Zach Marin
@zacsimile
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hardware x software in microscopy | views are graphical projections | postdoc in Ries Lab @MaxPerutzLabs | @zacsimile.bsky.social | 🇺🇸 in 🇦🇹 | he/him
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Joined January 2017
These and many other simulations are covered in the paper. Example notebooks for each simulation are provided in the code base, and on Google Colab (see . We hope the community will find this useful and contribute their own examples using this package.
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For single molecule tracking, we optimized laser power, pattern size, photon limit and pattern dwell time to increase the measurable diffusion coefficient by 70% over that captured by the standard Abberior tracking sequence.
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We simulated DNA-PAINT imaging using an Abberior sequence file, and showed that quenched DNA-PAINT probes may yield better images than standard probes in MINFLUX.
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Fluorophore flickering can create up to a tenfold increase in localization precision. We showed this can be mitigated by repeated scanning of a fluorophore.
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Starting off simple: misalignment of the phase pattern that generates a donut degrades performance, while misalignment of the pinhole has little effect.
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MINFLUX works well in theory, but system and sample imperfections can lead to suboptimal resolution. To address this, @JonasRies and I developed SimuFLUX: a realistic simulator to optimize MINFLUX experiments before samples go on the microscope.
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Fluorophore flickering can create up to a tenfold increase in localization precision. We showed this can be mitigated by repeated scanning of a fluorophore.
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Starting off simple: misalignment of the phase pattern that generates a donut degrades performance, while misalignment of the pinhole has little effect.
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RT @RetoPaul: Our manuscript on accelerating OPM / SCAPE acquisition rate via aliasing is online now:. Our work ad….
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RT @bewersdorflab: 4Pi-SMS and pan-ExM reveal, in a close collaboration with the Rothman lab, a surprising tetraplex organization and self-….
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RT @ppa007hot: I am happy to share our 5-year's work of manual mapping of all the native proteins in the golgin family, providing the highe….
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RT @DrAbhishekSau1: I’m excited to share our latest research from @musser_lab , published today in @Nature. Using two-color 3D MINFLUX, we….
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RT @LukasScheiderer: 🎉Just out in @NaturePhotonics : Our Review describing the revolutionary principle behind #MINF….
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RT @RetoPaul: Our manuscript for remote focus stabilization for oblique plane microcopy is online:. .
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RT @YimingLi_SZ: Excited to share LUNAR (Localization Using Neural-physics Adaptive Reconstruction) —a self-supervised, blind localization….
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