Rory Power
@Rory_M_Power
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Engineer @ EMBL Imaging Centre, EMBL Heidelberg @rory-m-power.bsky.social
Heidelberg
Joined June 2018
RT @adam_k_glaser: New TITANITE 5.0X / NA 0.27 microscopy lens now available from Schneider-Kreuznach!. Purpose-built ExA-SPIM lens with st….
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Anyone interested in developing light sheet technologies towards real service applications as part of ARISE2, please get in touch.
🌟 Exciting news! 🌟. The EMBL #ARISE2 Fellowship Programme, funded by @MSCActions, is now open for applications! This unique opportunity trains researchers to lead #ResearchInfrastructures & innovate in #LifeSciences. 🗓️ Join our webinar on 30 September:
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Well I guess I'm just going to need to get better at keeping all my python packages playing nicely together. no worries I'd gotten too lazy. @anacondainc terms and conditions aren't playing so nicely with academic insitutions it seems.
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Anyone been using the large (e.g. 20 mm) @Thorlabs galvos and can comment on flatness? . Any better options for a large aperture 1D scanner with high flatness? Can sacrifice some speed. no problem.
thorlabs.com
Thorlabs designs and manufactures components, instruments, and systems for the photonics industry. We provide a portfolio of over 22,000 stocked items, complimented by endless custom solutions...
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RT @NatureProtocols: #FeaturedProtocol this week is a comprehensive guide to building and operating a custom 3D #singlemoleculelocalization….
nature.com
Nature Protocols - The buildup and operation of a custom single-molecule localization microscope with state-of-the-art performance and advanced features bridges the gap between entry-level...
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Everything you need to build your own 3D multi-color, automatable single molecule localization microscope with full biological validation. Plus lots of rainbow CAD! @JonasRies @aline_tschanz @EMBLHeidelberg.
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Delighted to have this finally out with @nicola_gritti and @huiskenlab. IR light sheet provides better deep tissue contrast in fixed samples. Deep learning allows fixed (IR) data quality in live (GFP) samples.
nature.com
Nature Methods - InfraRed-mediated Image Restoration (IR2) uses deep learning to combine the benefits of deep-tissue imaging with NIR probes and the convenience of imaging with GFP for improved...
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RT @JonasRies: Heroic effort by @Rory_M_Power: he developed a detailed step-by-step protocol with all blue-prints for anyone to copy our au….
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Want to build switchable dual sided-light sheet illumination with pivot-based stripe suppression using only a single moving element? Improved illumination uniformity and imaging speed compared with previous mSPIM Check it!. @huiskenlab @Anjalie_Sch.
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Delighted to see this in pre-print form! A lot of blood and sweat went into this one; NIR light-sheet-images as ground truth to restore image contrast at depth in paired GFP-based live imaging data:.
biorxiv.org
Time-lapse fluorescence microscopy is key to unraveling the processes underpinning biological development and function. However, living systems, by their nature, permit only a limited toolbox for...
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Great work from Robert Prevedel's lab on mesoscopic OPM for imaging freely behaving animals.
We’ve joined the #OPM community! Our latest preprint describes an open-top, dual-objective OPM optimized for ‘mesoscopic’ FOVs >1.5mm that’s capable of imaging behaving Nematostella with cellular resolution. Great collaboration with @AissamIkmi @embl .
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Any OPM/optics folks have a view on this paper?. Seems to violate conditions for remote focusing for reduced O2-O3 tilt. Is strict adherence overkill at low NA?. @florianstroehl @RetoPaul @AndrewGYork @amsikking @JamesDManton @adam_k_glaser.
pubmed.ncbi.nlm.nih.gov
The proposed mesoscopic scanning OPM allows using low NA objectives such that centimeter-level FOV volumetric imaging can be achieved. With the extended FOV, simple sample mounting protocol, and the...
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RT @embl: Join us for 'Enabling imaging across scales' on 31 May 2022 at EMBL Heidelberg. Want to know more about the EMBL Imaging Centre….
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Any good Python tutorials suited to instrument (microscope) control out there? Slowly getting myself round to the idea. GUI development/concurrent tasks/device interfacing etc.
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No need for me to travel to for Seeing is Believing these days but its virtual anyway so no excuses to miss it.
Abstract deadline coming up! Share your insights on imaging and its applications in the life sciences by submitting your abstract by 3 August. #EESImaging.➡️
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Yes, I know the fibers are convenient, keeping the beams co-aligned, easy to remove laser from system without misaligning etc. The fiber engines are pretty spendy though and the variable fiber NA with wavelength causes some issues.
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Thinking through some laser options for fluorescence microscopes. I always gravitated towards laser engines and fiber launch but start to see some benefits of individual units and free-space. What's your preference?.
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