I’m excited to share an R package that we’ve created for mapping scRNA-seq data across normal and malignant hematopoiesis! . We've used this to identify hematopoietic stem cells from healthy tissues and map leukemia cells from hundreds of patients. 1/6.

RT @UHN_Research: A study published today in @NatureCancer from @PMResearch_UHN and @StJudeResearch has mapped how human blood #StemCells c….

B-ALL is a disease of B-cell development, but where exactly does this process get derailed?. For some patients, it’s earlier than you might expect and involves cellular states capable of both lymphoid and myeloid differentiation capacity!. See @IlariaIacobucc1’s tweetorial here:.

RT @PMResearch_UHN: Understanding leukemia cell development in AML🩸.Our recent study revealed 12 cell states as AML cancer grow and develop….

RT @AACR: Researchers have developed a map of normal hematopoietic cell differentiation and used it to examine how aberrant differentiation….

Excited to see BoneMarrowMap out in press! . Please check out the R package (now capable of classifying 100,000 hematopoietic cells in 10 minutes) and consider giving the paper a read for some insights into how differentiation goes wrong in AML!.

RT @UHN_Research: 🚨 Breakthrough in #leukemia research!. Dr. John Dick & team @UHN have mapped how leukemia cells grow & change, uncovering….

RT @BCD_AACR: Read the commentary: Putting AML Differentiation States on the BoneMarrowMap, by Jonas Berger and Livius Penter, online now c….

RT @BCD_AACR: Online now concurrent with #AACR25 #hematooncology Minisymposium talk by @andygxzeng and Dr. John Dick team @PMResearch_UHN:….

RT @BoWang87: DeepSeek-R1 is great, but it still does not handle genomics yet :) . 🚀 We’re thrilled to announce that IntegrAO (pronounced "….

RT @tjflemin: Stem cell-like states confer poor outcomes in blood cancer—but what mechanisms drive this and how can they be therapeutically….

Our tutorials teach you to map scRNA-seq data from normal and malignant blood samples within minutes. Alternatives: .I don't like Azimuth due to low HSPC resolution, but here are some other great atlases: .

We've also developed a focused map of human B-cell development spanning fetal and post-natal tissues, used to map 89 B-ALL patient samples. While most cells were Pro-B, there was involvement of multipotent cell states in a subset of B-ALL patients. 5/6

This map can be used to visualize how hematopoiesis becomes distorted in leukemia. We mapped scRNA-seq data from hundreds of AML patients and identified recurrent patterns of AML differentiation. These were linked to underlying genetic mutations. 4/6

So, we decided to create a scRNA-seq reference atlas with even proportions of primitive stem and progenitor cells and differentiated immune cells. Importantly, we annotated the cells to be concordant with FACS-purified HSPC fractions, as well as highly purified stem cells. 3/6

If you’ve performed scRNA-seq on leukemia samples, you’ll be know that analysis can be a chaotic process. Inter-patient heterogeneity dominates clustering even after batch correction, and cell type annotation of individual leukemia cells is also really difficult. 2/6

RT @BridgeKatherine: Delighted to share the first study from my group: ."HIF-1 activated by PIM1 assembles a pathological transcription com….

RT @marcobuttigieg_: On the very 1st day of my PhD, I’m so excited to share my work exploring #CH #CHIP in the tumour microenvironment, out….

RT @asger_jakobsen: Mutant HSCs in human clonal haematopoiesis have an attenuated response to inflammation and ageing, which may give them….

RT @PMResearch_UHN: Stem cell competition in human bone marrow. New study in @CellStemCell has found that resistance to inflammation gives….