I’m thrilled to share my postdoc research investigating the identity and control of regions of the mammalian small intestine. All FIVE of them! Introducing: small intestinal domains A – E. a 🧵:

RT @DrShrutiNaik: Misinformation is winning not because it's true, but because it’s loud, emotional, and everywhere. It’s time for science….

RT @BrisaPalikuqi: Tomorrow, together with @lydiagrmai, @thuycke and @RKZwick we will talk about Zoom Interviews! How to prepare and what t….

RT @BrisaPalikuqi: Join us today for session 3!! Together with @ScienceyRosalie, @ClarissaChemist and @RKZwick we will share tips about how….

RT @s_viragova: Very excited to share our review out today in @CellStemCell, which examines the reactivation of fetal-like programs during….

RT @BrisaPalikuqi: I am excited to announce that I have accepted a tenure track Assistant Professor position at the Yale Stem Cell Center a….

RT @NatureCellBio: 💫NEW: @OphirKlein, @RKZwick & co examine longitudinal transcriptional patterns across the whole mouse and human #SmallIn….

@NatureCellBio @s_lefkopoulos Thank you all for these incredibly nice responses! Your excitement is so appreciated.

Our work to define the regional organization of the small intestine is online @NatureCellBio today: ! Make way for domains A – E and a new approach to track the molecular patterns of nutrient metabolism. Many thanks to @s_lefkopoulos.& our reviewers!

RT @jmoorescience: So grateful for the support from @JCellBiol to attend ASCB! I’m excited to present some of my thesis work in a tandem ta….

RT @SItzkovitz: Some science during these terrible days: @KerenBaHa paper is online in @NatMetabolism: See also th….

It's wonderful to see the excitement about this work - thank you so much everyone for sharing and for your kind words!!.

P.S. 🔊Open call (/competition?) for more fun nomenclature than small intestinal domains A-E until time of publication!.

🍻to an incredible team in @OphirKlein lab esp postdoc community who moved mountains for some epic expts @WeixLaura @s_viragova @BrisaPalikuqi @cmcginnis92 P.Kasparek @karalmckinley + wonderful collaborators Dario Boffelli, @SItzkovitz, R.Locksley, @ZevGartner, @gardnerlabUCSF.

Domains are important! Different functions & propensity for disease. Hope this helps 1. lay groundwork to distinguish cellular 'neighborhoods' 2. precisely track regional adaptations in response to diet, disease+ & 3. one day,✨regenerate specialized function in damaged guts✨8/.

So, 53 feet of tissue & 315 scRNAseq samples later, the duodenum, jejunum, & ileum, once useful, are now a thing of the past. I’m excited to provide concrete molecular framework for 5 intestinal domains. Track ‘em in tissue or in silico w/our domain identity-trained classifier 7/.

How are domains controlled? We predicted the factors that pattern them and made CRISPR-modified regional organoids to modulate them as a start to answering that question. Also, we find the positions of 3 regional stem cell populations that likely seed them 6/.

So how many regions are there? 3? 30? After extensive analysis, we found 5 distinct domains. Domains = intestinal regions inhabited by enterocytes that co-express genes that encode distinct aspects of nutrient absorption, and have individual responses to 🥯🍗🍕🍤 5/.