This semester I am teaching in a course called Mathematical Foundations of Quantitative Biology. It involves combinatorics, distributions, calculus (ODEs and PDEs), linear algebra and Fourier Series and Transforms. Taught on blackboard. Excited to do some #math .

Thrilled to share our Science cover! VIPS (volumetric imaging via photochemical sectioning) eliminates working-distance limits for whole-mount, nanoscale imaging. LLSM + photodegradable hydrogel + petabyte-scale compute → axons & myelin across mouse olfactory bulbs (WT vs NPC1).

"I Sure Am a Fan" is a high-octane, aggressive hard rock anthem that throws the listener directly into the boots of an American infantry soldier during an intense urban gunfight. The track's gritty, first-person lyrics describe the "sweat on my face" and chaotic action of

I knew they had a camera drone in the air, so I was hoping for some other angles of the final descent and flip maneuver. This is pretty amazing footage, wow. cc @Eric_Betzig

@amsikking 3D tracking of mitochondria DNA (green dots) in a HeLa cell.

@amsikking Volumetric imaging of 384 wells in a contact free format using an air lens. Importantly, the 3D imaging remains diffraction limited and distortion-free, despite the large refractive index mismatch from air to water.

Our "9/11/01 Miracle," my nephew, FF Michael Roberts found, during his funeral service

@amsikking Segmentation of nuclei and nucleoli in an expanded liver tissue:

@amsikking Movie time: microvili formation and dynamics in T-cells.

@amsikking Lastly, the zoom lens allows to adapt miOPM to samples with refractive indices (R.I.) different from water, such as cleared tissues. Below is a comparison of doing CLARITY cleared imaging, with and without adapting the system to the different R.I.

@amsikking We can even use an air objective and still perform distortion free and diffraction limited imaging in watery samples. This can be leveraged to perform contact-free multi-well imaging.

New driver in the house? Keep the lessons, skip the big bills.

@amsikking The same system equipped with a water immersion objective can image larger samples, like an expanded liver slice, organoids or neurons on an EM grid.

@amsikking You can use a high numerical oil immersion objective, and perform sensitive live-cell imaging, which we have leveraged to look at T-cell dynamics and track mitochondria DNA in 3D.

@amsikking came up with AIRR; it is an extension of remote focusing theory (Botcherby) in the presence of a refractive index mismatch. In practical terms, one can image distortion-free into a medium different from the immersion of the objective. This give miOPM its flexibility.

Unlike prior lightsheet systems, miOPM can use a variety of objectives, can be adapted to a wide range of refractive indices and is compatible with standard sample mounting. This comes from 2 innovations; Any Immersion Remote Refocusing (AIRR) and a motorized zoom lens.

Very excited to be at @WSUCougarFB for Game Day this week! @patcashmore @Coach_Pavlik @WazzuRogers @ChapfootballAZ @Trace_Carroll @CoachAbt77 @steverausch17 @Chris_Sailer @KohlsKicking @therealbeadle @JUSTCHILLY @MaxprepsAz @kt_recruiting @gridironarizona @azc_preps

Unlike prior light-sheet systems, miOPM can use a variety of objectives, can be adapted to a wide range of refractive indices and is compatible with standard sample mounting. This comes from two innovations; the any immersion remote refocusing concept, and telecentric zoom lens.

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues. https://t.co/aoIjYvXEKx

#HappyFluorescenceFriday! #microscopycommunity- don’t forget to submit your images to BINA’s Light Microscopy Image Contest- closes Monday 9/29! Win cool prizes - and bragging rights! More info: https://t.co/K7CemGbPGO

Belated Happy Zond5 day! 57 years ago, the first earthlings (two tortoises) circumnavigated the moon aboard the Zond 5 spacecraft: https://t.co/wCn5zvvIzd The re-entry was rather rough, so humans would not have survived it. The tortoises stayed alive.

We’re focusing on the “Cactus Creek Book Series” of novels by Janice Cole Hopkins next! Just go to our profile page and feel free to shop from these 5 titles!

Happy Triple Square day! 3^2, 4^2, 45^2

@SpaceX Starship landed!

@SpaceX A little toasty.