A major part of my PhD is out! 🎉 In this preprint, we introduce single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry. 1/n 👉 https://t.co/7wsWbOvozl

Felix Zhou's-Segment3D is out: https://t.co/ozZoqgDFiH It leverages 2D cell segmentations from orthoviews to generate a ‘consensus’ 3D segmentation. It does a great job on segmenting densely packed samples, and we have used the algorithm in many applications.

Fantastic PhD opportunity in beautiful Yorkshire! Amazing people and cool microscopes.

Always there for our group meetings!

Hey Mitochondria-tweeps, do you have any recommendations for Python-based mitochondria segmentation tools? Is there a Python version of the Mitometer? Any suggestions are welcome! https://t.co/Z8kEyn2bVz

New preprint from our lab! ER geometry sets the tempo of neuronal bursting via Ca²⁺ store-refill—revealing selective neuronal vulnerability to ER-structure problems. Thread to follow.🧠⚡️ #Neurodegeneration #NeuroCellBiology #Calcium #bioRxiv https://t.co/SKthEnJ8ue

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues. https://t.co/aoIjYvXEKx

Mary Brunkow, Fred Ramsdell and Shimon Sakaguchi have been awarded the 2025 Nobel Prize in Physiology or Medicine for their groundbreaking discoveries concerning peripheral immune tolerance that prevents the immune system from harming the body. The Nobel Prize laureates

Trying some new dyes! Was supposed to be ER specific. (Live COS7 cells labelled with two different ER probes).

First preprint as 1st author with @LabPhysiology out! The team and I are pleased to present: "A Vertically Integrated System for Tracking and Assessing cell-cycle aware phenotypes under confinement"

Interferometric Image Scanning Microscopy with adaptive pixel-reassignment for imaging of intracellular organelles in live cells with resolution fairly close to what SIM achieves. https://t.co/dR9SeNyxqV

Wishing a happy, healthy and sweet New Year to everyone celebrating Rosh Hashanah in the Cambridge community 🍯🍏

Just finished the chariots of fire race and ran twice for the team. Glad that I was a part of Mill Road running club team.

I am wholly in support of this proposal. BioRxiv and its cousins are all we need, and editor-dictated peer review has wreaked untold damage on the scientific enterprise since its widespread adoption in the 60s.

We really wanted to do cool science!

🚨 We are hiring a #postdoc in Quantitative Cell Biology/Biochemistry. Join us if you are interested in using super-resolution STED & cryoET to uncover self-organization dynamics of epithelial cell interfaces & glycobiology collaboration with WuLab https://t.co/NsZS1X7iPo

خسوف امشب عکس از کاوه سیداحمدیان

4D lattice light sheet movie on MOSAIC of dividing pig kidney cells, with an example of tripolar mitosis starting in the middle of the frame at 7 sec. A volume rendered snapshot of the three daughter groups in anaphase is shown at right.

Huge thanks to an incredible team who made this possible 🙌 especially @AleksPonjavic for his great supervision and support. 6/n

We applied smFC to triple-negative breast cancer (TNBC) cells: 📊 Found 30% of cells express low-abundance c-kit (1–200 molecules/cell). 🧬 This subpopulation was invisible to standard flow cytometry, but smFC revealed it. 5/n

With smFC we achieve: ✅ Digital precision down to ~2–3 molecules/cell ✅ 10–80× improved detection limits vs. conventional FC ✅ Quantification of membrane proteins in high throughput, single-cell detail 4/n