, but despite sequence similarities they show that each receptor is activated by distinct ligands.

Then they show that listeria based activation of NLRP6 in HIEC intestinal epithelial cells has all the hallmarks of canonical inflammasome activation including GSDMD cleavage.

First they show that WT listeria - but not the dhly mutant which doesn’t express the pore forming toxin Listeriolysin O that allows the bacteria to escape from endolysosomes - are sensed in a HEK reconstitution assay.

Check out our latest pre print! @AlexandraBoegli and colleagues show that the enigmatic inflammasome receptor, NLRP6 is activated by sterile and pathogen induced endolysosomal damage. Hot off the press!.

RT @CellCellPress: Now online! How NINJ1 mediates plasma membrane rupture and why NINJ2 cannot

RT @Lo_Zanzi: #NoTimeToDie! @inflammasomelab, Holley, Monteleone &co show @NatImmunol that cells that die by #pyroptosis, or #necroptosis,….

RT @IshanDeshpande: An excellent summary by @EBernard1993 and @broz_lab , contextualizing our recent work.

RT @IshanDeshpande: Thrilled to share our findings on NINJ1 autoinhibition, now online in @Nature. Big thanks to the amazing team @dixitvis….

Did you know that NINJ1 exists as an autoinhibited dimer in the PM of cells? In a @NatureNV @EBernard1993 and Petr describe new results from @dixitvishva and @IshanDeshpande, including a resting structure of NINJ1 with 3 helices! Check it out here: