Tanner Fadero
@TanFad
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(he/him) Light Microscopist. Tweets about microscopy 🔬, cell biology, and optics. No new posts here, but find me on bluesky: @tanner-fadero.bsky.social
Redwood City, CA
Joined August 2017
We are excited to announce the release of @manager_micro v2.0.2_20230815, which includes a new feature in the “On-The-Fly Processor Pipeline” menu: Deskew! Broadly, this is useful for automatically deskewing, rotating, and projecting light sheet images in Micro-Manager. (1/7)
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I'm not on Twitter anymore, but if you are, you should follow this account. Or come over to bsky and follow us there!
First post. NIH and medical research are under attack by the Trump admin. Cancer cures are being stopped. Please follow us for more info.
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Alright, I'm calling it. I'm over to the other place (rhymes with clue guy). Find me at my new profile: tanner dash fadero at bee ess kay why dot social dot com.
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We present long-term stabilization of an oblique plane microscope (OPM). By stabilizing the remote focus unit of an OPM, we can enable long-term acquisition without light-sheet drift. The feedback signal comes from a laser, no fluorescence is expended. https://t.co/bu503wSmDO
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Correction from @TanFad! It's actually the The Center for Advanced Light Microscopy (CALM) @UCSF that is building the #Snouty microscope! They have a great team there with @nico_stuurman, @KariHerrington and @micaelalasser (Instagram). A great location!😀
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PSA: Heat-set inserts make stronger threads in a 3D print, and look quite tidy.
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And thanks to @amsikking for running a thermal calibration on this exact King Snout lens!
Here I measure the axial shift of the focal plane of an AMS-AGY v2 objective (a.k.a KingSnout) at ~2.0um/K, with a CLTE of ~19e-6/K (similar to brass): https://t.co/1ELPBNAmqg
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Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all: https://t.co/OAImWfbEHi Feedback always welcome. https://t.co/dYkihjPuL8
We (@AndrewGYork, @RunningPhoton, @microRussell and I) are still working on the manuscript describing our automatic parameter-free deconvolution approach, but we've made a Google Colab notebook where you can try it out without needing to install anything: https://t.co/7NpsTQrwBO
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Made a prototype light trap out of aluminum shim stock and Musou black paint. Watching paint dry has never been so interesting. The matte finish is fantastic!
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Did you know that nuclear pore complexes can turn up outside the nuclear envelope? Studying these, @JennySachweh, Bernhard Hampoelz and others uncover that the surrounding cellular milieu determines NPC composition via the nuclear transport system itself. #teamtomo #NPC A 🧵
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Where is the optical axis? Finding it can be critical to building microscopes. Here's the back reflections at ~15cm from a collimated ~1mm⌀ 532nm laser aligned into the back of Nikon 40x0.95 air objective. Radially symmetric/concentric interference rings indicate good alignment.
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Here are the specs for anyone interested: Mounting Threads: M48 x 0.75 Effective Focal Length: 28 mm Working distance: 29.3 mm
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Gentle Rhodamines for Live-Cell Fluorescence Microscopy | ACS Central Science
pubs.acs.org
Rhodamines have been continuously optimized in brightness, biocompatibility, and color to fulfill the demands of modern bioimaging. However, the problem of phototoxicity caused by the excited...
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Woah cool! I'm going to try this O2/O3 mount for my snoutscope build at the @UCSF CALM
This corresponds closely with the O2-O3 'cross brace' solution from @JLazzariDean. Based on the above data, reversing one of the POLARIS-K2T mounts will neutralize their thermal effect, leaving just the aluminum cross brace and an ~exact cancelation: https://t.co/yULf8o71Pk
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Does anyone know what the effective focal length (EFL) and/or the mounting threads of @NikonInst's standard LWD 0.52 NA condenser lens is? Pic for context:
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@RetoPaul @bingy_C But I'm glad that it seems possible to build a motorized slit for automatic light sheet NA control out of stock parts. Tagging relevant builders: @amsikking @kD3AN @MinesBioImaging @astormic @JLazzariDean
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