Many thanks to the @AACR #TMEWorkingGroup for highlighting our work on #glioblastoma #gbm!

Thanks @AACR for another incredible annual meeting! #AACR25 had it all: from the latest advances in cancer research & patient care to innovative technologies & oustanding representatives of the cancer community! Always grateful to @AACR @Novocure for supporting our #gbm research!

Thanks to @pathologynews & @ChristosEv88667 for highlighting our @CellReports paper on the SVZ microenvironment in #gbm #glioblastoma patients & the opportunity to discuss future research focused on in vivo targeting of IL-1β/IL-1RAcP & Wnt-5a/Frizzled-3!.

Thanks all! 🙏.

Finally published!! Thanks to all patients for the generous donation of tumor samples 🙏 and to the amazing members of my lab & collaborators who made this work possible @brainhekate @bryanwgranger @LeoGarMont @Varelasamantha1 @MichaelKogan20 @StefanoBerto83 @bowersbrainsurg.

Back to Albuquerque after a super inspiring and motivating #SNO2024! It was great to meet old and new friends and colleagues! So proud of @brainhekate and @LeoGarMont who presented our work on #GBM #glioblastoma covering genomics, bioinformatics and cancer stem-like cell biology!

It was an incredible meeting! Thanks to @TheMarkFdn @sontagfdn and the organizers for the invitation. Two days of outstanding scientific sessions, great panel discussions, ideas & reflections to advance #glioblastoma #gbm research and develop better treatments for patients!.

Super excited to visit @UofIllinois and @IGBIllinois and present our work on #gbm #glioblastoma intra-tumor heterogeneity! Thanks @PedronHaba for inviting me!

It was a real pleasure to contribute to the 5th Neuro-Oncology Symposium of @PASNO_Group. I enjoyed every interaction with faculty, staff, students and colleagues! Thanks to @AtherEnam, the meeting chairs, the organizers, the students, @PASNO_Group @official_pans @braintumourpk!

Excited and honored to speak at the 5th Annual Neuro-Oncology Symposium! Thanks to @PASNO_Group @AtherEnam @official_pans @braintumourpk for the invitation! Can’t wait to be in Karachi!.

RT @MichaelCovell_: 🚨#Neurosurgery Residents, Med Students, & Post-Doc Fellows🚨. What does #Mentorship mean to you? What goals & attributes….

RT @IvyBrainTumCtr: Tomorrow is National #WearGrayDay! Encourage your friends and family to go gray for the day and bring awareness to brai….

(12/12) In conclusion, we showed that in #glioblastoma #gbm the T_SVZ represents a distinct microenvironment characterized by a mesenchymal signature & tumor-supportive microglia. We identified 2 pathways, IL-1β/IL-1RAcP & Wnt-5a/Frizzled-3, as T_SVZ-specific therapeutic targets.

(11/12) Specifically, in vitro treatment of cancer stem-like cells with IL-1β and the anti-IL-1RAcP fully-humanized monoclonal antibody Nidanilimab significantly reduces cell proliferation. Moreover, treatment with Box5, a Wnt-5a antagonist, significantly decreases cell invasion

(10/12) Lastly, IL-1β/IL-1RAcP and Wnt-5a/Frizzled-3 are significantly upregulated in the T_SVZ microenvironment and represent T_SVZ-specific therapeutic targets

(9/12) Fourth, microglia establish cell-to-cell interactions with tumor cells in the T_SVZ microenvironment and are predicted to express IL-1β and Wnt-5a

(8/12) Third, we identified tumor-supportive microglia as the majority of tumor-associated macrophages in the T_SVZ microenvironment and showed that they spatially co-exist with tumor cells

(7/12) Second, we found that the T_SVZ microenvironment harbors tumor cell populations characterized by a ZEB1-centered mesenchymal signature & a distinct regulon profile of microglia

(6/12) First, we built a single-nucleus RNA-sequencing-based microenvironment landscape of the SVZ (T_SVZ) & tumor mass (T_Mass) of 15 #glioblastoma #gbm patients, and used 2 histologically normal SVZ (N_SVZ) samples as controls

(5/12) What are the key features of the SVZ as a tumor microenvironment? To answer this question, we used our previously published fluorescence-guided multiple sampling scheme