
Tanner Fadero
@TanFad
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(he/him) Light Microscopist. Tweets about microscopy 🔬, cell biology, and optics. No new posts here, but find me on bluesky: @tanner-fadero.bsky.social
Redwood City, CA
Joined August 2017
We are excited to announce the release of @manager_micro v2.0.2_20230815, which includes a new feature in the “On-The-Fly Processor Pipeline” menu: Deskew! Broadly, this is useful for automatically deskewing, rotating, and projecting light sheet images in Micro-Manager. (1/7)
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RT @amsikking: Correction from @TanFad! It's actually the The Center for Advanced Light Microscopy (CALM) @UCSF that is building the #Snout….
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And thanks to @amsikking for running a thermal calibration on this exact King Snout lens!.
Here I measure the axial shift of the focal plane of an AMS-AGY v2 objective (a.k.a KingSnout) at ~2.0um/K, with a CLTE of ~19e-6/K (similar to brass):.
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RT @JamesDManton: Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter….
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RT @Beck_Laboratory: Did you know that nuclear pore complexes can turn up outside the nuclear envelope? Studying these, @JennySachweh, Bern….
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RT @amsikking: Where is the optical axis? Finding it can be critical to building microscopes. Here's the back reflections at ~15cm from a c….
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Woah cool! I'm going to try this O2/O3 mount for my snoutscope build at the @UCSF CALM.
This corresponds closely with the O2-O3 'cross brace' solution from @JLazzariDean. Based on the above data, reversing one of the POLARIS-K2T mounts will neutralize their thermal effect, leaving just the aluminum cross brace and an ~exact cancelation:.
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Does anyone know what the effective focal length (EFL) and/or the mounting threads of @NikonInst's standard LWD 0.52 NA condenser lens is? Pic for context:
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@RetoPaul @bingy_C But I'm glad that it seems possible to build a motorized slit for automatic light sheet NA control out of stock parts. Tagging relevant builders:. @amsikking @kD3AN @MinesBioImaging @astormic @JLazzariDean.
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