Florian Ströhl
@florianstroehl
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*This Twitter account is not monitored any longer. Please join me on LinkedIn for continued research updates.* --- Florian, PI in Advanced Imaging
The Arctic University (Norway)
Joined October 2016
RT @JamesDManton: This is _clearly_ one of the best ideas in imaging of the decade. I strongly encourage people to play around with this i….
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RT @JamesDManton: We present:. 'Multispectral live-cell imaging with uncompromised spatiotemporal resolution'. 1/….
biorxiv.org
Multispectral imaging is an established method to extend the number of colours usable in fluorescence imaging beyond the typical limit of three or four, but standard approaches are poorly suited to...
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Recording many image planes simultaneously is a great time saver for 3D imaging. A wonderful tool for such multifocus microscopy is @FourierPower's Multifocus Grating: Here is a short video on how they work and how to make them (without a cleanroom).
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You can read the full paper (open access) at Finally, thanks a lot to Jon-Richard Sommernes and @amsikking - it was a pleasure working with you on this project! 4/n
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If you want to figure out the theoretical limits of your own LSM system, we also provide a software tool that can simulate any light-sheet microscope. The tool has a GUI and is straightforward to use. 3/n
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We have found that s-polarized light-sheets not only decrease the 3D PSF size by up to 30% but can also increase the light-efficiency of the whole system by up to 10% compared to p-polarized illumination light. 3/n
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To figure out how pronounced that effect is, we used vectorial diffraction simulations to evaluate the impact of a range of parameters, like light-sheet inclination, illumination polarization, fluorescent anisotropy, or the O3 immersion medium. 2/n
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Snouty-style OPMs offer volumetric imaging with high spatial- and temporal resolution, but the setups can suffer from polarization-dependent light reflections which reduce the effective aperture and transmission in the remote image space. 1/n
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RT @aandr314: How to draw optical layout / schematics?.First, start with vector graphics tool. Inkscape is free and good. Secondly, there a….
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Here is the original SOLIS paper that explains how the method can capture entire optically sectioned volumes at the camera framerate:.
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What a start to 2024. Nobel Prize winner @Eric_Betzig and his team not only built a SOLIS system. No, they built 4! .
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RT @haesleinhuepf: 🤫 #chatGPT integration will be coming to @FijiSc's Script Editor #SneakPreview 🥳.Thanks for the support @ctrueden ! 🙌.h….
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RT @JamesDManton: The slight disappointment with being scooped is more than compensated by the very nice results and demonstrations of redu….
nature.com
Nature Biotechnology - A dual illumination method reduces photobleaching for green and yellow fluorescent proteins.
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Here's how we did it: of course with complementary YouTube video:
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Fantastic work by Hari Shroff's group on 4-beam SIM.
An impressive experimental demonstration of 4-beam 3D-SIM for enhanced axial resolution, by Hari Shroff's lab:
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Remember Avatar, the first 3D movie? Yeah, that wasn't 3D. Actual 3D needs more than polarising glasses. Let's develop a real 3D imaging system together, shall we? Details in our newly published Optica paper 🥳:
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It's written in the stars: there's a new fluorescence microscopy paper coming from the Arctic University :)
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James nails it: the best reason to enjoy the good view from your lab is "alignment purposes".
I'm occasionally asked for tips on ensuring that a camera is properly axially located in the focal plane. People often regret asking that question when they see my quick-and-dirty (but accurate!) method that involves focussing on a distant object with the objective removed. [1/5]
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